Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,049,665 A→C I12S (ATC→AGC)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,049,6650AC97.7% 257.4 / ‑4.9 87I12S (ATC→AGC) glnLsensory histidine kinase in two‑component regulatory system with GlnG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (40/44);  minor base T (0/2);  total (41/46)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGG  >  CP009273/4049520‑4049810
                                                                                                                                                  |                                                                                                                                                 
tGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGctct                                                                                                                                                <  2:729093/149‑1 (MQ=255)
  agaAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGc                                                                                                                                              >  2:42106/1‑149 (MQ=255)
   gaAGTAGCGCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGctct                                                                                                                                                >  1:925811/1‑146 (MQ=255)
    aaGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGCGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTTATGGATGGCCCGGTTGTCATCGATTAACAAAATATTGTTAATCAGCGAGTTGAGGGTCTg                                                                                                                                               >  1:326862/1‑146 (MQ=255)
    aaGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGccc                                                                                                                                            >  2:235276/1‑149 (MQ=255)
      gTAGCTCAATAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGcc                                                                                                                                             <  1:755015/146‑1 (MQ=255)
      gTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTGGAGGCGCTGCCCAg                                                                                                                                          >  2:656794/1‑149 (MQ=255)
       tAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGccc                                                                                                                                            <  1:306474/146‑1 (MQ=255)
       tAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGCCACCTTTTTGTTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGc                                                                                                                                         >  2:1122628/1‑149 (MQ=255)
        aGCTCCACAGTTCCGGGAACGGTGTACCAAACCATTTGCGGGAGCTTGGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAACGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCa                                                                                                                                           <  1:498420/146‑1 (MQ=255)
         gcgcAACAGTGCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAg                                                                                                                                          <  1:36629/143‑1 (MQ=255)
            cAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCAt                                                                                                                                       >  1:249302/1‑146 (MQ=255)
            cAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCAt                                                                                                                                       <  1:158905/146‑1 (MQ=255)
            cAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCAt                                                                                                                                       >  1:494297/1‑146 (MQ=255)
             aaCAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCggg                                                                                                                                   <  2:866196/149‑1 (MQ=255)
                 gTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCGCTGCCCAGCATCGGGc                                                                                                                                  >  1:409414/1‑146 (MQ=255)
                    ccGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGc                                                                                                                               <  1:157139/146‑1 (MQ=255)
                      ggTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGt                                                                                                                             >  1:832850/1‑146 (MQ=255)
                             gtgtACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGGTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCCGtt                                                                                                                      >  1:412325/1‑146 (MQ=255)
                              tgtACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAGTCAGCGAGTTGAGGCTCTGCCAAGGATCGGGCTGCGTGCCTGTTg                                                                                                                     >  1:512731/1‑146 (MQ=255)
                               gtACCAATCAATTTGCGGGAGCTTTGGGCGACCAGTTGTTGCGCGGCAGGGTTGGCGTTATGGCTTGTTAAGTTGGCATCGATAAGTTAAACACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCAt                                                                                                                 <  2:555952/149‑1 (MQ=255)
                               gtACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGc                                                                                                                    <  1:889449/146‑1 (MQ=255)
                               gtACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGc                                                                                                                    <  1:889486/146‑1 (MQ=255)
                                 aCCAAACAATGTGCGGGAGCTTGGGGCAAGTACTTGTTGCGCGGCAGGGTAGGCGGAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATaa                                                                                                               <  2:61322/149‑1 (MQ=255)
                                     aaCAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATaaa                                                                                                              >  1:416347/1‑146 (MQ=255)
                                     aaCAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCa                                                                                                           >  2:536087/1‑149 (MQ=255)
                                           ttGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATAAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGtc                                                                                                        <  1:959606/146‑1 (MQ=255)
                                               gggAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGaa                                                                                                 >  2:810717/1‑149 (MQ=255)
                                               gggAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGaa                                                                                                 >  2:810421/1‑149 (MQ=255)
                                                  aGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCCCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATCAAGCAGTCTCCTGAACAg                                                                                              >  2:1091138/1‑149 (MQ=255)
                                                     tttGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTAATGGATTAACAAAATAATGTTAATCGGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATACAGCAGTCTCCTGAACCGGtt                                                                                           >  2:384221/1‑149 (MQ=255)
                                                        gggCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGtt                                                                                           <  1:165632/146‑1 (MQ=255)
                                                         ggCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTg                                                                                          <  1:211458/146‑1 (MQ=255)
                                                         ggCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGACCCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCAc                                                                                       <  2:1019571/149‑1 (MQ=255)
                                                          gCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGc                                                                                         >  1:708369/1‑146 (MQ=255)
                                                             aGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCAcc                                                                                      >  1:903690/1‑146 (MQ=255)
                                                                     ttgCGCGGCATGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGt                                                                              <  1:2626/146‑1 (MQ=255)
                                                                     tggcgcGGGAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGt                                                                              <  1:217616/144‑1 (MQ=255)
                                                                     ctgCCCGGCAGGGTTGGCCTAAGGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATTCAGGGTGCTCCTGAACAGGTTGCACCATTTTAGt                                                                              <  1:743027/145‑1 (MQ=255)
                                                                      tgCGCGGCAAGGTTGGCGTAATAGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCAt                                                                          <  2:81150/149‑1 (MQ=255)
                                                                       gcgcGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGc                                                                            <  1:972048/146‑1 (MQ=255)
                                                                        cgcgGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCa                                                                           >  1:1046879/1‑146 (MQ=255)
                                                                        cgcgGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCACCGGGCTGCGTGCCTGTTGCCATAAATCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATta                                                                        <  2:745797/149‑1 (MQ=255)
                                                                        cgcgGCAGGGGTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCa                                                                           <  1:806653/146‑1 (MQ=255)
                                                                         gcgGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGCGCTGCGCGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCAt                                                                          <  1:874255/146‑1 (MQ=255)
                                                                         gcgACAGGGATGGCATAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCGCTGCCCAGCATCGGGCTGCGTGCCTGTTCCCATAAAGCAGTCTCCGGAACAGGGTGCACCACTTTCGTGCag                                                                          >  1:910833/1‑145 (MQ=255)
                                                                                ggTTGGCGTAATGCATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGCGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCt                                                                   <  1:541692/146‑1 (MQ=255)
                                                                                     gCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTa                                                              <  1:545737/146‑1 (MQ=255)
                                                                                      cGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGa                                                          >  2:283561/1‑149 (MQ=255)
                                                                                           tGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTCTTTACGGATa                                                        >  1:775453/1‑146 (MQ=255)
                                                                                            ggATCGCCAGGTTGTTATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATaaaaa                                                    <  2:398811/149‑1 (MQ=255)
                                                                                            ggATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATaaaaa                                                    <  2:462720/149‑1 (MQ=255)
                                                                                             gATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATaac                                                      >  1:90989/1‑145 (MQ=255)
                                                                                              aTCGCCAGGTTGTCATTGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATaaaa                                                     >  1:735122/1‑146 (MQ=255)
                                                                                              aTCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGAATTATAGCTTTTTATGGAGaaaa                                                     >  1:675012/1‑146 (MQ=255)
                                                                                              aTCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCGGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTGTTTACGGATAAAAAgc                                                  <  2:276499/149‑1 (MQ=255)
                                                                                               tCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTGGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATaaaaa                                                    <  1:778120/146‑1 (MQ=255)
                                                                                                  ccAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAAAAGGTTGCACCATTTTAGTGAATTATAGCTTTTTACGGATAAAAAgcg                                                 >  1:105851/1‑146 (MQ=255)
                                                                                                   cAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGaa                                             <  2:200488/149‑1 (MQ=255)
                                                                                                       ttGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCAt                                         <  2:1085726/149‑1 (MQ=255)
                                                                                                           tCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCAt                                         <  1:1034391/146‑1 (MQ=255)
                                                                                                           tCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGATGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCag                                      >  2:60009/1‑149 (MQ=255)
                                                                                                            cATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACATGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCaga                                     <  2:254965/149‑1 (MQ=255)
                                                                                                             aTCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCagag                                    >  2:337767/1‑149 (MQ=255)
                                                                                                             aTCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCa                                       >  1:165212/1‑146 (MQ=255)
                                                                                                              tCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAgaga                                   >  2:1130124/1‑149 (MQ=255)
                                                                                                              tCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAACATCGGGCTGCGTGCCCGGTCCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTGTACGGGTAAAAAGCGCGACGCATCAgaga                                   >  2:164310/1‑149 (MQ=255)
                                                                                                              tCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCCGCCCAGCATCGGGCTGCGTGCCTGCTGTCACAAAGCGGTAAACTTAGTGGGTGGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAgaga                                   <  2:273123/149‑1 (MQ=255)
                                                                                                               cGATTAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGaa                                  >  2:198368/1‑149 (MQ=255)
                                                                                                               cGATTAAAAAAATACTGGTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGATTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCaga                                     <  1:674840/146‑1 (MQ=255)
                                                                                                                  ttAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTg                               >  2:838455/1‑149 (MQ=255)
                                                                                                                  ctAACAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTg                               <  2:361760/148‑1 (MQ=255)
                                                                                                                    aaCAAAATACTGTTAAGCAGCGAGTTGGGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAc                             <  2:119302/149‑1 (MQ=255)
                                                                                                                     aCAAAATACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACg                            >  2:523014/1‑149 (MQ=255)
                                                                                                                        aaaTACTGTTTATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACg                            <  1:1005932/146‑1 (MQ=255)
                                                                                                                        aaaTACTGTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACg                            >  1:750826/1‑146 (MQ=255)
                                                                                                                               gTTAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAg                  >  2:392653/1‑149 (MQ=255)
                                                                                                                                ttAATCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGGAAAAAGc                 >  2:12680/1‑149 (MQ=255)
                                                                                                                                   agcggCGAGTTGAGGCCCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGc                 <  1:859937/142‑1 (MQ=255)
                                                                                                                                    tCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACCGGTTGCTTCCTGTGCGCGCAGTATAGCTTTTTCCGTATAAAAAGCGCGAAGCATCAGAGACTTGACGGAGAAAAAAGcc                <  1:737509/146‑1 (MQ=255)
                                                                                                                                    tCAGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCAt             <  2:157574/149‑1 (MQ=255)
                                                                                                                                    tCAGCGAGTGGCGGTCCTGCCTAGCATCTGGCTGCGTGCCTGTTGCCATAAAGTAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGGGAAAAAAGcc                <  1:844908/146‑1 (MQ=255)
                                                                                                                                      aGCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCCTTTTACGGATAAAAAGCGCGCAGCATCAGCCACGTGACAGATAATAAAGCCCATGc           >  2:311790/1‑149 (MQ=255)
                                                                                                                                       gCGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCa          >  2:29368/1‑149 (MQ=255)
                                                                                                                                        cGAGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATGAAGCAGTCTCCTGAACAGGTTGCACCAGTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGCAGCATCAGAGAATTGACGGGGAAAAAAACCCATg            >  1:865221/1‑146 (MQ=255)
                                                                                                                                          cgTTGAGGTTCGGCCCAGCATGGGGCTGCGTGCGTGTTGCCATAAAGCAGTTTCCTTAAAAGGTTGCACCATTTTAGTGCATTCTAGCTTTTTACGGATAAAAAGCGCGAAGCGTCTGAGATTTGACGGAGAAAAAAGCCCATGCa          <  1:1019728/145‑1 (MQ=255)
                                                                                                                                          aGTTGAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCagag       <  2:849591/149‑1 (MQ=255)
                                                                                                                                              gAGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATgg   <  2:573485/149‑1 (MQ=255)
                                                                                                                                               aGGCTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATggg  <  2:1108298/149‑1 (MQ=255)
                                                                                                                                                  aTCTGCCCGGCAGCGGGCTGCGTGCCTTTTTCTATAAACCCGTCTCCTGAATAGGTTGCATCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAACCATGAGAGAATTGAGGGAGAAAAAAGCCCATGCAGAGATggg  <  1:432276/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGG  >  CP009273/4049520‑4049810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: