Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 741,050 C→A L144M (CTG→ATG)  ybgL → UPF0271 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273741,0500CA98.3% 191.6 / NA 59L144M (CTG→ATG) ybgLUPF0271 family protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (27/31);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCA  >  CP009273/740910‑741189
                                                                                                                                            |                                                                                                                                            
gcggcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGa                                                                                                                                           >  1:73032/1‑144 (MQ=255)
gcggcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGt                                                                                                                                      <  2:783497/149‑1 (MQ=255)
gcggcgTAAGGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGa                                                                                                                                           <  1:61106/144‑1 (MQ=255)
   gcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCCTCGCCAGAGCGGTAGACGCGTGCGATCCAGCCTTGATTCTCGTCGGGCTGGCGGGGCGCGAGCTGGTTc                                                                                                                                        >  1:807638/1‑144 (MQ=255)
      tAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTg                                                                                                                                     <  1:182464/144‑1 (MQ=255)
      tAATGCGGCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTg                                                                                                                                     <  1:418035/144‑1 (MQ=255)
          gCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAgg                                                                                                                                 >  1:634508/1‑144 (MQ=255)
          gCGTCATGACAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGgcaagc                                                                                                                            <  2:829948/149‑1 (MQ=255)
               aTGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGgcaagc                                                                                                                            >  1:641194/1‑144 (MQ=255)
                 gTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATgg                                                                                                                     >  2:551553/1‑149 (MQ=255)
                  tCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGGCGGGCTGCCGGGAGAAGAGATGATTCGTGCAGGCAAGCAATATGGt                                                                                                                    >  2:138985/1‑149 (MQ=255)
                    caaCCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCt                                                                                                                  <  2:305875/148‑1 (MQ=255)
                       ccGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGt                                                                                                                    >  1:13327/1‑144 (MQ=255)
                           aCGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGa                                                                                                                <  1:835135/144‑1 (MQ=255)
                            cGGCATGTTGTACAACCAGGCGGCGAAAGACGCCCAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAAcgc                                                                                                         <  2:448786/149‑1 (MQ=255)
                              gCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATAGGGTCTGACAAcgcgc                                                                                                       >  2:175186/1‑149 (MQ=255)
                               cATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATGTGGTCTGGCacc                                                                                                           >  1:890651/1‑142 (MQ=255)
                               cATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAAc                                                                                                           >  1:912465/1‑144 (MQ=255)
                                       aCAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGGCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAgtg                                                                                              <  2:298511/149‑1 (MQ=255)
                                         aaCCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGtt                                                                                            <  2:190421/149‑1 (MQ=255)
                                         aaCCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGtt                                                                                            <  2:870944/149‑1 (MQ=255)
                                          aCCAGGCGGCGAAAGAAGCACAACTGGTCGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGGCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGttt                                                                                           <  2:882834/149‑1 (MQ=255)
                                           ccAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAgt                                                                                               <  1:720870/144‑1 (MQ=255)
                                            cAGGCGGCGAAAGAAGCCCAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGc                                                                                         <  2:1013348/149‑1 (MQ=255)
                                            cAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGc                                                                                         <  2:690903/149‑1 (MQ=255)
                                                    gAAAGAAGCACAACTGGCAGACGCTATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATcgcg                                                                                 >  2:767999/1‑149 (MQ=255)
                                                    gAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATcgcg                                                                                 <  2:15209/149‑1 (MQ=255)
                                                      cagaagCACAACGGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGt                                                                               <  2:127809/148‑1 (MQ=255)
                                                           gCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTCTCa                                                                          >  2:596826/1‑149 (MQ=255)
                                                            cacaACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGtt                                                                              >  1:911589/1‑144 (MQ=255)
                                                                  tGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACCACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCggg                                                                        >  1:589333/1‑144 (MQ=255)
                                                                  gggCAGACGCCACCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACGAAAGCCTGGGGAGTGTTTGCCGATCGCGGTTATCAGGCTGAc                                                                   <  2:249576/148‑1 (MQ=255)
                                                                     cAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGGCGATCGCGGTTATCAGGCTggcggc                                                                >  2:758818/1‑149 (MQ=255)
                                                                           ccATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCt                                                               >  1:425224/1‑144 (MQ=255)
                                                                             aTCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGt                                                        <  2:897637/149‑1 (MQ=255)
                                                                             aTCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGt                                                        <  2:231230/149‑1 (MQ=255)
                                                                               cGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCt                                                           <  1:166860/144‑1 (MQ=255)
                                                                                     agCGGTATACGTTTGCGATCCAGCATTGATTCTCGTCGGGTTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGaa                                                <  2:725331/149‑1 (MQ=255)
                                                                                       cGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGc                                              <  2:594958/149‑1 (MQ=255)
                                                                                       cGGTATACGCTTGCGAGCCAGCATTGATTCTCGTCGGGCGGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCcgc                                                   <  1:172135/144‑1 (MQ=255)
                                                                                           ataCGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGt                                          <  2:862437/149‑1 (MQ=255)
                                                                                                cTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGgc                                     <  2:474074/149‑1 (MQ=255)
                                                                                                 ttGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGgcg                                    >  2:455848/1‑149 (MQ=255)
                                                                                                 ttGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGgcg                                    >  2:867908/1‑149 (MQ=255)
                                                                                                  tGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCCGGCTGACGGCTCGCTGGTGCCGCGCAGCCAGTCa                                        >  1:726485/1‑144 (MQ=255)
                                                                                                   gcgcTCCTGCATTGATTCTCGTCGTGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAg                                       <  1:594942/144‑1 (MQ=255)
                                                                                                          aGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGGGAAGCCAGGCCGGCGCGttgattg                           >  2:174346/1‑149 (MQ=255)
                                                                                                          aGCATTGATTCTCGCCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGtt                                <  1:333197/144‑1 (MQ=255)
                                                                                                              ttGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGaaaa                       <  2:214802/149‑1 (MQ=255)
                                                                                                                 aTTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGaa                         >  1:358580/1‑144 (MQ=255)
                                                                                                                  ttCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACgaa                   >  2:1013373/1‑149 (MQ=255)
                                                                                                                       gtcgGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCATGCGCGTTGATTGAAAACGAAGAACa              <  2:615883/149‑1 (MQ=255)
                                                                                                                        tcgGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGAGCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGACTGAAAACgaag                  >  1:590662/1‑144 (MQ=255)
                                                                                                                           ggCTGGCGGGAAGCGAGATGATTAGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGgcg          >  2:566472/1‑149 (MQ=255)
                                                                                                                             cTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGCTGATTGAAAACGAAGAACAGGCGCt        >  2:878481/1‑149 (MQ=255)
                                                                                                                                gCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACACCGCAAGATTAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGgc     <  2:706166/149‑1 (MQ=255)
                                                                                                                                  gggAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGGTTTAAAACGAAGAACATGCGCTGgcgc   >  2:864030/1‑149 (MQ=255)
                                                                                                                                   ggAAGCGAGATGATTCGTGCAGGCAAGCAATATGATCTGACACCGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGCTTGAAAACGAAGAACAGGCGCCGGCGCa  >  2:916589/1‑149 (MQ=255)
                                                                                                                                        cGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCa  >  1:702904/1‑144 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCA  >  CP009273/740910‑741189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: