Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,471,768 G→A 14.5% A30V (GCG→GTG)  slyD ← FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,471,7680GA14.5% 53.3 / 3.7 29A30V (GCG→GTG) slyDFKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base G (12/12);  new base A (2/2);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

CACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGT  >  CP009273/3471622‑3471915
                                                                                                                                                  |                                                                                                                                                   
cccGTTGCCACAGCTTTTCTTCGTACTGACTGTACGCGTCGTTCGTGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCCGGGCACCGTGACCATGCAGGTAGTCCAGCGGCCCa                                                                                                                                                   <  2:309252/147‑1 (MQ=255)
        aCCAGGTTTTCGTCATACTGACCGTAAGCGACCTTCGCACGAACAGCGAGATCAAATTTGTCGCCAACTTCATGAGCTTCCAGCGCCGTTTCCTGGCCAGACATCAGGGAACCGTGACCATGCAGGGAGTCCAGCGGCGCACACACCgg                                                                                                                                           >  1:245069/1‑149 (MQ=255)
                       tACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCCCCGGAGACTCATcaaccaa                                                                                                                            >  1:46612/1‑149 (MQ=255)
                            aCCGTAAGCGTCGTTCGCCCCAACCGCCACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCTGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAacacac                                                                                                                       <  1:100123/149‑1 (MQ=255)
                                    cgtcgtTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtcttct                                                                                                               <  1:301857/149‑1 (MQ=255)
                                    cgtcgtTCGCGCCAACAGCGACACAAAATTTAACGAAAAAATCATGAACTTGAGTCGTCGCTAGTAGGCCAGAGATCAGGGACCCGTGACCAGTCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtcttct                                                                                                               <  2:323355/149‑1 (MQ=255)
                                        gtTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgtac                                                                                                           <  2:43589/149‑1 (MQ=255)
                                                  aCAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCCCCGGAGACTCATCAACCAACACACCCTCTTCTgcacataaaaaata                                                                                                 >  2:106988/1‑142 (MQ=255)
                                                     gCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCACACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGc                                                                                              >  2:175641/1‑149 (MQ=255)
                                                     gCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCACACTCACCGGAGACTCATCAACCAACACACCGTCGTCTATACGTACCTGCTAGGc                                                                                              >  2:175689/1‑149 (MQ=255)
                                                             aaaTTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGa                                                                                      <  1:171205/149‑1 (MQ=255)
                                                                      gCCAACTTCATGACCTCCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGt                                                                             <  2:33425/149‑1 (MQ=255)
                                                                        cAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGAGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCAACAGGCCt                                                                           >  1:160365/1‑149 (MQ=255)
                                                                           cgtcctGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCAGACACTCAACAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCtttt                                                                        <  2:286798/144‑1 (MQ=255)
                                                                            ttCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAAGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTg                                                                       >  1:210021/1‑149 (MQ=255)
                                                                                  aCCTTCCAGCGCCGTTTCCAGGCCAGAGATCATGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGTCTCATCAACCAACACATCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACtt                                                                 <  1:159808/149‑1 (MQ=255)
                                                                                  aCCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGGCTTCTGTACGCGCCTGATAGGACCGGCTGACCACCAGGCCTTTTGCAACtt                                                                 >  2:209124/1‑149 (MQ=255)
                                                                                      tCCAGCGCCGTTTCCAGGCCAGAGGTCAGGGAACCGTGACCACGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGATGTCCACCAGGTCTTTTGCTACTTTCAt                                                             <  2:251975/149‑1 (MQ=255)
                                                                                      tCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCAt                                                             <  1:205386/149‑1 (MQ=255)
                                                                                         aGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGGCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGAACCTGACAGGACCGGCTAGCCACCAGGTCATTTGCTTCATTCATgtc                                                          >  2:280778/1‑147 (MQ=255)
                                                                                           cgcCGTTTCCAGGCCAGAGCTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACACACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGatat                                                        >  1:43817/1‑149 (MQ=255)
                                                                                                tttCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTTTTTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCt                                                   <  1:12926/149‑1 (MQ=255)
                                                                                                  tCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTAACCGGAGACTCATCAAACAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGa                                                 >  1:299537/1‑149 (MQ=255)
                                                                                                        ccAAAGAACAGTGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCCTCAACCAACACACCGTCTCCTGTACGTACCTGCTAGCGCAGGCTGACCCCGCGGTCTTTTGCCGCTTTCAGGCTAGCTGCTGacaactg                                           >  2:98345/1‑143 (MQ=255)
                                                                                                           gagaTCAGGGAACCGTGACCATGCAGGTATTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGgaa                                        <  2:245069/149‑1 (MQ=255)
                                                                                                                       ccGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACAAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGGAGAATAGTGgcgc                            >  1:184754/1‑149 (MQ=255)
                                                                                                                              cATGCAGGTAGTCCAGCGGCACACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGt                     <  1:175689/149‑1 (MQ=255)
                                                                                                                              cATGCAGGTAGTCCAGCGGCACACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGt                     <  1:175641/149‑1 (MQ=255)
                                                                                                                               aTGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTa                    >  1:329437/1‑149 (MQ=255)
                                                                                                                                                 cgcTCTCACCGGAGTCTCATCACCCAACACCCCGGCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCCGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGTAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCgt  <  2:295386/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGT  >  CP009273/3471622‑3471915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑