| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,328 | G→A | 52.9% | intergenic (‑61/+160) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,328 | 0 | G | A | 52.9% | 3.8 / 40.2 | 36 | intergenic (‑61/+160) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (10/7); new base A (8/11); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01 | |||||||||||
TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACA > CP009273/2420188‑2420471 | ttttGCGGAAACACATCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGATCCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaa < 1:139308‑M1/149‑3 (MQ=255) cagcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGGTAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAGTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatc > 1:275081‑M1/1‑134 (MQ=255) gcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaa < 1:314357‑M1/149‑18 (MQ=255) aaCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCCCCAGTTGTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGcgaca > 1:79105/1‑145 (MQ=255) gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATCATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGCTTGCTACACTTATCGTAAAATAAaattaattgaccatcga > 2:449931‑M1/1‑126 (MQ=255) gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgagcatcaa > 2:124444‑M1/1‑126 (MQ=255) cgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACCCTTATCGTGCCATAAAATTGCCCATCAAGGCaca > 2:390491/1‑141 (MQ=255) cgcTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgcc > 1:305916‑M1/1‑116 (MQ=255) gcTGGGAAAGGTCAGCACCAGAGAGAGCTATACCACCAGTTTTTGCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattaaccatcaaggcaaaatacc < 2:411349‑M1/143‑29 (MQ=255) cTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGcc > 2:444633/1‑143 (MQ=255) tGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg > 1:406440/1‑149 (MQ=255) ggAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGa > 1:413540/1‑149 (MQ=255) ggAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgt > 1:525408‑M1/1‑112 (MQ=255) aGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCTACTTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtat < 1:30790/149‑1 (MQ=255) aCCAGAGAGAGCGATATCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatt < 1:78480‑M1/149‑51 (MQ=255) ccAGAGAGAGCGTTAGCACCAGTTTTTTCTTTATGTCCTCAACTGGCAGTCTTTTTATAACGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattattt < 1:259877‑M1/149‑52 (MQ=255) ccAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtattat > 2:86711/1‑143 (MQ=255) agagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa < 2:17882/143‑1 (MQ=255) agacCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTACCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa < 2:339781/143‑1 (MQ=255) aGCACCATTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTaa < 1:397120/149‑1 (MQ=255) cATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTCTGCATTTGAAt > 1:129427/1‑149 (MQ=255) cATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCACAAAATTGACCATCAATGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATACTTAAGTATGCATTTGAAt > 1:318997/1‑149 (MQ=255) tCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAACGCTTAAGTATGCATTTAAATg > 2:217060/1‑143 (MQ=255) gACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcgaaatatcccgccccgttagacgtattatttttcattaaacatgcttacgtatgaatttccatgcccaa > 2:129255‑M1/1‑54 (MQ=255) tataGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatcgtgcaa > 2:454441‑M1/1‑43 (MQ=255) ggAATATTTACAGGTTAGTTGCACTTATCGTAACATAAaattaattgaccatcaaggcaaagtatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaac > 2:333787‑M1/1‑38 (MQ=255) gAATATTTACAGGTTTGCTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGc < 2:294257/143‑1 (MQ=255) tttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa > 2:377532/1‑143 (MQ=255) cAGGTTTGTGACCCTTATCGTAACATAAaattaatagaccatcaaggcaaaatctcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatga < 1:397017‑M1/149‑122 (MQ=255) aGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttctttaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctgccgtgcaccatgat < 1:361877‑M1/149‑123 (MQ=255) gTTTCTCACACTTATCGTAACATAAaattaatggcccatcaaggcaaaatatcccgccccgttagacgtattatttttcattacaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcacca < 2:426756‑M1/143‑119 (MQ=255) tttGTTACACTTATCAGGCCATAAAAGTGACCATCAATGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCAtt < 1:302645/149‑1 (MQ=255) ttACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatg < 2:270891‑M1/143‑124 (MQ=255) tACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatga < 2:475695‑M1/143‑125 (MQ=255) ttATCGTAATATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcatt < 2:213782‑M1/143‑130 (MQ=255) ctCGTGCCATAAATATTACCATCAAGGCAAAATTTCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATCCATTTCAATGCACTACGATAGTGCAACAAACCTGGCGAGCACCATGATGATCATTCCATCAGGTACa < 1:322059/148‑1 (MQ=255) | TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACA > CP009273/2420188‑2420471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |