| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 717,981 | C→T | 18.6% | L630L (CTG→CTA) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 717,981 | 0 | C | T | 18.6% | 31.3 / 2.9 | 21 | L630L (CTG→CTA) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
| Reads supporting (aligned to +/- strand): ref base C (8/9); new base T (2/2); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01 | |||||||||||
AATCATGCGAAAGCGCCGCCAGCAGGGCGTTGCGGATCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAAAATCTGGTACGGTACACCGGGTAACGTGTCGGTGCCCGCGCCCGCAGGCAGGC > CP009273/717848‑718126 | aaTCATGCGAAAGCGCCGCCAGCAGGGCGTTGCGGATCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTcc > 1:190815/1‑149 (MQ=255) tCATGCGAAAGCGCCGCCAGCAGGGCGTTGCGGATCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCgg > 2:99121/1‑149 (MQ=255) agtcGAAAGGGCCGCCAGCAGGGCGTTGCGGATCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGTGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCGGTTCCGGGa < 2:113580/146‑1 (MQ=255) ccgccAGCAGGGCGTTGCGGATCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTgg > 1:135111/1‑149 (MQ=255) ccAGCAGGGCGTTGCGGATCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGTCTAACAGTGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGgcgc < 1:88125/149‑1 (MQ=255) cGGAGCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCGGTTCCGGGATCATCAACTGGCGCAGGTGCCCCGGCTc > 2:211430/1‑149 (MQ=255) ggATCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCGACTGGCGCAGCGTCCCCGGCTcc > 2:24435/1‑149 (MQ=255) aCGGTCGATTGCCATCCGCGCCGGTTTTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTTCGGGATCATCAACTGGCGCAGATTCCCCGGCTccaccaccacca < 1:109622/149‑1 (MQ=255) gTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGc < 1:120566/149‑1 (MQ=255) cgcgCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTtctc < 2:135111/149‑1 (MQ=255) gTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCCAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCtt > 1:245380/1‑149 (MQ=255) ttcGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGTAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTa > 1:224333/1‑149 (MQ=255) ttcGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGTAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTa > 1:224244/1‑149 (MQ=255) cTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAAAATCTGGTACGGTacac < 2:190815/149‑1 (MQ=255) aaGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAACATCTGGTACGGTACACCgg > 1:238632/1‑149 (MQ=255) aGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCGCCAGCCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAAAATCTGGTACGGTACACCggg < 1:28665/149‑1 (MQ=255) cGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAAAATCTGGTACGGTACACCGGGTAACGTGTCg < 1:99121/149‑1 (MQ=255) cTAACAGCGTAAACGTCTCCAGTAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGTCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAAAATCTGGTACGGTACACCGGGTAACGTGTCGGTg < 1:320062/149‑1 (MQ=255) cTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAAAATCTGGTACGGTACACCGGGTAACGTGTCGGTg > 1:234260/1‑149 (MQ=255) tAACAGAGGAAACATCTTCCGTCGGCGCCGCTGTCCCGGGGTCACCACCTCGCGGAGGTCCCTCGTGCCCACCACCACCGGCCCGGTGGTTTCCTCGCCGCTTTTTAGCGGCAAAAGCTGGTACGGTACGCCGGGTAACGTGTCGGTgc < 2:127248/149‑1 (MQ=255) tcCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAAAATCTGGTACGGTACACCGGGTAACGTGTCGGTGCCCGCGCCCgcaggcag < 1:211430/149‑1 (MQ=255) ccgcagGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGTCGCTTTTTAGCGGCAAAATCTGGTACGGTACACCGGGTAACGTGTCGGTGCCCGCGCCCGCaggcaggc < 1:24435/147‑1 (MQ=255) | AATCATGCGAAAGCGCCGCCAGCAGGGCGTTGCGGATCTGTTCACGTTCGCTTGCCATCCGCGCCTGTTCTTCGCTGGCGGTTAGCGTCAGCCGCTCAAGGGCATTGGCGACTAACAGCGTAAACGTCTCCAGCAGGCGCTGCTGTTCCGGGATCATCAACTGGCGCAGATTCCCCGGCTCCACCACCACCAGCCCGTAGGTTTTCTCGCCGCTTTTTAGCGGCAAAATCTGGTACGGTACACCGGGTAACGTGTCGGTGCCCGCGCCCGCAGGCAGGC > CP009273/717848‑718126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |