| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 704,377 | C→A | N196K (AAC→AAA) | chiP → | chitoporin, uptake of chitosugars |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 704,377 | 0 | C | A | 98.2% | 175.3 / NA | 55 | N196K (AAC→AAA) | chiP | chitoporin, uptake of chitosugars |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (35/19); total (36/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAA > CP009273/704242‑704521 | cATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACaa < 1:93214/144‑1 (MQ=255) aTTCAGCCCACTCGCCAAAGGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTATTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACaaa > 1:343777/1‑144 (MQ=255) ccAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCg > 1:141209/1‑144 (MQ=255) tcaCTGGTCAAACGCTGTTAGCTCCGCACTGGAGCTGTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGt < 1:785044/142‑1 (MQ=255) cAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTg < 1:514946/144‑1 (MQ=255) aaCGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGa > 1:81172/1‑144 (MQ=255) aCGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGa > 2:401388/1‑149 (MQ=255) aCGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAACGCCGTGGCATCTGGAAATGGa > 2:490275/1‑149 (MQ=255) gCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGTCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGATGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAAt > 1:704712/1‑144 (MQ=255) cgccgcACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGt > 1:352168/1‑144 (MQ=255) aCTGGGGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGACTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATc < 1:25750/144‑1 (MQ=255) aGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAAc > 1:889759/1‑144 (MQ=255) gCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGAACAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATaaa < 2:15150/149‑1 (MQ=255) cTTTATGCCAGGTACTTATCAGGGCGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATCTGGACCAAAGAATACAAAGCGCCGTGGCCTCTGGCAATGGATGAGTTCTATCAGAACGa < 1:694261/144‑1 (MQ=255) cAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTGCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTGATCAGAACGATAAAacac > 1:269746/1‑142 (MQ=255) aGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCTAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAaccac > 1:453580/1‑144 (MQ=255) ggTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAaccacc < 1:945158/144‑1 (MQ=255) aTCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTAt > 2:979401/1‑149 (MQ=255) cAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCt < 2:55086/149‑1 (MQ=255) aGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCACAGAATACAAAGCGCCGTGGCATCGGGAAATGGATGAGTTTTATCAGAACGATAGAACCACCAAAGGTGAtg > 1:639929/1‑143 (MQ=255) ggggCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCa > 2:405019/1‑149 (MQ=255) ggggCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCa > 2:269440/1‑149 (MQ=255) ggcgCGGAAGCCGGGGCGAATGTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGCATACAAAGCGCCGTGGCATATGGAAATGGATGAGTTTTATCAGAACGATAAACACACCAAAGTGGATTac > 1:715796/4‑143 (MQ=255) tcGGAAGCCGGGGCGAATTTTGATTACGGCGATGCCTGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTg < 1:247563/143‑1 (MQ=255) gAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTATCCTGAATGTGGAGAAAAGAAGGCAAAGGGGCGCGGCTGGTACTCAACCACGAGTTGTAGCAGAACTATAAAACCTCCAAAGTTGGTTATCTGCAc > 1:909291/1‑144 (MQ=255) aGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTg < 2:154976/149‑1 (MQ=255) gCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCtttag > 2:272847/1‑147 (MQ=255) gggCGAATTTTGATGACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTg < 1:796860/144‑1 (MQ=255) gAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGaact > 2:36641/1‑147 (MQ=255) aaTTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTCGGGCg > 1:775347/1‑144 (MQ=255) ttttGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACg > 2:788450/1‑149 (MQ=255) aCGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCaaa > 2:350331/1‑149 (MQ=255) aCGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCaaa > 2:350360/1‑149 (MQ=255) tGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCaaaaa < 1:248011/144‑1 (MQ=255) gTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTa < 2:740172/149‑1 (MQ=255) gCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTc < 1:648623/144‑1 (MQ=255) gAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAg > 2:914906/1‑149 (MQ=255) aGTTTCTCCTACATGGGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAACCCCCCAAAGCTGCTTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTg < 1:4334/144‑1 (MQ=255) gTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTCATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCg < 2:687997/149‑1 (MQ=255) gTTTCCCCTACATTTGGACCAAAGAATTCAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCg < 2:2819/149‑1 (MQ=255) tttCTCCTACATGTGGACCAAAGGATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGATGATTATCTGCATTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGa > 1:643535/1‑144 (MQ=255) tctcCTACGTGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAACCCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAg < 1:156168/144‑1 (MQ=255) ctcCGAGATGTAGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATATGCAATACTTTGGGGCGTAATACGACTGAAAAAATAACTTCGTACTGGAAGc > 1:475671/1‑144 (MQ=255) cccTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCg > 1:587621/2‑144 (MQ=255) ccTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATGATCTGCACTCCTTTGGGGCGAAAAACGACTTCAAAAATAACTTCGTACTGGAAGCGGCAttt > 2:701668/1‑149 (MQ=255) cTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAAGGATAAAACCACTATTTGATATTATCTGCACTCCTTTGGGGCGAAATACGACTTCCAAAATAACTTCGTACTGGAAGCGGCATTTg > 2:1014859/1‑149 (MQ=255) cTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTg < 2:621087/149‑1 (MQ=255) aTGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCa > 2:828005/1‑149 (MQ=255) ggACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCg > 2:999265/1‑149 (MQ=255) gACCAAAGAATACAAAGCGCCGTGGCAACTGGAAATAGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCa > 1:409862/1‑144 (MQ=255) aCCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAg > 1:721220/1‑144 (MQ=255) ccAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTTGAAGCGGCATTTGGTCAgg > 1:666285/1‑144 (MQ=255) ccAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGaa > 2:678749/1‑149 (MQ=255) cAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGc > 1:949407/1‑144 (MQ=255) cAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATAAAACCACCACAGTGGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAGCTTCGTACTGGAAGCGGCATTGGGGCAGGc > 1:875104/1‑144 (MQ=255) | CATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAA > CP009273/704242‑704521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |