New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 3780234 =63 (1.050)4 (0.070) 4/284 8.6 5.7% coding (37/1260 nt) envC activator of AmiB,C murein hydrolases, septal ring factor
?CP009273 3780286 = 72 (1.240)coding (89/1260 nt) envC activator of AmiB,C murein hydrolases, septal ring factor
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGCGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTA‑‑GATGATGGGCCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/3780382‑3780234
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcctTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCA  >  CP009273/3780286‑3780432
                                                                                                                                                                                                                                                                                                          
GCGCGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTA‑‑GATGATGGGCCT                                                                                                                                                     <  2:900375/149‑1
  GCGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTA‑‑GATGATGGG                                                                                                                                                        >  1:308381/1‑144
        TTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTGGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGGA‑‑GATGATGGGgcggat                                                                                                                                                  >  1:392726/1‑138
                                                                                               AAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTA‑‑GATGATGGGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCCAATCTATTCAGGCTGACATCGCCGCACAAGAGCCCGCGGTACGCCCCAAGCGACccc                                                           >  1:4917/1‑141
                                                                                                  GGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTA‑‑GATGATGGGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAAC                                                        >  1:606761/1‑144
                                                                                                             ATACGCCAGCGCTAAGAACGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCG                                         >  2:773845/1‑149
                                                                                                              TACGCCAGCGCTAAGAACGCTGGCGTATTGTTGTGCGCCTTTTCCGCCAACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCCCGGAACGCCAAAAGCAACAACAACCCGCCAGCCTG                                             >  1:844454/1‑144
                                                                                                                                                     CTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCCACACCGCGCGGGGTGCGCCAAAAGCGACAACAACGCGCAAGCCGGATCGCGCAATTGAAAAAGCAGGAAGAAGCGGTCTCTGAAGGCA  >  2:245304/1‑149
                                                                                                                                                     CTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCA  >  2:901269/1‑149
                                                                                                                                                      TTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAA      >  1:196478/1‑144
                                                                                                                                                                                                                                                                                                          
GCGCGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTA‑‑GATGATGGGCCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/3780382‑3780234
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcctTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCA  >  CP009273/3780286‑3780432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.