| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,953,437 | Δ1 bp | 29.4% | pseudogene (178/243 nt) | yifN ← | PemK toxin family pseudogene |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,953,437 | 0 | C | . | 29.4% | 26.9 / 13.9 | 17 | pseudogene (178/243 nt) | yifN | PemK toxin family pseudogene |
| Reads supporting (aligned to +/- strand): ref base C (4/7); new base . (2/3); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATT > CP009273/3953309‑3953572 | aGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTaa < 2:294009/149‑1 (MQ=255) ttAGGGCCTCTATGGGCTACAAAGTCATTGTCGGGGATGGCTGTGTTATTGTCAATTTATGAGCGATATGGCATAGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAGCATTGATTAGAGTACCCTCTGt > 1:61967/1‑149 (MQ=255) gggCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATAAGCAATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTg > 1:173298/1‑149 (MQ=255) ggCTACAAAGTCATTTTCGGGTATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGt < 1:114227/149‑1 (MQ=255) aaGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATCTGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATc < 2:102950/149‑1 (MQ=255) attattGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGGATTGATTGACTTGATGGCCGCCCTCTGTATGTCTGCAATCATCT‑TTGTGTCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTc < 1:140921/149‑1 (MQ=255) aTTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGttt > 2:13930/1‑149 (MQ=255) gTTATTTTAGTGTCAATTTATGAGCCATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACttgtt < 2:169848/149‑1 (MQ=255) tGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTc < 1:13930/149‑1 (MQ=255) atatGGCATTGATTGACTTGATGACCGCCCTCTGTATTTATGCAACCAAC‑AATATAACAAAAAATAATTAAGATACCCTCTGTAAGTCCATGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCtttagaaaaacaaa < 2:124521/149‑12 (MQ=255) ttGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATCAACTATGAAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGaa > 1:59171/1‑149 (MQ=255) gatgaCCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAGCGAATCTGCTGATCAAAGAATTg > 2:47247/1‑149 (MQ=255) gaCCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCa < 2:210488/149‑1 (MQ=255) aCCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAg < 1:269812/149‑1 (MQ=255) aTGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACa < 2:173298/149‑1 (MQ=255) tGTCTGCAACCTACTCATGTGGCAAACATTGTTTAAGAGACCCTTTGTAAGTTCTTGCTGTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACa < 2:42568/149‑1 (MQ=255) tCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACAtt > 1:208711/1‑149 (MQ=255) | AGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATT > CP009273/3953309‑3953572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |