Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,050,838	A→T	V186E (GTA→GAA)	glnA ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,050,838	0	A	T	100.0%	236.4 / NA	73	V186E (GTA→GAA)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (36/37);  total (36/37)

GTCAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGAAACGGAT  >  CP009273/4050693‑4050980                                                                                                                                                  |                                                                                                                                               gTCAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCg                                                                                                                                             <  1:1266193/149‑1 (MQ=255)   cAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGt                                                                                                                                           <  1:890320/149‑1 (MQ=255)      cTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGa                                                                                                                                        >  1:1221652/1‑149 (MQ=255)        tttttGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCCGAGCCGAGTCTTCCgg                                                                                                                                            >  2:596733/1‑143 (MQ=255)          tttGGTCATGGTTTTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCCCCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTg                                                                                                                                          <  2:917395/143‑1 (MQ=255)          tttGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCg                                                                                                                                    <  1:525097/149‑1 (MQ=255)          tttGGGCAGGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCACCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCAGCACAAGACTCATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCg                                                                                                                                    <  1:332467/149‑1 (MQ=255)           ttGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCgg                                                                                                                                   >  1:892174/1‑149 (MQ=255)           ttGGTCATGGTATTGAAGCGGGTACCCACTTCGTTCTGACCAGCAGTGGCTGCTTCGTGTTTATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTgg                                                                                                                                         <  2:490115/143‑1 (MQ=255)              gTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGaa                                                                                                                                <  1:964531/149‑1 (MQ=255)              gTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGaa                                                                                                                                <  1:446917/149‑1 (MQ=255)              gTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACGTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGaa                                                                                                                                <  1:701932/149‑1 (MQ=255)               tCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAAcc                                                                                                                                     >  2:1215132/1‑143 (MQ=255)                  tGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCCGCAGTAGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATCTCAGACCGACTATCCTGAGCCGAGTCTTCCGATGGAACCggg                                                                                                                                  >  2:51291/1‑143 (MQ=255)                  tGGTATTGAAGCGGGTAGCCACTGCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACACTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCggg                                                                                                                                  >  2:947099/1‑143 (MQ=255)                   ggTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAAc                                                                                                                           <  1:740436/149‑1 (MQ=255)                         gAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCtt                                                                                                                     <  1:479694/149‑1 (MQ=255)                             cGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACACATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGGGCCGAGTCTTCCGGTGGAACCGGGAAGCAACCGcc                                                                                                                       >  2:867959/1‑143 (MQ=255)                                gTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGCAACATTTCAGAACGAACAGCCTGACCCGAGTCTTCCGGTGGAACCGGGAAGGAACCGCCtgt                                                                                                                    >  2:149460/1‑141 (MQ=255)                                gTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGc                                                                                                              <  1:1458744/149‑1 (MQ=255)                                    ccACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCATGAGCCGAGTCTTCCGGTGGAACCGGGAAGGAACCGCCTTTCACTGCCGGa                                                                                                          >  1:717120/1‑149 (MQ=255)                                     cACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGAc                                                                                                         >  1:241597/1‑149 (MQ=255)                                      aCTTCGGTGTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGCAACGCCGTTCCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACg                                                                                                        <  1:719692/149‑1 (MQ=255)                                       cTTCGTTCTGACCAGCAGTCGCGACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGcc                                                                                                             <  2:1051384/143‑1 (MQ=255)                                               tGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATAACCTAAGCCGAGTCTTACGGTGGAACCGGGAAGTAACCGCATTTCAATGCGGGACGGTGACCttt                                                                                               >  1:868209/1‑149 (MQ=255)                                               tGACCAGCAGGCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCttt                                                                                               <  1:1271037/149‑1 (MQ=255)                                                 aCCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCGGGACGGCGAc                                                                                                   >  2:1147648/1‑143 (MQ=255)                                                  ccAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGGCTTTCACTGCCGGACGGCGAcc                                                                                                  >  2:816438/1‑143 (MQ=255)                                                   cagcagTCGCTACTTCGTGGTGATGGGCCTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTa                                                                                           <  1:946452/149‑1 (MQ=255)                                                     gcagTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGGGCAGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGGCGGTGCCATATGTTgcc                                                                                         >  1:239324/1‑149 (MQ=255)                                                       agTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCCTCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACCGCCGGACGGTGACCTTTGTTaccac                                                                                       <  1:750459/149‑1 (MQ=255)                                                           gCTCCTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTc                                                                                   <  1:303692/149‑1 (MQ=255)                                                           gCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAGCCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTacc                                                                                         >  2:1021380/1‑143 (MQ=255)                                                            cTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGATCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTacca                                                                                        <  2:1187381/143‑1 (MQ=255)                                                            cTACCTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTacca                                                                                        <  2:232745/143‑1 (MQ=255)                                                               cTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTAt                                                                               <  1:656956/149‑1 (MQ=255)                                                                    tggtgATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCAGAGCCGAGTCTTCCGGTGCAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACATTCGGATTCGGt                                                                          >  1:318319/1‑149 (MQ=255)                                                                     ggggATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTAt                                                                               <  2:774492/140‑1 (MQ=255)                                                                       tgttGGGCTTCAACCCCCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTg                                                                             <  2:168372/140‑1 (MQ=255)                                                                       tgATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTgga                                                                       <  1:811221/149‑1 (MQ=255)                                                                        gATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTgg                                                                            <  2:1038834/143‑1 (MQ=255)                                                                        gATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTggag                                                                      >  1:586055/1‑149 (MQ=255)                                                                         aTGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTggg                                                                           <  2:1148255/143‑1 (MQ=255)                                                                              cTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTggag                                                                      <  2:36691/143‑1 (MQ=255)                                                                               ttCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCCCTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTggagc                                                                     >  2:1097065/1‑142 (MQ=255)                                                                                  aaCCACCAGACCGATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGt                                                                  <  2:370293/143‑1 (MQ=255)                                                                                  aaCCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACAGCCTTTCACTGCCGGACGGTGACCTTGGTTCCCGCCTTCCCACTGGGCggaagatg                                                                  >  2:1098933/1‑141 (MQ=255)                                                                                   accaccAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGGCGGTGGCCTTTGTTACCACCTTAGTTTTGGGTGGGGGGGTTCCGTgg                                                           >  1:813502/1‑148 (MQ=255)                                                                                   accaccAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATgc                                                           >  1:1364791/1‑149 (MQ=255)                                                                                     ccccaGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTcc                                                               <  2:323881/141‑1 (MQ=255)                                                                                      accaGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCa                                                              >  2:296533/1‑143 (MQ=255)                                                                                        caGCCCCATCTGTTCCATCGCCAGGCACATTTCAGCACGAGTATCCTGAGCCGAGTCTTCCGGTGGATCCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTCTTACCACCTTCGTATTGGGTGGAGGAGTTCCATg                                                            <  2:167816/143‑1 (MQ=255)                                                                                         ggACCCATCTGTCCCATCATCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTc                                                     <  1:1496187/148‑1 (MQ=255)                                                                                              cATCTGTTCCATCACCAGACACATTTCAGAACATCGTCCCGGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGatat                                                >  1:1481522/1‑149 (MQ=255)                                                                                                  tGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGat                                                  <  2:80231/143‑1 (MQ=255)                                                                                                    ttCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGCTCCATGCGCCTCCGatat                                                >  2:1153922/1‑143 (MQ=255)                                                                                                      ccATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATa                                        <  1:1285614/149‑1 (MQ=255)                                                                                                       cATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGGAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAg                                       >  1:1446990/1‑149 (MQ=255)                                                                                                             cAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGAGGGAGGAGTTCCATGCGCCTTCGATATCGTCGAAAg                                       >  2:70780/1‑143 (MQ=255)                                                                                                               gACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTgg                               <  1:1399381/149‑1 (MQ=255)                                                                                                               gACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTgg                               <  1:1398823/149‑1 (MQ=255)                                                                                                               gACACATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTgg                               <  1:1343069/149‑1 (MQ=255)                                                                                                                acacATTTCAGAACGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGGTCCATGCGCCTTCGATCTCGTCGATAGGAACGTggg                              >  1:1472128/1‑149 (MQ=255)                                                                                                                          gcaCGAACTTCCTGAGGCGAGTCTTCGGGGGGTACCGGGAAGTAACCGCCTTTCACTGCCGGAGGGTGAGCTTTGTTACCAGCTTCGTATTGGGTGGGGGAGTTCGATGCGCCTTCGATATCGTCGTTGGCGAGGTGGGGAcc                          >  2:913461/3‑143 (MQ=255)                                                                                                                           aaCGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGGTATCGTCGATAGCAACGTGGGACCCGgagaga                   >  1:851719/1‑147 (MQ=255)                                                                                                                           aaCGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATGGCAACGTGGGAACCGgagaga                   >  1:91650/1‑147 (MQ=255)                                                                                                                            aCGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTACCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGACAGCAACGTGGGAACCGGagatag                  >  1:1154992/1‑149 (MQ=255)                                                                                                                            aCGAATATCCTGAGCCGAGTCCTCCGCAGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGCCCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCCTGCGCCTTCGATATCGTCGATAGCAACGTGGGTAACgg                        >  2:498145/1‑143 (MQ=255)                                                                                                                             cGAATATCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGGAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGagataga                 <  1:1151476/149‑1 (MQ=255)                                                                                                                                 tatCCTGAGCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGagata                   >  2:546965/1‑143 (MQ=255)                                                                                                                                         gCCGAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATGGATGTTCCGAAACg     >  1:5072/1‑149 (MQ=255)                                                                                                                                            gAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGaa        >  2:791590/1‑143 (MQ=255)                                                                                                                                            gAGTCTTCCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCAACTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGAGACGAGGGAACAGGAGCTAGATGATCCGAAACGGAt  >  1:1002863/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                               GTCAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGAAACGGAT  >  CP009273/4050693‑4050980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑