Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,219,065	G→A	13.9%	Q16* (CAG→TAG)	yohC ←	Yip1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,219,065	0	G	A	13.9%	56.0 / 2.1	27	Q16* (CAG→TAG)	yohC	Yip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (10/13);  new base A (2/2);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

CAGGATAGTGCCATCGCCAAAATTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTC  >  CP009273/2218928‑2219202                                                                                                                                          |                                                                                                                                          cAGGATAGTGCCATCGCCAAAATTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCTTCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGAt                                                                                                                                <  1:187862/149‑1 (MQ=255)       aGTGCCATCGCCAAAATTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAATTTGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGAt                                                                                                                          <  1:324086/149‑1 (MQ=255)        gTGCCATCGCCAAAATTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAATTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATg                                                                                                                         <  1:309682/149‑1 (MQ=255)                 ccAAAATTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTACATTTCACGATCGGGATGGGAAAACAg                                                                                                                >  2:3989/1‑149 (MQ=255)                 ccAAAATTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTACATTTCACGATCGGGATGGGAAAACAg                                                                                                                >  2:3985/1‑149 (MQ=255)                   aaaaTTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTc                                                                                                              >  2:233879/1‑149 (MQ=255)                   aaaaTTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTc                                                                                                              >  2:167044/1‑149 (MQ=255)                                             cAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGCGTGTAATGGTTAGAAATCGGTTCGGTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGCAAACAAGTCCCCAGCCATGGCTCATACCGCCCCCt                                                                                    >  2:112952/1‑149 (MQ=255)                                              aaTGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCtt                                                                                   >  2:51516/1‑149 (MQ=255)                                               aTGAGGGCGCAAATCACCGGAAGCGCCGCCAGCACAAAACCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCTATTAATCCCCTGCATTTCACCATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCttg                                                                                  <  2:151590/149‑1 (MQ=255)                                                tGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCttgt                                                                                 <  1:161070/149‑1 (MQ=255)                                                tGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATAGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCCTTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTGATACAGGCCCCtcgt                                                                                 >  2:87239/1‑149 (MQ=255)                                                 gAAGGCCCAAACCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCttgtt                                                                                <  2:10232/149‑1 (MQ=255)                                                             cACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGCGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGa                                                                    <  2:147434/149‑1 (MQ=255)                                                                   aaacgccgcCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGa                                                              <  2:230309/146‑1 (MQ=255)                                                                     tCGCCGCCATCAGCCAAACGTGGTGTGTGTCATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCCCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACa                                                            <  2:251177/149‑1 (MQ=255)                                                                        ccgccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTACATTTCACGATCGGGATGGGAAAACAGTCCCTTGACATGGTTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGtt                                                         <  1:3989/149‑1 (MQ=255)                                                                        ccgccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTACATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGTTTATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGtt                                                         <  1:3985/149‑1 (MQ=255)                                                                                      aCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAg                                           >  1:283424/1‑149 (MQ=255)                                                                                            tgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTACGCTtgtg                                     >  1:26768/1‑149 (MQ=255)                                                                                             gggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCAATTCACGATCGGGCTGGGAAAACAGTCCCCAGACATGGCTCATACTGCCCCCTTGTTGTGAGTTCATGAAGCTGCCAATTCAAGTATAAGTAAGGTagtgg                                    >  2:57876/1‑148 (MQ=255)                                                                                                            aaaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGc                     >  1:247203/1‑149 (MQ=255)                                                                                                             aaTCGTTTGGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGATTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGcc                    <  2:280234/149‑1 (MQ=255)                                                                                                                          tCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCa       <  1:316493/149‑1 (MQ=255)                                                                                                                          ccgcgATTAACCACCTGCATTTCACGATCGGGATGGGAAAACAGCCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCa       <  1:87239/148‑1 (MQ=255)                                                                                                                               aTTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGACCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTc  >  1:74091/1‑149 (MQ=255)                                                                                                                               aTTAATCACCTGCATTTCACGAGCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTACAACTCAACTTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTc  <  2:215552/149‑1 (MQ=255)                                                                                                                                          |                                                                                                                                          CAGGATAGTGCCATCGCCAAAATTCCAGCCAATCTGTGTAGTGCCAATGAAGGCGCAAATCACCGGAATCGCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTC  >  CP009273/2218928‑2219202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑