| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,329 | C→A | 73.2% | intergenic (‑62/+159) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,329 | 0 | C | A | 73.2% | 24.7 / 10.8 | 24 | intergenic (‑62/+159) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base A (10/7); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01 | |||||||||||
CGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCA > CP009273/2420193‑2420465 | cGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGAc < 2:271188/149‑1 (MQ=255) caAACGCCGCAGTTGCGCTGGAGCAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaag > 1:106457‑M1/1‑131 (MQ=255) cgccccATTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGCTAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGATTATTTTCATGTTTGTTATTTTTATCGTGCCATAAAAGTGACCATCAAGGCAAAata < 1:11445/149‑1 (MQ=255) aggtgctCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatc < 2:293143‑M1/146‑29 (MQ=255) ttGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccctctaggcaaaatccccc > 2:202456‑M1/1‑119 (MQ=255) ttGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTGTTTTCATAATGTCCTCAACTGACAGTCTTCTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaatatatcca > 2:324313‑M1/1‑119 (MQ=255) gCCAGAACCAGAGGGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGCCACATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtatta > 1:224494/1‑149 (MQ=255) aCCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgctagacgtattatt > 2:313390‑M1/1‑99 (MQ=255) agggagagggaGAGCACCAGTTTTTTCATAGTTTTCTCCACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttatacgtattattttt < 2:145550‑M1/146‑54 (MQ=255) agCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAAGCAAAATATCCCGCCCCGTTAGACGGTTTATTTTTGATTcaaaaa > 2:79184/1‑148 (MQ=255) cGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaa < 1:242759‑M1/149‑62 (MQ=255) aCCAGTTTTTTTATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattctttttcattaaaaatgctta > 2:138416‑M1/1‑81 (MQ=255) ttttCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgc > 2:260722‑M1/1‑74 (MQ=255) tttAATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcacggccaaatatcctgccccgttagacgtattatttttcattaaaaatgcttaagtatgca < 2:50120‑M1/149‑77 (MQ=255) aaTGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttacttatgcgattcaa > 2:27677‑M1/1‑67 (MQ=255) tCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGTCTGCTagtgaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcacca < 1:332023‑M1/149‑90 (MQ=255) cTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgat > 1:204984‑M1/1‑56 (MQ=255) ttttATAGTGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCt < 1:301179/149‑1 (MQ=255) ttttATAGGGAATATTTACTGGTTTGTTACACTTATCGTGCCATAAAATTGTCCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCt < 1:275528/149‑1 (MQ=255) ttATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaagacaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaacacgatagtgcaacaaac > 2:95701‑M1/1‑44 (MQ=255) ggAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCTCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa > 2:96591/1‑149 (MQ=255) tttACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcacc < 2:106457‑M1/149‑118 (MQ=255) ggTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggccaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtattcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatg < 1:97615‑M1/149‑124 (MQ=255) acacTTATCGTAACATAAaattaattgaccatcaaggcaatagaccccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcattcc < 1:313390‑M1/149‑132 (MQ=255) | CGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCA > CP009273/2420193‑2420465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |