Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,178,277 G→T E27* (GAA→TAA)  yqiC → BMFP family putative fusogenic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,178,2770GT98.7% 236.3 / NA 76E27* (GAA→TAA) yqiCBMFP family putative fusogenic protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (40/35);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGA  >  CP009273/3178134‑3178422
                                                                                                                                               |                                                                                                                                                 
ggTTTATTACTTACCCTTATCCCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGAt                                                                                                                                              <  2:1005063/149‑1 (MQ=255)
 gTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAg                                                                                                                                                >  1:480640/1‑146 (MQ=255)
    tATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTgg                                                                                                                                          <  2:876069/149‑1 (MQ=255)
     aTGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATAAGGGAGTTCGGGTAAGATGTGga                                                                                                                                         <  2:617818/149‑1 (MQ=255)
       gACGTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGCCCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTgg                                                                                                                                          <  1:56958/146‑1 (MQ=255)
          ttACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGaaaa                                                                                                                                    <  2:139508/149‑1 (MQ=255)
          ttACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGaaaa                                                                                                                                    <  2:487306/149‑1 (MQ=255)
              ccTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGaaaaa                                                                                                                                   >  1:827355/1‑146 (MQ=255)
               cTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATCCCTAAAGGAATCAGGGAGGTCGGGTAAGATGAGGAGaaaaga                                                                                                                                  >  1:563567/1‑144 (MQ=255)
                ttATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGaaaaaaa                                                                                                                                 >  1:649557/1‑146 (MQ=255)
                ttATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAAGCAGGGAGTTCGGGTAAGACATGGAGaaaaaaa                                                                                                                                 >  1:105990/1‑146 (MQ=255)
                  aTCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGc                                                                                                                            >  2:838601/1‑149 (MQ=255)
                    cGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGTAGAAACAAATCCg                                                                                                                             <  1:552319/146‑1 (MQ=255)
                     gCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCaa                                                                                                                         >  2:442613/1‑149 (MQ=255)
                      cACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGcc                                                                                                                           >  1:348615/1‑146 (MQ=255)
                      cACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCaaa                                                                                                                        >  2:1021169/1‑149 (MQ=255)
                              tGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTa                                                                                                                   >  1:656841/1‑146 (MQ=255)
                                gCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGATTCACGAATCAATGCCTAAAGGAATCAGGGAATTCGGGTAAGATGTGGAGAAAAAAATCCGACAAACCCTACAAgc                                                                                                              >  2:307427/1‑149 (MQ=255)
                                 cACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACaa                                                                                                                >  1:751934/1‑146 (MQ=255)
                                     cAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGGTCGGGTGAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAgcgc                                                                                                            >  1:447601/1‑146 (MQ=255)
                                      aaCACCTATCAGTACCGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCt                                                                                                        <  2:691262/149‑1 (MQ=255)
                                      aaCACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCa                                                                                                           <  1:1026148/146‑1 (MQ=255)
                                       acacCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAg                                                                                                          >  1:50545/1‑146 (MQ=255)
                                       acacCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTg                                                                                                       >  2:196270/1‑149 (MQ=255)
                                         acCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAc                                                                                                     >  2:687886/1‑149 (MQ=255)
                                           cTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAcgc                                                                                                   >  2:707274/1‑149 (MQ=255)
                                              tCAGTACAGGGAAACTCGTATGATTGACCCGAAAAAAATTGAGGAAATCGCTCGCCAGGTTAAAGTACCAAAGCCGAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAcgc                                                                                                   >  1:1011515/1‑146 (MQ=255)
                                              acAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAACCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCCGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGcc                                                                                                <  2:107019/148‑1 (MQ=255)
                                                aGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACgcgc                                                                                                 >  1:460264/1‑146 (MQ=255)
                                                  tccAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGa                                                                                            <  2:249345/147‑1 (MQ=255)
                                                  tACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCGCGAATCAATGCCTAAAGGAATGAGGGAGTTCGGGTGGGATGTGAGAAAAAAATCCGCCGACCCCTCCAAGCGCAGCTGACGCNCCTc                                                                                              >  1:840821/1‑146 (MQ=255)
                                                   aCAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                                                                                           <  2:882744/149‑1 (MQ=255)
                                                     aGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAACAATCCGCCAAACCCTACAAGCGCAGCTGACGCACCTCGAACt                                                                                         >  2:677729/1‑149 (MQ=255)
                                                     aGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCGGACGCGCCTCGACCt                                                                                         >  2:537299/1‑149 (MQ=255)
                                                      gcgtaaCTATGATGATTGTCCCGACAAAAATTGAGCAAATCGCTGGCAAGGTTCCCGAGTCAATGCCTAATGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAATCCCTACAAGCGCAGCTGACGCGCCTCGAc                                                                                           <  1:344440/142‑1 (MQ=255)
                                                        gAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCt                                                                                         >  1:617608/1‑146 (MQ=255)
                                                            cTACAGTGATTGACCCGAAAAAAAGTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTa                                                                                     <  1:255057/146‑1 (MQ=255)
                                                             tACAATGATTGACCAGAAATAAATTGAGCAAATCACTCGCCAGGTTCACGAATCGATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAACATCCGCCTAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTaa                                                                                    <  1:783876/146‑1 (MQ=255)
                                                                aaTGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGAGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGcc                                                                                 <  1:989032/146‑1 (MQ=255)
                                                                        aCCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTc                                                                      <  2:935480/149‑1 (MQ=255)
                                                                        aCCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTc                                                                      <  2:935573/149‑1 (MQ=255)
                                                                        aCCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTc                                                                      >  2:185815/1‑149 (MQ=255)
                                                                         cccGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCg                                                                     >  2:294474/1‑149 (MQ=255)
                                                                          ccGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGGAAGCCGTGAAGCGtt                                                                       >  1:111777/1‑146 (MQ=255)
                                                                           ggAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGAc                                                                   <  2:825604/148‑1 (MQ=255)
                                                                           cGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAAGCGCCGCGACCTTGTAAGCCGTGAAGAGTTCGAc                                                                   >  2:712292/1‑149 (MQ=255)
                                                                            gAAAAAAATTGAGCAAACCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCg                                                                     <  1:884434/146‑1 (MQ=255)
                                                                                  aaTTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGTCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCTAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTAGTAAGCCGTGAAGAGTTCGACGTCCaaa                                                            <  2:747152/149‑1 (MQ=255)
                                                                                  aaTTGAGCAAATCGCCCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCaaa                                                            <  2:98880/149‑1 (MQ=255)
                                                                                   aTTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAAc                                                           <  2:631381/149‑1 (MQ=255)
                                                                                        gCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGc                                                         <  1:440543/146‑1 (MQ=255)
                                                                                         cAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCa                                                        <  1:1734/146‑1 (MQ=255)
                                                                                         cAAATCGCACGCCAGGTTCACGAATCAAGGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGt                                                     <  2:998035/149‑1 (MQ=255)
                                                                                          aaaTCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCaa                                                       <  1:158490/146‑1 (MQ=255)
                                                                                                ctcgcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTg                                                 >  1:940025/1‑146 (MQ=255)
                                                                                                 tcgcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGt                                                >  1:45113/1‑146 (MQ=255)
                                                                                                  cgcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACg                                            <  2:293940/149‑1 (MQ=255)
                                                                                                  cgcCAGGTCCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACg                                            <  2:385621/149‑1 (MQ=255)
                                                                                                       ggTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGa                                          <  1:764657/146‑1 (MQ=255)
                                                                                                        gTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGCAAAAAAAATCCGCCAAACCCGACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCCACACCTGATACGGAc                                         >  1:685037/1‑146 (MQ=255)
                                                                                                        gTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGAc                                         >  1:1012653/1‑146 (MQ=255)
                                                                                                         ttCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACt                                        >  1:1033648/1‑146 (MQ=255)
                                                                                                          tCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTCCGGACTCggg                                    >  2:195645/1‑147 (MQ=255)
                                                                                                          tCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCGTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTg                                    <  2:1098780/149‑1 (MQ=255)
                                                                                                           cACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGa                                   <  2:714180/149‑1 (MQ=255)
                                                                                                            aCGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGaa                                  >  2:1049508/1‑149 (MQ=255)
                                                                                                                    aTGCCTAAAGGAATCAGGGAGTTCGGGTACGATGTGGAGAAAAAAATCCGCCAGACCCTACGAGCGCGGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAAGGCAAGTCCTGTTACGGACACGTGAAAAACTGgc                          >  2:700402/1‑149 (MQ=255)
                                                                                                                     tGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAGATGCGCCCAACCCTCGAAGCGTAGGTGACTCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTg                            >  1:1012093/1‑146 (MQ=255)
                                                                                                                      gCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTgg                           >  1:751888/1‑146 (MQ=255)
                                                                                                                           aaGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCtt                   >  2:1030636/1‑149 (MQ=255)
                                                                                                                            aGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTg                  <  2:211140/149‑1 (MQ=255)
                                                                                                                                 tCAGGGAGTTCTGGTAAGATGGAGAGAAAAAACTCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTACTTGaa                <  1:732721/146‑1 (MQ=255)
                                                                                                                                  cAGGGAGTTCGGTGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACaaa            <  2:37226/149‑1 (MQ=255)
                                                                                                                                     ggAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTGGAACAAAGaa         >  2:202001/1‑149 (MQ=255)
                                                                                                                                     ggAGTTCGGGTAAGATGTGGAGAAAAAAATCAAACAAACCCTACAAGCGCAGCTGACGCGACTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCGTGAACaaa            >  1:741396/1‑146 (MQ=255)
                                                                                                                                        gTTCGGGTAAGATGTGGAGAAAAAACTCCGCCAAGCCCTCCAGGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGaa         <  1:838727/146‑1 (MQ=255)
                                                                                                                                            gggtAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAACCGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATAAGCGa  >  2:41645/1‑149 (MQ=255)
                                                                                                                                            gggtAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGa  <  2:69438/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGA  >  CP009273/3178134‑3178422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: