Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,419,933 G→T A112D (GCC→GAC)  hisJ ← histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,419,9330GT98.5% 195.4 / NA 66A112D (GCC→GAC) hisJhistidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (35/30);  minor base A (1/0);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTA  >  CP009273/2419795‑2420076
                                                                                                                                          |                                                                                                                                                
tCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAg                                                                                                                                        <  2:245011/149‑1 (MQ=255)
tCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCCCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAg                                                                                                                                        <  2:808740/149‑1 (MQ=255)
  aaTGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGGCTGTCGGCTGAATGTCAGAATTTGTCGCCACCACCAAAGGAGAATCGTCAGCGTACAGtt                                                                                                                                      >  2:122767/1‑149 (MQ=255)
  aaTGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGtt                                                                                                                                      <  2:408417/149‑1 (MQ=255)
  aaTGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGtt                                                                                                                                      >  2:64194/1‑149 (MQ=255)
   atcccTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCACAATTTTTCGCCACCCCCAAACGAGAATCGTCAGCGTACAGttt                                                                                                                                     <  2:730371/146‑1 (MQ=255)
     gCCTTTTGCTGCCCAATGTCCATTACCTAACGTCGCCTGGCTCGCCCCCTGCAATACGCCTACCCGTGTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGt                                                                                                                                   <  2:67504/149‑1 (MQ=255)
      ccTTTTGGTGCCCAATGTTCATTACCGACCGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCCGCGTACAGTTTGTc                                                                                                                                  <  1:1059153/149‑1 (MQ=255)
       cTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCg                                                                                                                                 <  2:248913/149‑1 (MQ=255)
       cTTTTGGTGCCCAATGTTCATTACCGAACGTCTCATGGGTGGTGCCCTGCAATACGCCTACCCGATTGCCTTTCAGCGACTCGACTGGCGGCGGAATGTCAGAATTGTTCCCCACACCCAAAAGAGCATCGAGCGCGTACCGtgtgcgg                                                                                                                                 >  1:267279/1‑146 (MQ=255)
       cTTTTGGGGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCg                                                                                                                                 <  1:327505/149‑1 (MQ=255)
          ttGGGGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCATCAAACGAGACTCGTCAGCGTACAGTTTGTCGGTg                                                                                                                              <  2:580346/149‑1 (MQ=255)
                  cAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAAAGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTAt                                                                                                                     >  2:758717/1‑149 (MQ=255)
                        tCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACCGTTTGTCGGTGAAGGCTATTTCTTg                                                                                                               <  2:577232/149‑1 (MQ=255)
                         cATTCCCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGc                                                                                                              <  1:828055/149‑1 (MQ=255)
                         cATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTgg                                                                                                              >  1:1142679/1‑148 (MQ=255)
                         cATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGc                                                                                                              >  2:881377/1‑149 (MQ=255)
                            tACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGa                                                                                                           <  1:959629/149‑1 (MQ=255)
                             aCCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGAc                                                                                                          >  1:160740/1‑149 (MQ=255)
                             aCCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGAc                                                                                                          >  2:125008/1‑149 (MQ=255)
                                    gTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTc                                                                                                   <  1:470003/149‑1 (MQ=255)
                                      ctcCTGGGTGGTGCCCTGCAATACGCCTCCCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCg                                                                                                 <  2:196088/149‑1 (MQ=255)
                                       tcCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGCATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGCCGTTTTTCCGt                                                                                                >  2:1852/1‑149 (MQ=255)
                                           gggTGGTGCCCTGCCATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCAGTAATg                                                                                            >  1:732584/1‑149 (MQ=255)
                                               ggttccctgttgtACGTCTACTCGTTTGCCTTTCAGCCGTCCGATTGTCGGGGGAATGTCGGAATTTTTCGCCACAACCAAACGAGATTCCTCAGCGTACAGTTGGTCGGTGAAGGCTATTACTTTCTGACGTTTTTCCGTAATGGaaa                                                                                        <  1:449999/137‑1 (MQ=255)
                                                gtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAg                                                                                       >  1:1017888/1‑149 (MQ=255)
                                                 tgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGc                                                                                      >  1:282359/1‑149 (MQ=255)
                                                  gCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGaccgcg                                                                                     >  1:739169/1‑144 (MQ=255)
                                                    ccTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGAt                                                                                   <  2:891131/149‑1 (MQ=255)
                                                    ccTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTCCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGAt                                                                                   <  1:906608/149‑1 (MQ=255)
                                                     cTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATg                                                                                  >  1:1034545/1‑149 (MQ=255)
                                                     cTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATg                                                                                  >  2:506525/1‑149 (MQ=255)
                                                             cGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCCGAATTTTTCGCCACCACCAAACGCGAATCGTCAGCGTACAGTTTGTCGGTTAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACatgatg                                                                          <  1:65852/149‑1 (MQ=255)
                                                              gCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATgg                                                                         >  2:625380/1‑149 (MQ=255)
                                                                    ccGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCaa                                                                   <  2:710570/149‑1 (MQ=255)
                                                                         tGCCTTTCAGCGACTCGACTGTCGGCTGAAGGTCGGGATTCGTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGGCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAAtcttc                                                              <  2:501174/149‑1 (MQ=255)
                                                                             tttCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGCATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCg                                                          >  2:290108/1‑149 (MQ=255)
                                                                             tttCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTAAAGTTTGTCGGTGAAGGCTATTTCTTGCTGCCGTTTTTCCGTAATGGAAAGCGATGCCATGATGGCGTCACTCTTCTTCg                                                          >  1:332287/1‑149 (MQ=255)
                                                                             tttCACCGACTCCACTGTCGGCCGAATGTCATAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCg                                                          <  1:799536/149‑1 (MQ=255)
                                                                                  gCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATAGCATGATGGCGTCAATCTTCTTCGCtttt                                                     >  1:376796/1‑149 (MQ=255)
                                                                                    gactcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTaa                                                   <  2:406140/149‑1 (MQ=255)
                                                                                     actcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACATTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAg                                                  >  2:764797/1‑149 (MQ=255)
                                                                                       tcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGa                                                >  1:56842/1‑149 (MQ=255)
                                                                                         gactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACg                                              >  2:642498/1‑149 (MQ=255)
                                                                                         gactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACg                                              >  2:642618/1‑149 (MQ=255)
                                                                                          actGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACgg                                             <  1:625380/149‑1 (MQ=255)
                                                                                            tGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTCAGGCCGGGa                                           >  2:426964/1‑149 (MQ=255)
                                                                                            tGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGa                                           <  2:856142/149‑1 (MQ=255)
                                                                                              tCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGGAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCATTTAAGGACGGCAtt                                         >  1:51357/1‑149 (MQ=255)
                                                                                                 gCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAAc                                      <  2:56842/149‑1 (MQ=255)
                                                                                                     aaTGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCAt                                  >  2:714720/1‑149 (MQ=255)
                                                                                                       tGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATcc                                >  1:361099/1‑149 (MQ=255)
                                                                                                         tCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAg                              >  2:297330/1‑149 (MQ=255)
                                                                                                             aaTTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTAGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCCATTTTAAGGACGGGATTAAGGCATCCAGCggc                          >  2:768943/1‑148 (MQ=255)
                                                                                                                ttttCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGAttt                       >  1:420012/1‑149 (MQ=255)
                                                                                                                 tttCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGAtttt                      <  2:494086/149‑1 (MQ=255)
                                                                                                                    cGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCAGAGCAAGACTTGTTCGTCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa                   <  2:32883/149‑1 (MQ=255)
                                                                                                                     gCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCAGACGATTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTGCTTCGCTTTTAAGGACGGGATTAACGCAGCCAGCGGATTTTCGAc                  >  2:100224/1‑149 (MQ=255)
                                                                                                                       caccaccaAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACaa                >  2:647732/1‑149 (MQ=255)
                                                                                                                           accaAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAACCGt            >  2:418947/1‑149 (MQ=255)
                                                                                                                           accaAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGt            <  1:506525/149‑1 (MQ=255)
                                                                                                                            ccaAACTATAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTa           <  1:370829/149‑1 (MQ=255)
                                                                                                                            ccaAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTa           >  2:741303/1‑149 (MQ=255)
                                                                                                                              aaaCGAGAATCGTCAGCGTACAGTTTGTAGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAACGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACa         >  2:564067/1‑149 (MQ=255)
                                                                                                                                  gagaATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGc     >  2:822035/1‑149 (MQ=255)
                                                                                                                                     aaTCGTCAGCGGACAGTTTGTCGGGGAAGGCTATTTCTTGCTGACGTGTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTa  <  1:331492/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                
TCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTA  >  CP009273/2419795‑2420076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: