Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,420,328 2 bp→AA intergenic (‑61/+159) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,420,3280GA100.0% 153.2 / NA 46intergenic (‑61/+160)hisJ/argThistidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)
*CP0092732,420,3290CA100.0% 148.4 / NA 46intergenic (‑62/+159)hisJ/argThistidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/23);  total (23/23)

TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA‑‑‑‑AATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCA  >  CP009273/2420188‑2420465
                                                                                                                                            ||                                                                                                                                            
ttttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaa                                                                                                                                       <  2:412284‑M1/149‑3 (MQ=255)
ttttGCGGAACCGCAGCAAACGCGGCAGTTGCGCTGGAGAAGGCCATAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaa                                                                                                                                       <  2:3831‑M1/149‑3 (MQ=255)
              agcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatca                                                                                                                         >  2:589174‑M1/1‑133 (MQ=255)
                caAACGCAGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTAAACTTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaag                                                                                                                       <  2:1029492‑M1/149‑19 (MQ=255)
                     gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcacggcaaa                                                                                                                  >  2:259076‑M1/1‑126 (MQ=255)
                          aGTTGCGCTGGATGAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatc                                                                                                             <  2:815267‑M1/149‑29 (MQ=255)
                                 cTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgcccc                                                                                                      >  2:599737‑M1/1‑114 (MQ=255)
                                    gagaAGGCCAGAAACAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTTTTTTCAGCTGACAGACGCTTTATAGGTAATATTTTGAGGTTTGTTACACGTCTCGTAACATTACATTAATTGACCAGCAAGGCACGATATgccgcc                                                                                                        >  1:569078/1‑144 (MQ=255)
                                        aGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagac                                                                                               <  2:780472‑M1/149‑43 (MQ=255)
                                          gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgtta                                                                                                  <  1:236810‑M1/144‑40 (MQ=255)
                                           ccAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgta                                                                                            <  2:883406‑M1/149‑46 (MQ=255)
                                            cAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttaaa                                                                                                >  1:15420‑M1/1‑103 (MQ=255)
                                                 ccAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattattt                                                                                      >  2:946353‑M1/1‑98 (MQ=255)
                                                           cGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTCTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcat                                                                                 >  1:201235‑M1/1‑88 (MQ=255)
                                                            gATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcatt                                                                                <  1:380648‑M1/144‑58 (MQ=255)
                                                             aTAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTTTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaat                                                                          <  2:846249‑M1/149‑64 (MQ=255)
                                                                  aCCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATGTTTACAGGTTTGTAACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatattccgccccgttagacgtattattttgcattaaaaatgctta                                                                     >  2:971736‑M1/1‑81 (MQ=255)
                                                                            tCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgacaatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtat                                                                <  1:333059‑M1/144‑74 (MQ=255)
                                                                              aTAATGTCCTCAACTGACAGTCTTTGTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttc                                                         >  2:533718‑M1/1‑69 (MQ=255)
                                                                                aaTGTCCTCACCTGACAGTCTTTTTAAAGGAAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaa                                                       <  2:17472‑M1/149‑83 (MQ=255)
                                                                                aaTGTCCTCAACGTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcat                                                            <  1:536418‑M1/144‑78 (MQ=255)
                                                                                    tCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgca                                                   >  2:41680‑M1/1‑63 (MQ=255)
                                                                                    tCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttca                                                        >  1:934477‑M1/1‑63 (MQ=255)
                                                                                    tCCTCAACTGACAGTATTTTTATAGGGAATATTTCCAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttca                                                        <  1:473813‑M1/144‑82 (MQ=255)
                                                                                     ccTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcac                                                  <  2:639227‑M1/149‑88 (MQ=255)
                                                                                       tCAACTGACAGTCTTTTTATAGGGAATACAACAAGAGAAGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcacca                                                >  2:1020430‑M1/1‑60 (MQ=255)
                                                                                         aaCTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcacaccg                                              >  2:477908‑M1/1‑58 (MQ=255)
                                                                                            tGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaaggcttaattatgcatttcaatcaaccacgata                                           >  2:51274‑M1/1‑55 (MQ=255)
                                                                                               cAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtg                                        <  2:68270‑M1/149‑98 (MQ=255)
                                                                                               cAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtg                                        >  2:368396‑M1/1‑52 (MQ=255)
                                                                                                 ttcTTTTGATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaactaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgata                                           <  1:181817‑M1/143‑95 (MQ=255)
                                                                                                 gTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTAGCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgca                                      <  2:810206‑M1/149‑100 (MQ=255)
                                                                                                    tttttAGAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttaattaaaaatgcttaagtatgcatttcaatgcaccacgatagtg                                        >  1:941215‑M1/1‑47 (MQ=255)
                                                                                                      tttATATGTAATATTTACAGGTTTGTTCCACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaa                                 <  2:670966‑M1/149‑105 (MQ=255)
                                                                                                       ttATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaa                                     <  1:216920‑M1/144‑101 (MQ=255)
                                                                                                           aGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaa                                 <  1:478500‑M1/144‑105 (MQ=255)
                                                                                                             ggAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcg                          >  2:197114‑M1/1‑38 (MQ=255)
                                                                                                             ggAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgtcagacgtattatttttcattaaaaatacttaagtatgcatttcaatgcaccacgaaagtgcaacaaacc                               >  1:238592‑M1/1‑38 (MQ=255)
                                                                                                                          gTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatga             >  2:226501‑M1/1‑25 (MQ=255)
                                                                                                                               ttACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcatt        <  2:84499‑M1/149‑130 (MQ=255)
                                                                                                                               ttACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatga             >  1:723966‑M1/1‑20 (MQ=255)
                                                                                                                                tACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaagtgcttgagtatacagttcaatgcaccacgatagggcaacaaacctggcgtgcaccatgatgatcattc       <  2:406121‑M1/149‑131 (MQ=255)
                                                                                                                                 acacTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatc           <  1:530876‑M1/144‑127 (MQ=255)
                                                                                                                                     ttATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcattccatca  >  2:329483‑M1/1‑14 (MQ=255)
                                                                                                                                     ttATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcattccatca  >  2:607728‑M1/1‑14 (MQ=255)
                                                                                                                                     ttATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcattccatca  >  2:775278‑M1/1‑14 (MQ=255)
                                                                                                                                            ||                                                                                                                                            
TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA‑‑‑‑AATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCA  >  CP009273/2420188‑2420465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: