| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,051,961 | C→T | 16.0% | intergenic (‑192/+64) | serA ← / ← rpiA | D‑3‑phosphoglycerate dehydrogenase/ribose 5‑phosphate isomerase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,051,961 | 0 | C | T | 16.0% | 51.6 / 3.4 | 25 | intergenic (‑192/+64) | serA/rpiA | D‑3‑phosphoglycerate dehydrogenase/ribose 5‑phosphate isomerase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (9/12); new base T (2/2); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 | |||||||||||
CCGCCTTTCAACATATCAAAAAATAATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCA > CP009273/3051813‑3052107 | ccGTCTTGCAACATCTCAAAAAATAATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGc < 2:175275/149‑1 (MQ=255) gCCTTTCAACATATCAAAAAATAATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGt < 2:100197/149‑1 (MQ=255) tatCAAAAAATAATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAAGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCa > 1:176118/1‑149 (MQ=255) atCAAAAAATAATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGTGTGACACATGTCAc > 1:43552/1‑149 (MQ=255) atCAAAAAATAATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGTGTGACACATGTCAc > 1:43564/1‑149 (MQ=255) tatGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATAAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGCAGAGAGttttt > 1:25133/1‑149 (MQ=255) tatGAACGGTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAAttttt < 2:294721/149‑1 (MQ=255) aTGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTcccc > 1:92335/1‑149 (MQ=255) ggAAATATCACGCAAATGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACACGCCACCAAATTTAATGAAGAGAATTTTTTTAACGGCGGAGGTTCCCCCGTCAGa < 2:324272/149‑1 (MQ=255) ggAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGa > 2:17408/1‑149 (MQ=255) aaTATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCa < 2:54959/149‑1 (MQ=255) tCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGAATTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTc > 1:295057/1‑149 (MQ=255) aaaGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTGATTTAGAGCAATCCCCAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATg > 2:287156/1‑149 (MQ=255) gTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGttt < 2:281172/149‑1 (MQ=255) tAATTCTTGGATGAATATGCTGCTTTAGAGGATTTATTTAGAGCAATCGACAATTGCTTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTGCCCCCGTCAGATTATTTCACAATGGtttt < 1:205869/149‑1 (MQ=255) aTGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGACGAGAATTTTTTTAACGGGGGCGGGTCCCCCGTCAGCTCATTTCACAAGGGTTTTCACACCGTCa > 2:71382/1‑149 (MQ=255) aaTATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGTGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGgt < 2:43552/149‑1 (MQ=255) aaTATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGTGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGgt < 2:43564/149‑1 (MQ=255) ttGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGtgtg < 1:195737/148‑1 (MQ=255) gATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATGAGCGCAAc > 2:249703/1‑149 (MQ=255) tttaGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCcgc > 2:259060/1‑149 (MQ=255) gCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACg < 1:300333/149‑1 (MQ=255) gCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACg < 1:300217/149‑1 (MQ=255) aaTCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTTACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGt < 1:107458/149‑1 (MQ=255) tccataactGCCTGGTAAATGCCTTACACGTGTCACCAAATGTAATGCAGAGCATTTTTTTGACGGGTGAGGTTCCGCCGCCAGTTCTTCTCGTCATGGTCTTGACCCCTTCAGGTGTGCCGATCAGCGCAACGTCCGCGCCACGGTTa < 2:162660/141‑1 (MQ=255) tAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACa < 1:301594/149‑1 (MQ=255) atcGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGTAGGTTCCCCCGTCAGACAATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGTTTAGCAAACAAGCCAACAGTca < 2:118835/147‑1 (MQ=255) | CCGCCTTTCAACATATCAAAAAATAATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCA > CP009273/3051813‑3052107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |