Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,048,586 A→T V18E (GTG→GAG)  glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,048,5860AT96.1% 126.8 / NA 51V18E (GTG→GAG) glnGfused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (29/20);  minor base C (1/1);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCA  >  CP009273/4048446‑4048731
                                                                                                                                            |                                                                                                                                                 
gcgcCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCATTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCAAGCGCACGTTCAAGCCCCCAACgg                                                                                                                                           >  1:1791/1‑149 (MQ=255)
 cgcCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGa                                                                                                                                          >  1:817388/1‑149 (MQ=255)
  gcCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGGTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCACACGTCGTCCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGAt                                                                                                                                         >  1:563672/1‑149 (MQ=255)
   ccAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATg                                                                                                                                        <  2:172466/149‑1 (MQ=255)
   ccAGCCCGTCCATTCCCGGCATACGAATATCTGAAAGCAGCACATCCGGCGTTTTTCTCGCCAGCGCCTCCCGCACTTCTGCGCCGATCTCAAACGTCGTACAGGTTAAACATGCCCCAGCGAGCCCACGTTCAAGCTCCCAACGGATg                                                                                                                                        >  1:12740/1‑149 (MQ=255)
          gTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGAACTTCTGCGCCGTTCTCAAACGTCGTACAGGTCAAACCTGCCCCAGCAATCGCACGTTCAATCTCCCAACgtctgacactat                                                                                                                                 >  1:712550/1‑138 (MQ=255)
                     gCATACGGATATCTGAAAGCAGCACATCCAGCGTTTTGCTCGCCAGCGCCTCCTGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCTAGCTCCCGACGGATGGAATTATCGTCATCGACt                                                                                                                      >  2:628650/1‑149 (MQ=255)
                         aCGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCCGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTCTCGTCATCGCCCAccc                                                                                                                  >  1:251908/1‑149 (MQ=255)
                          cGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCa                                                                                                                 >  1:592755/1‑149 (MQ=255)
                                  tGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTAt                                                                                                           <  2:540262/147‑1 (MQ=255)
                                  tGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTAt                                                                                                           >  1:540262/1‑147 (MQ=255)
                                  tGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATcc                                                                                                         >  2:807854/1‑149 (MQ=255)
                                   gAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATccc                                                                                                        >  2:210501/1‑149 (MQ=255)
                                                ccGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAAc                                                                                           <  2:731726/149‑1 (MQ=255)
                                                   gCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCATTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTc                                                                                        >  2:667969/1‑149 (MQ=255)
                                                       tttGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCt                                                                                    <  2:625853/149‑1 (MQ=255)
                                                        ttGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCtt                                                                                   >  2:861424/1‑149 (MQ=255)
                                                          gCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGGGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCAAGACTATCCCTCATTGAATAAACGTCACCTTTa                                                                                 >  2:42637/1‑149 (MQ=255)
                                                          gCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGGCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTa                                                                                 <  2:563672/149‑1 (MQ=255)
                                                            tCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATCAACGTCCCCTTTAtt                                                                               >  1:1014751/1‑149 (MQ=255)
                                                               ccAGCGCCTCCAGCACTTCTGCGCAGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTcc                                                                            >  2:768616/1‑149 (MQ=255)
                                                               ccAGCGCCTCCAGCACTTCTGCCCCGTTCTAAAACGTCGTACAGGTTAATCCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTcc                                                                            <  2:12740/149‑1 (MQ=255)
                                                                cAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                                           <  2:592755/149‑1 (MQ=255)
                                                                cAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                                           <  2:449189/149‑1 (MQ=255)
                                                                  gcgcCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGa                                                                         <  2:85569/149‑1 (MQ=255)
                                                                   cgcCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGAt                                                                        <  2:977644/149‑1 (MQ=255)
                                                                      cTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTCTCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTCTTACCTGATAgc                                                                     >  1:911802/1‑148 (MQ=255)
                                                                         ccgccagtcactgcCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTAACCTTTATTTCCTGATGGGCAg                                                                  >  1:269908/13‑149 (MQ=255)
                                                                                tgtGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAcc                                                           <  2:507817/147‑1 (MQ=255)
                                                                                  tGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCga                                                         <  2:1036377/149‑1 (MQ=255)
                                                                                     gcCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGaa                                                      >  2:129187/1‑149 (MQ=255)
                                                                                         ttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                                  >  1:786424/1‑149 (MQ=255)
                                                                                         ttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                                  >  1:766187/1‑149 (MQ=255)
                                                                                                      tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGCATGCCCCGGcc                                     >  1:697865/1‑149 (MQ=255)
                                                                                                      tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCAGCGAATACCCACACTATCACTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCCAGAACTCGCTATGACCTAGcc                                     >  1:439754/1‑149 (MQ=255)
                                                                                                       aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGc                                            >  2:584591/1‑141 (MQ=255)
                                                                                                       aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGc                                            <  1:584591/141‑1 (MQ=255)
                                                                                                           gTTAAACCTGCCCCAGCGAGCGCACGGTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGCCTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATATGCAGGTAACCCGCGAACTAGGTATGCCCTGGCCAACTg                                >  2:253682/1‑149 (MQ=255)
                                                                                                            ttAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCTTCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGGTAGGCTGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTgg                               >  2:729033/1‑149 (MQ=255)
                                                                                                                tccTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa                           <  2:376523/148‑1 (MQ=255)
                                                                                                                aCCTTCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa                           <  2:450122/149‑1 (MQ=255)
                                                                                                                aCCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa                           <  2:817388/149‑1 (MQ=255)
                                                                                                                aCCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa                           >  1:377001/1‑149 (MQ=255)
                                                                                                                   tGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTc                        <  2:49531/149‑1 (MQ=255)
                                                                                                                     ccccAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCaa                      <  1:667969/149‑1 (MQ=255)
                                                                                                                                gcACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGaa           >  2:750928/1‑149 (MQ=255)
                                                                                                                                gcACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGCAAACTCAATTTTGCCTGaa           >  1:119146/1‑149 (MQ=255)
                                                                                                                                 cactgtCAAGCCCCCAACGGATGGAACCATCGTCACCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAAt          <  1:476553/144‑1 (MQ=255)
                                                                                                                                    gTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCt       <  2:786424/149‑1 (MQ=255)
                                                                                                                                    gTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGCTAAATTCAATTTTGCCTGAATGCt       <  2:766187/149‑1 (MQ=255)
                                                                                                                                         aGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCTGTATGCCCTGGCCACCTGCGAAATTAACTTTTGCCTGAAGCCTGacca  >  1:348698/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
GCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCA  >  CP009273/4048446‑4048731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑