Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,546,084	G→T	H18Q (CAC→CAA)	yjiC ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,546,084	0	G	T	100.0%	168.6 / NA	57	H18Q (CAC→CAA)	yjiC	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/29);  total (28/29)

GTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGC  >  CP009273/4545939‑4546226                                                                                                                                                  |                                                                                                                                               gTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGa                                                                                                                                             <  2:449828/149‑1 (MQ=255)  ttttCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCAGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAg                                                                                                                                            <  2:716711/149‑1 (MQ=255)       taattttgagctcGCTTTTTCCTTCTTAATTATTGTTATTGCCACGAGTTAGTGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCACATTTTTATTTACGTGTAAACCCACTGTCGACTATTGGGTGATGACGGAATTGAGCATCt                                                                                                                                       <  2:299668/137‑1 (MQ=255)       tAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCt                                                                                                                                       >  2:821810/1‑149 (MQ=255)       tAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCt                                                                                                                                       >  2:575205/1‑149 (MQ=255)       tAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCt                                                                                                                                       >  2:575442/1‑149 (MQ=255)         aTTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCAGATTTTAATATACGTGTAAACTAACTGTCGACTATTGGGTGATGAAGGAATTGAGGATATGt                                                                                                                                     >  2:716182/1‑149 (MQ=255)          tttCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGta                                                                                                                                    >  1:1029725/1‑149 (MQ=255)              gACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGAAATTGAGCATCTGtaataa                                                                                                                                >  1:651010/1‑149 (MQ=255)                    gCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGAc                                                                                                                          >  2:940597/1‑149 (MQ=255)                           ccATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGagg                                                                                                                   >  2:83235/1‑147 (MQ=255)                                  aaTTATGGCTACTCCTATGAGTAAGAGGCGGAAGTTAGATCTTTTCCGCATCCATGCGTGCCTATTTTAATGTACGTGTATACTCACTGTTGACTATTGGGTTATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTg                                                                                                            <  1:160642/149‑1 (MQ=255)                                       tGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTaa                                                                                                       >  2:32686/1‑149 (MQ=255)                                         gtttgtGCCACGAGTAAGAGGCCGAAGGTAGATGTTGTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCTACTATTGGGTGATTAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATg                                                                                                     <  1:88217/144‑1 (MQ=255)                                                 cACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGTTTAATGGCATAGAc                                                                                             >  1:465514/1‑149 (MQ=255)                                                       tAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATTAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                       >  2:509144/1‑149 (MQ=255)                                                             gCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAGGGGCATTGTTAAATTGCATAGACattagacgtacc                                                                                 >  2:235069/1‑139 (MQ=255)                                                                gAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCttt                                                                              >  1:945636/1‑149 (MQ=255)                                                                   gatggatgGTTTCCGAATCCAGGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTAt                                                                           <  1:481454/149‑1 (MQ=255)                                                                   gatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTAt                                                                           <  2:544760/149‑1 (MQ=255)                                                                       gatGTTTTCCGAATCCATGCGTGCATATTTTAAATTACATTTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGt                                                                       >  1:477339/1‑149 (MQ=255)                                                                        atGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTa                                                                      >  2:60909/1‑149 (MQ=255)                                                                         tGTTTTCCGAATCCATGCTGTCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTAt                                                                     <  1:963271/149‑1 (MQ=255)                                                                          ggtttCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTAtt                                                                    <  1:508698/147‑1 (MQ=255)                                                                          gTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTAGTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTAtt                                                                    >  2:464341/1‑149 (MQ=255)                                                                            tttCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTTGGTGATAAAAGAATTAAGCATCTGTAATAATTTGTCTCTGAATGGCATTGCTTAATGGCGTATACCTAATAGTTAACTTTTATGAGTAtttt                                                                  >  2:346813/1‑148 (MQ=255)                                                                             ttCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCa                                                                 <  1:398660/149‑1 (MQ=255)                                                                             taccgatgccATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTTATGAAGGAATTGAGCATCTGTAATTATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCa                                                                 <  1:49219/141‑1 (MQ=255)                                                                              tCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATACGAAATAATTGGACTATCAAGGACATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTAAc                                                                >  1:112672/1‑149 (MQ=255)                                                                               ccGAATCCATGCGTGTATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGCAATAAGTTGACTCGGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACt                                                               <  2:89773/149‑1 (MQ=255)                                                                                 gtattcATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCAACTAATGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACACCGAAGAGAATCGCTTAATGGCATAGACATAATAGTTAAATTATAAGAGAATTTCACTGa                                                             <  1:83235/144‑1 (MQ=255)                                                                                  aaTCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGAAATTGAGCATCTATAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTATTATGAGTATTTCACTGAt                                                            >  1:490656/1‑149 (MQ=255)                                                                                     ccATGCTTGCATATTTTAATTTACTTTTAAACTCACTGTCGACTATTGGGTGATTAATGAATTGAGCATCTGTAATAATTTGACTTTGAAGGGCATTTCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGtt                                                         <  2:93424/149‑1 (MQ=255)                                                                                               atatTTGAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATa                                               <  1:916336/149‑1 (MQ=255)                                                                                                 atTTTAATTCACGTGCTAACTCTCTGTCGACTATAGGGTTATGAAGGAATTGAGTATCAGTACAATGTGGCCTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGa                                             <  2:241247/149‑1 (MQ=255)                                                                                                   tttAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATa                                           >  2:430259/1‑149 (MQ=255)                                                                                                        tttACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttt                                      <  1:940597/149‑1 (MQ=255)                                                                                                        tttACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttt                                      <  1:752768/149‑1 (MQ=255)                                                                                                          tACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttttt                                    <  1:821810/149‑1 (MQ=255)                                                                                                            cGTGTAAACTCACTTTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCt                                  <  1:351617/149‑1 (MQ=255)                                                                                                               gtAAACTCACTGTTCACTATTTGGTGATTAATGAATTTAGCATATGTAATACCTTGCCTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATAATTAGAAAAATAGATAAATTTTTCTgta                               <  2:251947/149‑1 (MQ=255)                                                                                                               gtAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTATgta                               >  2:1027078/1‑149 (MQ=255)                                                                                                                tAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATTAATTTTTCTgtag                              >  2:333799/1‑149 (MQ=255)                                                                                                                     tCACTGTCGACTATTGGGCGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCATTGATTTTGAGAAAAATAGATAAATTTTTCTGTAGTaaaa                         <  1:85836/149‑1 (MQ=255)                                                                                                                      cccTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTaaaaa                        <  1:96372/147‑1 (MQ=255)                                                                                                                       aCTGTCGACTCTTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAACAGATAAATTTTTCTGTAGTAAAAag                       >  2:152294/1‑149 (MQ=255)                                                                                                                        ttGTTGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAaga                      <  1:566419/148‑1 (MQ=255)                                                                                                                         tGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAagag                     >  2:887160/1‑149 (MQ=255)                                                                                                                          ttCGAATATTGGGTGATGAAGGAATTGAGCAACTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCGTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAgaga                    <  1:469630/148‑1 (MQ=255)                                                                                                                          gTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAgaga                    >  1:853979/1‑149 (MQ=255)                                                                                                                           tCGACTATTGTGTGATTAAGGAATTGAGCATCTGTAATAATTTGACTTTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGaa                   <  2:674285/149‑1 (MQ=255)                                                                                                                           tCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGaa                   <  1:737562/149‑1 (MQ=255)                                                                                                                           tCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACATTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGaa                   >  2:356676/1‑149 (MQ=255)                                                                                                                              aCTAATGGGTGCTGATGGCATTGAGCATCTGACATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGCCATAATCCCTACCCTATCTGAGTTTTTCACCGATGTTTCGAAAAATAGATAAATTTTTCTGTGGCAAAACGAAAAGTa                >  1:122088/1‑149 (MQ=255)                                                                                                                                 aTTTGGTGATGAATGAATTGAGCATCTGTAATAATTTCACTCTTAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATTAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAAc             <  1:716182/149‑1 (MQ=255)                                                                                                                                 aTTGGGTGAGGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTGACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAAc             <  2:440277/149‑1 (MQ=255)                                                                                                                                        gatgaAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAc      >  2:389956/1‑149 (MQ=255)                                                                                                                                           gaaggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAACAGAAGTAAACAAATGAcatg   >  1:186723/1‑149 (MQ=255)                                                                                                                                            aaggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgc  >  1:340581/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                               GTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGC  >  CP009273/4545939‑4546226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑