| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,960,101 | C→A | 13.4% | R109S (CGC→AGC) | rho → | transcription termination factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,960,101 | 0 | C | A | 13.4% | 77.6 / 3.1 | 35 | R109S (CGC→AGC) | rho | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base C (18/11); new base A (3/2); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTT > CP009273/3959965‑3960246 | tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGATTCCCCTAGCCAAATCCGCAGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCACCGAAAGAAGGTGAACGCTATTTTgcgc > 1:121115/1‑149 (MQ=255) ctccGTTCCGCAGACAGCTCCTCCCTCGCCGGTCCTGAAGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGACAGACGGGGAACGCTATGTTGCgctgct > 1:81506/1‑149 (MQ=255) ctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTACAAACATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCGGGTGAGAATCGCCCGCCTAAAGAAGAGGAACGCTATATTGCgctgct > 1:104160/1‑149 (MQ=255) ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCGATTTTGCGCTGCTGaaaa > 2:282099/1‑148 (MQ=255) ccGCAGCCGCCTCCTACCCCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCCCCGCACCGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt < 2:21059/149‑1 (MQ=255) ccgCTCCTACCTCGCCGGTCCTGAGGACATCTACCTTTCCTCTACCCAATGCCGCCGTTTCAACCCCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg < 2:184631/147‑1 (MQ=255) catcTCCTGCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAACTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGGTGACGAAg < 2:201564/146‑1 (MQ=255) cAGCTCCTACCTCGCCGGTCCTTATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTCGTAAGATTCGCCCGCCGAAAGAAGGTGAAAGCTATTTTGCGCTGCTGAAAGTTAACGAAg < 2:66579/149‑1 (MQ=255) cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCTCGCCGAAAGAAGGTGAAAGCTATTTTGCGCTGCTGAAAGTTAACGAAg < 1:228866/149‑1 (MQ=255) cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGGAGGTGAACGCTATTTTGCGCTGCGGACAGTTAACGAAGTTAACTTCGaga > 2:209527/1‑147 (MQ=255) ccGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAATGTTTTTTTGCGCTGCTGAAAGTGAACGAAGTTAACTTCGACaaa < 1:19651/149‑1 (MQ=255) gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACCAACCt > 1:266266/1‑149 (MQ=255) cTGATGACATCTACGTTTCCCCTGGCCAACTCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaa < 2:168722/149‑1 (MQ=255) ttCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa < 1:189681/149‑1 (MQ=255) aGCCAAATTCGCCGTGTCAAACTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAAAGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt > 2:252230/1‑149 (MQ=255) ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGATAACGAAGTTAACTCCGACAAACCAGAAACCGCCAGCAACAAAAACACCTTTg > 2:291793/1‑149 (MQ=255) aaaTCCGCCGTTTCAACCTCCGCCCTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGGAAACGCCCGCAACAAAGTCCTCTTTgag > 1:277682/1‑149 (MQ=255) tCCGCCGTTTCACCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGATGTTAATTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc < 1:19381/149‑1 (MQ=255) tCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTTTCGTTGTTTATTTTGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc < 1:19544/149‑1 (MQ=255) ccgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGATATTTTGCTCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAAGAAAATCCTCTTTGAGAACt > 1:41231/1‑149 (MQ=255) gccgTTTCAACCTCCGCACTGGTGATACCATCTCTCGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTTACAGTTAACGAAGTTAACTTCGACAAACCTGAAGACGCCCGAAACCAAATCCACTTTGAGAACTTa > 2:202865/1‑149 (MQ=255) tttCAACCTCCGCACTGGTGATACCATCACTGGTAAGATTGGCCCGCCGAAGGAAGGAAGACGCTATTTTGCGTTGATGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCACCAAAATCCTCTTTGAGAACTTAAccc > 2:24684/1‑149 (MQ=255) tCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg < 2:278019/149‑1 (MQ=255) aaCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAAAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGATAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCCCt > 1:67225/1‑149 (MQ=255) cTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAAGTGAAAGAAATTTTACGATGCTGAAAGTTAAAGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATACTCTTTGAGAACTTAACCCCGCGGCACGCAAAc > 1:2600/1‑149 (MQ=255) ggTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActc > 1:231233/1‑149 (MQ=255) ggTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGAACGCAAActc > 1:231231/1‑149 (MQ=255) aaGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGCGAACTTACCCCCGCTGCACGCAAACTCTCGTCTGCGtgcgcaacg > 2:273145/1‑141 (MQ=255) aGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGAACGCAAACTCTCGTCTGCGTATGGAACGt > 2:75754/1‑149 (MQ=255) cccgccGAAAGAAGGTGAAATCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCCAACTCTCGTCTGCGCATGGCACGTggccacg > 2:297797/1‑144 (MQ=255) ccgccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg > 1:97973/1‑149 (MQ=255) gAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTa < 2:121115/149‑1 (MQ=255) gAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTGAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGCATGGAACGGGGTAACGGtttgc > 2:73853/1‑146 (MQ=255) agGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGTTGTACGCTATCTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAg < 1:261030/149‑1 (MQ=255) gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCTTATTTTCGTCTGCGTATGGAACGTGGGAACGGTTCTACTGAAGAttt < 1:5011/149‑1 (MQ=255) | TTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTT > CP009273/3959965‑3960246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |