| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,804,319 | T→C | 12.1% | intergenic (+14/‑149) | yniC → / → ydjM | 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,804,319 | 0 | T | C | 12.1% | 75.1 / 3.2 | 33 | intergenic (+14/‑149) | yniC/ydjM | 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA |
| Reads supporting (aligned to +/- strand): ref base T (16/13); new base C (2/2); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01 | |||||||||||
GCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTAT > CP009273/1804186‑1804466 | gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAACATAAACCCCGTTGTGTATTAGCAGACGCCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCGCGGTCAGTGACCAGGGACAGTGCCCTCGCt > 2:300656/1‑143 (MQ=255)gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCt > 1:510735/1‑149 (MQ=255) ccGCATGCGTTCCATCGTCGTTCCTGCGCCACAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTgg < 1:434466/149‑1 (MQ=255) cGCATGCCTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCTGGt < 1:283752/149‑1 (MQ=255) aTCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCCGCCATCTCGCTGCCCCCGGTTCTTTa > 2:208116/1‑143 (MQ=255) ttCCTCCGCCAGAAGCGCAAAATGATCCACGTCCTGGATTAGCAGGCGTCAAACTTTCATCGCTTACAAAACTAACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCTGGtcattaaccgagaa < 2:306352/143‑14 (MQ=21) tGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGc > 2:477975/1‑143 (MQ=255) gcCAGAAGCGCAAAATGATCGACGTTTTGTATTAGCAGACGTCGAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGACATTGCAtt > 2:39714/1‑143 (MQ=255) cgcAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCCGGTTCTTTATCTGAAATTGCATTCAACTGat < 2:346904/143‑2 (MQ=255) aTCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATc > 2:180528/1‑143 (MQ=255) tCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTGATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTGAACTGACGGATTAATCg > 2:294502/1‑143 (MQ=255) tttGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAgaga > 1:352212/1‑149 (MQ=255) tttGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTAGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCTTCAATTTAAgaga > 1:352176/1‑149 (MQ=255) ttGGATTTGCAGACGTTAAACTTGCATGGCCGACAGAACTCCCCGCAAAAGACCTCCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTTTTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTa < 2:190359/143‑1 (MQ=255) tATTAGCAGACGTCAAACTTTCATCACTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAaga > 1:264822/1‑149 (MQ=255) tAGCAGTCGTCAAACTTTCCTCGCTGACAGAACTCACCGCAAATGACCTTCTCGGTTAATCACCAGGGGCAGTGACCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGtt < 1:178674/149‑1 (MQ=255) cgCAGACGTCAAACTTTCACCGCCGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGaaa < 2:384408/142‑1 (MQ=255) gcggACGGCAAACTTTCATCGGTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGGCCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCTATTTAAGAGAAag < 2:288053/140‑1 (MQ=255) gACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGCTTAATCGTCAATTTAAGAGAAAGAGTTACAcc > 1:436866/1‑149 (MQ=255) aaaCTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACAcc > 2:309962/1‑143 (MQ=255) aaaCTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcccc > 1:438594/1‑149 (MQ=255) aCTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGt < 2:459364/143‑1 (MQ=255) tCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACt > 2:269136/1‑143 (MQ=255) cTGACAGAACTCACCGCAAAAGACCTCCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCAc < 1:510616/149‑1 (MQ=255) gACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGt > 2:200984/1‑143 (MQ=255) gACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACAGGGGGCAGTGATATCTTGGCCCCTGGTTCCTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTg < 1:101149/149‑1 (MQ=255) cAGAACTCCCCGCAACAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCCATTCACAATAAAGAGTTACACCGTCACCACTTCCGTGCACTGta < 1:258398/149‑1 (MQ=255) aaaaGACCTTCCCGGCTATTTACCAGTGGCAGTGATCTCGCTGCCCCCGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCtata < 1:200405/149‑1 (MQ=255) aCCTTCGCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTa < 1:76416/149‑1 (MQ=255) agataaagaaCCAGGGACAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAGTTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTCTAAAAACCCTATACTGtct > 2:455909/10‑141 (MQ=37) gTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGAc > 1:414288/1‑149 (MQ=255) ggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTACCGACAGTTTAg > 2:162444/1‑143 (MQ=255) gTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTAt < 1:208143/149‑1 (MQ=255) tGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACGGTCCGTATCGACAGTTTAGTGAGt > 2:321126/1‑143 (MQ=255) | GCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTAT > CP009273/1804186‑1804466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |