| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,933,356 | G→A | 13.3% | intergenic (+8/+123) | pykA → / ← lpxM | pyruvate kinase II/myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,933,356 | 0 | G | A | 13.3% | 69.3 / 3.0 | 31 | intergenic (+8/+123) | pykA/lpxM | pyruvate kinase II/myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (16/10); new base A (2/2); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
GCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAA > CP009273/1933211‑1933495 | ccaccTGCCAGATAAGTTGTTAATCTGCTGCGCTATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGTCGACGTGATTAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCg < 2:211920/145‑1 (MQ=255) cAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATgcggc > 2:599/1‑149 (MQ=255) cGGTTAATCTGCTGCGAGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTCACGGTAGAGTAATGACGTTGCCGGATGCGGCGaaaagcg > 2:73688/1‑146 (MQ=255) tggtgaTCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGcc < 2:338456/144‑1 (MQ=255) gTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCca > 1:159947/1‑149 (MQ=255) gTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCATGGGTTCTACTAATACCACGCGTATTTTAACGGGAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCca < 2:2039/149‑1 (MQ=255) gctgcGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGc < 1:206123/149‑1 (MQ=255) ctgcGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGcc > 1:200359/1‑149 (MQ=255) gATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTACCGGATGCGGCGAAAACGCCACATCCGGCCTACAGt > 1:87717/1‑149 (MQ=255) gATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGAGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTACCGGATGCGGCGAAAACGCCACATCCGGCCTACAGt > 1:87705/1‑149 (MQ=255) ttACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGCGCAAGTACGTTGCCGGATGGGGCGAAAACGCCGCATCCGGCCTACGCTTGAATggt > 2:169970/1‑147 (MQ=255) tACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGTGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATa < 1:599/149‑1 (MQ=255) tACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTTTCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATa < 1:42373/149‑1 (MQ=255) aTGTCTGGTGCCCTGGGTATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCaa < 2:202875/149‑1 (MQ=255) tGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGATTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGAt > 1:202855/1‑149 (MQ=255) cTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCCAGAAAGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAat > 2:322386/1‑149 (MQ=255) tGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGaa > 1:203070/1‑149 (MQ=255) aCCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGTGATTTACTGGCCATATTCGTCCCACAGTTCAATGATAGTTAAACAGATTTCGAATATTCTGAAGCaa < 2:323838/149‑1 (MQ=255) aCCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCaa > 1:181484/1‑149 (MQ=255) aCCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTAAACAGATTTCGAATATTCTGAAGCaa > 2:11612/1‑149 (MQ=255) cccAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAAAGGTACGGTCAGAACATGGCCGGATGCAGCGACAGCGACTCCCCCGTTGCGCTGGTCGATGATAGTTCAACAGATTTCGAATATTTTGGAGCaga > 2:65959/1‑147 (MQ=255) ccAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAAc > 1:228289/1‑149 (MQ=255) aGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAg > 2:262159/1‑149 (MQ=255) tACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGc > 2:103414/1‑149 (MQ=255) ccACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCCTCGCCTCGCgaga < 2:200359/149‑1 (MQ=255) aCGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCATCTCCTCGCGAGAgg > 2:74195/1‑149 (MQ=255) tAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTAtt < 2:311444/149‑1 (MQ=255) ggTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGAGAGAGGCTTTTTTATTTAAt > 1:157449/1‑149 (MQ=255) ggTAGAGTAAGTACGTTACCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGAt < 2:87705/149‑1 (MQ=255) ggTAGAGTAAGTACGTTACCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGAt < 2:87717/149‑1 (MQ=255) aagtaCGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATaaa < 2:203070/149‑1 (MQ=255) | GCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAAGTACGTTGCCGGATGCGGCGAAAACGCCACATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATATTCTGAAGCAAACTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAA > CP009273/1933211‑1933495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |