Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,770,381 G→A 15.1% A119A (GCG→GCA)  yibL → ribosome‑associated DUF2810 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,770,3810GA15.1% 48.2 / 3.6 28A119A (GCG→GCA) yibLribosome‑associated DUF2810 family protein
Reads supporting (aligned to +/- strand):  ref base G (16/6);  new base A (2/2);  total (18/10)
Fisher's exact test for biased strand distribution p-value = 5.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

GCCATTCCAGCGCGCAATTACCAAAAAAGAGCAGGCTGATATGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAC  >  CP009273/3770234‑3770523
                                                                                                                                                   |                                                                                                                                              
gCCATTCCAGCGCGCAATTACCAAAAAAGAGCAGGCTGATATGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGAGTGGGTTTTCAAAGACCGCGt                                                                                                                                               >  2:222490/1‑149 (MQ=255)
gCCATTCCAGCGCGCAATTACCAAAAAAGAGCAGGCTGATATGGGCAAGCTGAAGAAAAATGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGCAATGGGCCTGCAGGAGATGACTGGGTTGTCAAAGACAGCGt                                                                                                                                               >  1:194299/1‑149 (MQ=255)
   aTTCCAGCGCGCAATTACCAAAAAAGAGCAGGCTGATATGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGGTTTCAAAGACCGCGgttt                                                                                                                                            >  2:271139/1‑149 (MQ=255)
    ttCCAGCGCGCAATTACCAAAAAAGAGCAGGCTGATATGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTa                                                                                                                                           >  1:284820/1‑149 (MQ=255)
    ttCCAGCGCGCAATTACCAAAAAAGAGCAGGCTGATATGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACAGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTa                                                                                                                                           >  2:237519/1‑149 (MQ=255)
            cgcAATTACCAAAAAAGAGCAGGCTGATATGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAAcaca                                                                                                                                   >  1:213434/1‑149 (MQ=255)
                                  gCTGATATGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGca                                                                                                             >  2:35380/1‑149 (MQ=255)
                                       tatGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTc                                                                                                        >  2:97898/1‑149 (MQ=255)
                                           ggCAAGCTGAAGAGAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCTTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCtat                                                                                                    <  1:191362/149‑1 (MQ=255)
                                                         aaGTGTTCGCGGACTGGTCGTTGTGCCCCCAATGACCGCACTGGGCCACAAAATGAGCCTGCAGGAGATAACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAggg                                                                                      >  1:17646/1‑148 (MQ=255)
                                                                      cTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTTATTCTGTACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCAt                                                                         <  1:94007/149‑1 (MQ=255)
                                                                       tGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCATTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATc                                                                        >  1:121991/1‑149 (MQ=255)
                                                                       tGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGAGCCTGCAGAAGATGACTGGGTTTTCAAAAACCGCATTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATc                                                                        >  1:121860/1‑149 (MQ=255)
                                                                         gTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCg                                                                      >  2:319778/1‑149 (MQ=255)
                                                                         gTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCg                                                                      >  2:71996/1‑149 (MQ=255)
                                                                                tgCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCTGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGAt                                                               <  1:234480/149‑1 (MQ=255)
                                                                                       aaTGACCGCACTGGGCCGCGAAATGGGCCTGGCTTGCATGTCTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGgcgc                                                        >  1:76694/1‑149 (MQ=255)
                                                                                             cGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACGGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGGGTTCACGCCGCATCCGGAAAGATAAGGCGCGCTggg                                                  >  2:169734/1‑148 (MQ=255)
                                                                                                    gacggcgAAATGGTCCTGCAGGAGAGGACTGGGTTTTCAAAGCCCGCGTTTTAAGAACACAGTATCTACAGTGTGATTCTGTACATGCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATcaacaa                                           <  2:194299/145‑1 (MQ=255)
                                                                                                           aaaTGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCTGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAgg                                    <  1:246318/149‑1 (MQ=255)
                                                                                                           aaaTGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCTAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAgg                                    <  1:97898/149‑1 (MQ=255)
                                                                                                                              gACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGGTGAGGAt                 >  2:337441/1‑149 (MQ=255)
                                                                                                                                  ggtttttCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTGTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGcc             <  1:237519/146‑1 (MQ=255)
                                                                                                                                   ggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCGATTCTATGCTGAGGCTTGcca            >  2:151893/1‑148 (MQ=255)
                                                                                                                                       ttCAAAGACCGCTTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCttct        <  2:3235/149‑3 (MQ=255)
                                                                                                                                         cAAAGACCGAGTTTTAAGAACACAGTATCTACAGAGAAATTCCGCACATTCCTATAGGCCGAGTAAGGAGATCACGCCACATCCGGCAAAATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGTTAAGGATTGCCCCTTTCt      >  2:93698/1‑149 (MQ=255)
                                                                                                                                             gACCGCATTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAc  <  2:121860/149‑1 (MQ=255)
                                                                                                                                             gACCGCATTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAc  <  2:121991/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GCCATTCCAGCGCGCAATTACCAAAAAAGAGCAGGCTGATATGGGCAAGCTGAAGAAAAGTGTTCGCGGACTGGTCGTTGTGCACCCAATGACCGCACTGGGCCGCGAAATGGGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAC  >  CP009273/3770234‑3770523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑