Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 572,216 C→T 30.8% pseudogene (66/1040 nt) nmpC ← DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273572,2160CT30.8% 10.5 / 6.1 13pseudogene (66/1040 nt)nmpCDLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
Reads supporting (aligned to +/- strand):  ref base C (4/5);  new base T (2/2);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

CGTTGATTTGGGTTTCACCTTTGAAGCCAAGACGGGCATAAGTAGTATCACCATCATCTGCATCATTAGAGGAGAAGTAGTGCTTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATCAATGTG  >  CP009273/572071‑572353
                                                                                                                                                 |                                                                                                                                         
cGTTGATTTGGGTTTCACCTTTGAAGCCAAGACGGGCATAAGTAGTATCACCATCATCTGCATCATTAGAGGAGAAGTAGTGCTTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGcc                                                                                                                                           >  2:191431/1‑146 (MQ=255)
                 ccTTTGAAGCCAAGACGGGCATAAGTAGTATCACCATCATCTGCATCATTAGAGGAGAAGTAGGGATTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTAGCTTTATTATCAATTTCAGCTGCCTGCGCCGAAATCGCCAt                                                                                                                          >  2:45408/1‑146 (MQ=255)
                     tGAAGCCAAGACGGGCATAAGTAGTATCACCATCATCTGCATCATTAGAGGAGAAGTAGTGCTTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGt                                                                                                                      <  2:212304/146‑1 (MQ=255)
                                                   catcatCTGCATCATTAGAGGAGAAGTAGTGCTTAGCATTAACTTTGCCGTACAGATCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCCCGGTTa                                                                                    >  1:56410/1‑150 (MQ=255)
                                                               catTAGAGGAGAAGTAGTGCTTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGCTTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCg                                                                        >  1:207426/1‑150 (MQ=255)
                                                               catTAGAGGAGAAGTAGTGCTTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGCTTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCAACTGTTAATTTTTTCATCg                                                                        >  1:207320/1‑150 (MQ=255)
                                                                                tGCTTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCtttttt                                                           <  2:53158/146‑1 (MQ=255)
                                                                                 gCTTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTGTCTTTATTATCAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCttttttt                                                          <  2:55979/146‑1 (MQ=255)
                                                                                                    gTACAGACCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAatg                                       <  2:145076/146‑1 (MQ=255)
                                                                                                                        tGTCTTTATTATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCa               <  1:215653/150‑1 (MQ=255)
                                                                                                                               attatAAATTTCAGCTGCTTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCt            <  2:183755/146‑1 (MQ=255)
                                                                                                                               attatAAATTTCAGCTGCTTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCt            <  2:183848/146‑1 (MQ=255)
                                                                                                                                 tataAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATTTaa          >  2:188755/1‑146 (MQ=255)
                                                                                                                                     aaTTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACCGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATCAAtgtg  >  1:76046/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
CGTTGATTTGGGTTTCACCTTTGAAGCCAAGACGGGCATAAGTAGTATCACCATCATCTGCATCATTAGAGGAGAAGTAGTGCTTAGCATTAACTTTCCCGTACAGATCCAGCTTGTTACTGTCTTTATTATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATCAATGTG  >  CP009273/572071‑572353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: