Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,419,933 G→T A112D (GCC→GAC)  hisJ ← histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,419,9330GT98.0% 148.5 / NA 50A112D (GCC→GAC) hisJhistidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (34/15);  total (34/16)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATT  >  CP009273/2419790‑2420071
                                                                                                                                               |                                                                                                                                          
cGATTGCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGCCTCCTGGGTGGATCCTCTCAATACGCCTACCCGTTTGCCTTTCAGCGACGCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCg                                                                                                                                       <  2:496009/149‑1 (MQ=255)
           ccTCTTGGTGCCCAATGCTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATCCGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGt                                                                                                                                 <  1:699473/144‑1 (MQ=255)
            cTTTTGGTGCACAACGTCCATTACCGAACGGCTCCTGGGTGGTGCGCTGCAATATGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCg                                                                                                                           <  2:238602/149‑1 (MQ=255)
                 tgTGCCCCACGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGGAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCg                                                                                                                           <  1:350164/143‑1 (MQ=255)
                    gCCCAATGTTCATTACCGTTCGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTg                                                                                                                        <  1:44018/144‑1 (MQ=255)
                     cccAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCt                                                                                                                  >  2:119312/1‑149 (MQ=255)
                         aTGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTAttt                                                                                                              >  2:742154/1‑149 (MQ=255)
                          tGTTCATTACCGAACGTCTCCTGGGAGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTc                                                                                                             >  2:533918/1‑149 (MQ=255)
                              cATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTAttt                                                                                                              >  1:837568/1‑144 (MQ=255)
                               aTTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCGGAATGTCAGAATTTTTCGCAACCACAAAACAAGAATCGTCGCCATACAGTTGGTCGGTGAAAGCTGTTTCTTGcg                                                                                                        >  2:907127/1‑148 (MQ=255)
                                   ccGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGc                                                                                                         <  1:299443/144‑1 (MQ=255)
                                        cGTCTCCTGGGTGGCGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGttttt                                                                                               <  2:967843/149‑1 (MQ=255)
                                        cGTCTCCTGGCTGGTGCCCCGCAATACGCCTACCCGTTTGCCTTTCCGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCCCCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGttttt                                                                                               <  2:884434/149‑1 (MQ=255)
                                              cTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTGAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACGGTATGTCGGTGAAGGCGATTTCTTGCTGACGTTTTTc                                                                                              >  1:722158/1‑144 (MQ=255)
                                                gggTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCg                                                                                            <  1:675431/144‑1 (MQ=255)
                                                          cTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCCGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTGTTGCTGACGTTTTTCCGTAATGGAAAg                                                                                  >  1:3227/1‑144 (MQ=255)
                                                          cTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTACCGTAATGGAAAGCGATg                                                                             >  2:836514/1‑149 (MQ=255)
                                                                 aCGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACatgtt                                                                      >  2:486130/1‑147 (MQ=255)
                                                                   gCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAGCGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATgg                                                                    >  2:324070/1‑149 (MQ=255)
                                                                       aCCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTGTTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTc                                                                >  2:340258/1‑149 (MQ=255)
                                                                        cccGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGATTTTCCGTAACGGAAAGCGTTGACAGGATgg                                                                    >  1:507231/1‑144 (MQ=255)
                                                                        cccGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAAACGTCAGCGTACAGTTTGTCGGGGAAGGCGATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGCCATGATGGCGGCa                                                               >  2:189816/1‑149 (MQ=255)
                                                                         ccGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGGCaa                                                              >  2:826259/1‑149 (MQ=255)
                                                                           gtgtGCCTTTCATCTCCCCGCCTGTCGGCTGAATGTCAGACTTTTTCGCCCCCACCCAACGCGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTTACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGt                                                                 <  1:594914/141‑1 (MQ=255)
                                                                           gTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGGTTCAAtc                                                            >  2:262390/1‑149 (MQ=255)
                                                                               gCCTTTCAGCGACTCGACTGTCGGCTGAATATAAGATTATTGCTCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAAtcttct                                                        >  2:1019149/1‑149 (MQ=255)
                                                                                 cTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGCAAAGCGATGACATGATGGCGTCAAtct                                                           >  1:785467/1‑144 (MQ=255)
                                                                                  tttCAGCGACTCGACTGTCGGCTGGATGTCAGAAGTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCg                                                     >  2:523345/1‑149 (MQ=255)
                                                                                       gCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCg                                                     >  1:648712/1‑144 (MQ=255)
                                                                                        cgactcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGc                                                    >  1:123752/1‑144 (MQ=255)
                                                                                         gactcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCt                                                   >  1:709219/1‑144 (MQ=255)
                                                                                          actcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGCTGGCTTGTTGGCGTCAATCTCTTTCGCTTTTagg                                             >  2:584668/1‑147 (MQ=255)
                                                                                          actcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAg                                             >  2:1028175/1‑149 (MQ=255)
                                                                                             cgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGAc                                          <  2:1022904/149‑1 (MQ=255)
                                                                                              gactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTaa                                              <  1:468778/144‑1 (MQ=255)
                                                                                              gactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACg                                         >  2:528737/1‑149 (MQ=255)
                                                                                                   tCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAGTCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACg                                         >  1:203633/1‑144 (MQ=255)
                                                                                                      gCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTCTTAAGGACGGGa                                      >  1:147431/1‑144 (MQ=255)
                                                                                                             gTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACCTGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGc                               >  1:262707/1‑144 (MQ=255)
                                                                                                             gTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGGTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGc                               <  1:699909/144‑1 (MQ=255)
                                                                                                              tCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACgga                              >  1:214890/1‑142 (MQ=255)
                                                                                                               cAGAATTTTTCGGCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGAGATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGc                        >  2:743674/1‑149 (MQ=255)
                                                                                                                    tttttCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGAtt                   <  2:43022/149‑1 (MQ=255)
                                                                                                                        tCGCCACCCCCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCg               <  2:351227/149‑1 (MQ=255)
                                                                                                                               caccaAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCgggtcgtcggc             >  1:752343/1‑135 (MQ=255)
                                                                                                                                   aaaCGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAGAc         >  1:809713/1‑144 (MQ=255)
                                                                                                                                    aaCGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGCCGGGATTAACGCCTCCAGCGGATTTTCGACAAACGTACAt   >  2:723818/1‑149 (MQ=255)
                                                                                                                                    aaCGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGGTTAACGCATCCAGCGGCTGTTCGACAAACGTACAt   >  2:828904/1‑149 (MQ=255)
                                                                                                                                     aCGAGAATCGGCAGCGGACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACAtt  <  2:912678/149‑1 (MQ=255)
                                                                                                                                       gagaATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCCCACTTCCGACAAACGTAc     >  1:370908/1‑144 (MQ=255)
                                                                                                                                               |                                                                                                                                          
CGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATT  >  CP009273/2419790‑2420071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑