| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,051,591 | G→T | 69.1% | intergenic (‑8/+93) | asnW ← / ← yeeO | tRNA‑Asn/putative multdrug exporter, MATE family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,051,591 | 0 | G | T | 69.1% | 6.1 / 6.7 | 13 | intergenic (‑8/+93) | asnW/yeeO | tRNA‑Asn/putative multdrug exporter, MATE family |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (4/5); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01 | |||||||||||
TTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGG > CP009273/2051464‑2051727 | tttAACCGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATg < 1:31144/150‑1 (MQ=255) aTGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTGGCGATGCCCACCCACAATGTCAAAGCCTGtt < 1:137902/150‑1 (MQ=255) ctcAAAACTAAAATTTGGCTCCTCTGACTTGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGtttttt > 1:140962/1‑150 (MQ=255) aaaaaCTAAAATTTGGCTCCTCGGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGtttt < 2:117250/145‑1 (MQ=255) gAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACGACAGAGGCATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAAGCGTTTGCTGAAATAATCTGCAttt < 1:195201/150‑1 (MQ=255) ccAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGACTCATGATAACGAGGCGAATGTTAGCGATGCCCACACACAAAGTCAAAGCCTGTGTTTTAAATTTGAAATCGTTTGCTGGAATAATCTGCAtt > 2:17930/1‑146 (MQ=25) aaCAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCgcc > 2:80441/1‑144 (MQ=255) cgccgTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACCCAACTGGCtt > 1:66011/1‑150 (MQ=255) gTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCtttttt < 1:161985/150‑1 (MQ=255) cTGAACTACAGAGGAATCGTGATACCGAGGCGAATATTAGCGAGGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACtttt < 1:137653/150‑1 (MQ=255) aCTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACtttt < 2:167725/146‑1 (MQ=255) tACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTgggggt > 1:45126/1‑148 (MQ=255) gAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACGGGCTTTTCTTCACCCTTTTCCGACTCgc > 2:19186/1‑145 (MQ=255) | TTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGG > CP009273/2051464‑2051727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |