| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,249,251 | A→G | 19.1% | N140D (AAT→GAT) | yeiL → | putative transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,249,251 | 0 | A | G | 19.1% | 27.6 / 4.6 | 21 | N140D (AAT→GAT) | yeiL | putative transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (7/10); new base G (2/2); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01 | |||||||||||
CCTTGCGCTCCCTATGAAACATTACCGTCCGCTGTTATTAAACGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAA > CP009273/2249112‑2249398 | ccTTGCGCTCCCTATGAAACATTACCGTCCGCTGTTATTAAACGACACACTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGGATCGCCTgg > 1:206748/1‑149 (MQ=255)ccTTGCGCTCCCTATGAAACATTACCGTCCGCTGTTATTAAACAACACGCTATATTTAAGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGGATCGCCTgg > 1:206813/1‑149 (MQ=255) ctcTCCCTCTGCAACATTACCGTCCGCTGTTATTAATCAACACGGTTTGTGAACGGAGTATGGGCATCAACTGGATTCATAAGCATAACCATAATAGTGGTAATTAAACTCTGAATCAATTATTTCCGTTAGAGAATCGCCTGgcggca < 2:158789/147‑1 (MQ=255) tCCCTATGAAACATTACCGTCCGCTGTTATTAAACGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCAttt < 2:61083/149‑1 (MQ=255) ccTATGAAACATTACCGTCCGCTGTTATTAAACGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATAATTTCCGTTAGTGAATCGCCTGGCAGCATTtat > 1:204499/1‑149 (MQ=255) tACCGTCCGCTGTTATTAAACGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCa < 2:311647/149‑1 (MQ=255) attaAACGGCACGCTATTTTGTCGAACACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCttt < 2:220763/149‑1 (MQ=255) ttaAACGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTa > 1:206345/1‑149 (MQ=255) aaCGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCa > 1:78102/1‑149 (MQ=255) ttttACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTACTATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAATGTGATATTTATCACGgaaacaaaacg > 2:261676/1‑142 (MQ=255) tACGAAAACTCTGCGTCACCTTAAGTCATAAATATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCaa > 1:163601/1‑149 (MQ=255) cctctGCGTCACCTTAAATCATAAAAATTATCTTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCTCTTGGCATCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCag < 1:152058/148‑1 (MQ=255) ctctGCGTCACCTTACGTCATAAAAATTATCGTAATATTGTTTCTTTTACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGTATTTATATTACTCTCGCAGGAAGGTGAGCTTTATCACGAAAAGCATACGCAAGCGGCaga < 1:28981/149‑1 (MQ=255) ctctGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCaga < 1:141659/149‑1 (MQ=255) gCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTACGTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGGGATCTTTATCACGAGAAGCATCCGCAAGCGCCAGATTAt > 2:29028/1‑149 (MQ=255) tCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATAATTTCCGGCAGAAAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCtt > 1:317349/1‑149 (MQ=255) aaaTTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTTATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGAc < 1:108421/149‑1 (MQ=255) aTCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGGATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCtt < 2:206813/149‑1 (MQ=255) aTCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGGATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCtt < 2:206748/149‑1 (MQ=255) tcggtttatTGTTTCTTCAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTTCTCTCGCAGTAAGGTGATCTTTATCACGAAAAGTATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCttt < 1:136015/143‑1 (MQ=255) gAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTaaaa < 2:274650/149‑1 (MQ=255) | CCTTGCGCTCCCTATGAAACATTACCGTCCGCTGTTATTAAACGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAA > CP009273/2249112‑2249398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |