Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 148,861 G→C R147G (CGC→GGC)  yadM ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273148,8610GC98.5% 225.4 / NA 67R147G (CGC→GGC) yadMputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (29/37);  minor base T (0/1);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGATGATGCATTTCC  >  CP009273/148718‑149005
                                                                                                                                               |                                                                                                                                                
taaccaTCCTTCTTATTGATCGATAAAAGTCAACGTCGCCCGTGCACGAAAATTCCCCGGTACGCCTTGCCCTCCATCCGTTCCACGTAGCGCAACGCTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCt                                                                                                                                             <  2:371954/144‑1 (MQ=255)
    tATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTACCCTGCGTCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTc                                                                                                                                         >  2:160686/1‑149 (MQ=255)
         ttcttATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTCTCTTTTCCGCAc                                                                                                                                    >  2:363497/1‑149 (MQ=255)
              aTTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTAGTCTGTGCTCCAGCCTATCTTTTCCGCCCTg                                                                                                                                  >  1:818685/1‑146 (MQ=255)
                  aTAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTACAGCCTATCTTTTCCGCACTGTTAGCtt                                                                                                                           >  2:678425/1‑149 (MQ=255)
                    aGATGAAATTAAACGTCCCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTa                                                                                                                         <  2:418626/149‑1 (MQ=255)
                     gATGAAAGTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGGCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTaa                                                                                                                        <  2:108220/149‑1 (MQ=255)
                      aTGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGCCCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTCTCTTTTCCGCCCTGTTAGGttt                                                                                                                          >  1:97800/1‑146 (MQ=255)
                        gAAATTAACCGTCGCCAGTGCACGAAAATGCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCAATTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCATCCTATCTTTTCCGCACTGTTAGGTTTaa                                                                                                                        <  1:582532/146‑1 (MQ=255)
                        gAAATTAAACGTCGCCAGTGCACGAAAATTCCCTGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGa                                                                                                                     >  2:412817/1‑149 (MQ=255)
                         aaaTTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTGCACGTAGCGCAACGGTCATTTCATGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTCCGCACTGTTAGGTTTAAGGaaa                                                                                                                   >  2:469853/1‑149 (MQ=255)
                           ctcaaactgccccAGTGCACCAAAATCCCCCGGTCCGCCTTGCCCTGCATCTGTATCACGTACCGCAACGGTCATTTAAAGTCACTTAGCGGGCTGGATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGa                                                                                                                     <  1:118787/135‑1 (MQ=255)
                                aCGGCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGTGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGCCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTg                                                                                                                <  1:501115/146‑1 (MQ=255)
                                 cGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTgg                                                                                                               <  1:557369/146‑1 (MQ=255)
                                     gctaGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCatcatc                                                                                                        <  2:129642/146‑1 (MQ=255)
                                       cAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCag                                                                                                      <  2:723452/149‑1 (MQ=255)
                                       cAGTGCACCAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCag                                                                                                      <  2:24836/149‑1 (MQ=255)
                                          tGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCagtag                                                                                                   <  2:935653/149‑1 (MQ=255)
                                          tGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCag                                                                                                      <  1:487025/146‑1 (MQ=255)
                                            cACGAAAATTCCCCGGTCCGCCTTGCCCTGCATCTGTTTCACGTAGTGCAACGGTCATTACAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCagta                                                                                                    <  1:714673/146‑1 (MQ=255)
                                            cACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCagta                                                                                                    <  1:61868/146‑1 (MQ=255)
                                               gAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCgg                                                                                              >  2:90547/1‑148 (MQ=255)
                                               gAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGt                                                                                              >  2:140289/1‑149 (MQ=255)
                                               gAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATAATAAGTAGTc                                                                                                 >  1:235406/1‑146 (MQ=255)
                                                    ttCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTCTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGtttttt                                                                                         <  2:789122/149‑1 (MQ=255)
                                                       cccGGTACGCCTTGCCCTGCATCTGTTTGACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTCGTCCGTTTTTTGaa                                                                                      >  2:901496/1‑149 (MQ=255)
                                                       cccGGTACGCCTTGCCCGGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGtttttt                                                                                         <  1:127939/146‑1 (MQ=255)
                                                        ccGGTACGCCTTGCCCGGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTCTCGGGCGGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGaaa                                                                                     <  2:552693/149‑1 (MQ=255)
                                                          ggTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGaa                                                                                      >  1:1043180/1‑146 (MQ=255)
                                                               gCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCCTAc                                                                              >  2:103710/1‑149 (MQ=255)
                                                                     cccTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGatat                                                                        <  2:201390/149‑1 (MQ=255)
                                                                     cccTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGatat                                                                        <  2:1054503/149‑1 (MQ=255)
                                                                     cccTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGatat                                                                        <  2:941545/149‑1 (MQ=255)
                                                                           aTCGGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTg                                                                  <  2:117856/149‑1 (MQ=255)
                                                                            tCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTgt                                                                 <  2:878178/149‑1 (MQ=255)
                                                                                 ttCACGTAGCGCAACGGTCTTTCCAAGCCCCTCATCGGGCTGCACCTCGTCTGTTCTCCAGCCTATCTTTTCCGCCCTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGtcgtcg                                                            <  2:496517/149‑1 (MQ=255)
                                                                                 ttCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGATGCTTTTTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGtcgtcg                                                            <  2:550514/149‑1 (MQ=255)
                                                                                  tCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTgtcgt                                                              <  1:289122/146‑1 (MQ=255)
                                                                                   cACGTAGCGCAACGGGCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGaa                                                          <  2:210139/149‑1 (MQ=255)
                                                                                        aGCGCACCGGTCATGTCAAGACCCTTATCGGGCGGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAATGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGa                                                        <  1:208885/146‑1 (MQ=255)
                                                                                        aGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGa                                                     <  2:895673/149‑1 (MQ=255)
                                                                                             aaCGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCTATAGTACTTGTCGTCGAAGATGAATCAc                                                <  2:856728/149‑1 (MQ=255)
                                                                                                ggTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAg                                             >  2:678734/1‑149 (MQ=255)
                                                                                                ggTCATTTCAAGACCCGTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCAc                                                <  1:597016/146‑1 (MQ=255)
                                                                                                    aTTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATAACCAGACTg                                         >  2:425427/1‑149 (MQ=255)
                                                                                                     tttCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGTATCTCTTTCCGATATTACTTGCCGGCGAAGCTAAAACACCAGAc                                           >  1:819096/1‑146 (MQ=255)
                                                                                                     tttCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACAAATACCGATATTACGTGTCGTCGAAGATGAATCACCAGACTGc                                        >  2:213946/1‑149 (MQ=255)
                                                                                                      ttCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGTTACTTATACAGATCTGAGTTGTCGTCGGAGATGAAtagcgggact                                          >  1:711438/1‑137 (MQ=255)
                                                                                                        cAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTACAGCCTCTCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCGGTAGTCCGTTTTTTGAAACCCATACCGGTATTACTTGTCGCCGAAGATGAAACACCAGACtcc                                        >  1:269654/1‑144 (MQ=255)
                                                                                                         aaGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCg                                       <  1:972397/146‑1 (MQ=255)
                                                                                                           gACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTATTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGt                                     <  1:605921/146‑1 (MQ=255)
                                                                                                                         tGCGTCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAgg                       <  1:286800/146‑1 (MQ=255)
                                                                                                                           cATCTCGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTCGAAGGAAAGTGGCATCCTCAGCAGGCCGTTGTGTTTATTCTTTACCGATATTACTAGTCGTAGAAGATGAATCACCAGACTGCGGTATAATAAGCTTCGGTg                     >  1:737849/1‑146 (MQ=255)
                                                                                                                             tctcGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAg                   >  1:595522/1‑146 (MQ=255)
                                                                                                                             tctcGTCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCtt                >  2:202703/1‑149 (MQ=255)
                                                                                                                              ctctTCTGCGCGCCAGTCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTtg               <  2:427833/149‑1 (MQ=255)
                                                                                                                                  tCTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTtgatga           >  2:977180/1‑149 (MQ=255)
                                                                                                                                   cTGTGCTCCAGCCTATCTTTTCCGCACTGTTAGTTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTgatgat          <  2:202819/149‑1 (MQ=255)
                                                                                                                                    ggtgCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGatgatg         <  2:1108783/148‑1 (MQ=255)
                                                                                                                                     gtgCTCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGATGATGc        >  2:1103279/1‑149 (MQ=255)
                                                                                                                                     ctgCCCCAGTCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTtgatga           <  1:753598/145‑1 (MQ=255)
                                                                                                                                         tCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGGGTCACTAGAATGAGGTATAATAAGCTTAGGTGAGCTTGATGATGCAttt    >  2:154216/1‑149 (MQ=255)
                                                                                                                                         tCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGATGATTCAttt    >  2:301457/1‑149 (MQ=255)
                                                                                                                                         tCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGATGATGCa       >  1:202349/1‑146 (MQ=255)
                                                                                                                                         tCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGATGATGCa       >  1:985358/1‑146 (MQ=255)
                                                                                                                                         tCCAGCCTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGATGATGCAttt    >  2:300399/1‑149 (MQ=255)
                                                                                                                                           cAGCCTATCTTTTCCGCACTGCTATGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGATGATGCATTTcc  <  2:804094/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCGAAGATGAATCACCAGACTGCGGTATAATAAGCTTAGGTGAGCTTGATGATGCATTTCC  >  CP009273/148718‑149005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: