Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,884,224 G→A T189I (ACA→ATA)  yeaY ← Slp family lipoprotein, RpoE‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,884,2240GA97.9% 275.9 / NA 95T189I (ACA→ATA) yeaYSlp family lipoprotein, RpoE‑regulated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (41/51);  minor base C (2/0);  total (43/52)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTA  >  CP009273/1884080‑1884368
                                                                                                                                                |                                                                                                                                                
catcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGAGCGTCACTATTTATTTTTATATTTACCGTAAGAATGCAGAAACGGCGGACCAGCCGCTGTTTCTTTTTTTTTAAAAACACGCATTGATTAAGTTACAACTAttta                                                                                                                                              >  1:813093/1‑148 (MQ=255)
 atcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGt                                                                                                                                             <  1:565019/149‑1 (MQ=255)
  tcatACCAGCTGGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTACTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTa                                                                                                                                            <  2:1705403/149‑1 (MQ=255)
   catACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTAc                                                                                                                                           >  2:672509/1‑149 (MQ=255)
    atACCAGCTTGATTATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATATATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTAct                                                                                                                                          >  2:1329492/1‑149 (MQ=255)
      aCCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTActct                                                                                                                                        <  2:1035029/149‑1 (MQ=255)
         aGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTATTTCTTTGTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTcgc                                                                                                                                     >  2:1435162/1‑149 (MQ=255)
          gCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTcgcg                                                                                                                                    >  1:1123518/1‑149 (MQ=255)
          gCTGGATAATTACCCAACTAAAAGGGTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCACTTACAACTATTTGTACTCTcgcg                                                                                                                                    <  1:119310/149‑1 (MQ=255)
           cTTGTTAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCgg                                                                                                                                   <  1:1338826/149‑1 (MQ=255)
           cTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCgg                                                                                                                                   <  2:1845654/149‑1 (MQ=255)
            ttGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCggg                                                                                                                                  >  1:355470/1‑149 (MQ=255)
             tGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCgggg                                                                                                                                 >  2:1371011/1‑149 (MQ=255)
              gATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTCCTCTCGCGGGGc                                                                                                                                >  1:1013668/1‑149 (MQ=255)
              gATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGc                                                                                                                                >  1:147626/1‑149 (MQ=255)
                tAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTCCAACTATTTGTACTCTCGCGGGGCCg                                                                                                                              >  2:158098/1‑149 (MQ=255)
                   ttACCCAACGAACAGGTTGCGAAGCGCGCCACGATTTATTTTTATCTGTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGa                                                                                                                           <  1:653929/149‑1 (MQ=255)
                   ttACCCAACGAAAAGGTTGCGAAGCGCGGCACAATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTATTTCTTTTTCTTAAAAAACAAGCAATTATTCAGGTACACCTATTTGTACTCCCGCGGGGCCGGGa                                                                                                                           >  1:393551/1‑149 (MQ=255)
                     aCCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCATTTACAACTATTTGTATTCTCGCGGGGCCGGGAtt                                                                                                                         >  2:800528/1‑149 (MQ=255)
                     aCCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGact                                                                                                                         >  2:705825/1‑147 (MQ=255)
                     aCCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGGtt                                                                                                                         >  2:1715321/1‑149 (MQ=255)
                     aCCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCCCCGGGCCGGGCtt                                                                                                                         >  1:1561864/1‑149 (MQ=255)
                       ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATtat                                                                                                                       >  2:989473/1‑149 (MQ=255)
                        cAACGAGAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATtata                                                                                                                      <  2:1077333/149‑1 (MQ=255)
                        cAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCTGGTATaatc                                                                                                                      >  2:223032/1‑148 (MQ=255)
                         aaCGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTCTTTGTACTCTCGCGGGGCCGGGGTTATAc                                                                                                                     >  1:516705/1‑149 (MQ=255)
                         aaCGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAAGTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGGTTGTAc                                                                                                                     >  1:1477276/1‑149 (MQ=255)
                            gAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAg                                                                                                                  >  2:555932/1‑149 (MQ=255)
                            gAAAAGGTTGCGAAGCGCATCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTGTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAg                                                                                                                  >  2:1424178/1‑149 (MQ=255)
                              aaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGcc                                                                                                                <  2:1937842/149‑1 (MQ=255)
                              aaaGGTTGCGAAGCGCGTCACTATTTACTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATACAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGcc                                                                                                                <  2:895404/149‑1 (MQ=255)
                                  gTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                                                                            <  2:1771548/149‑1 (MQ=255)
                                      cGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTCTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgc                                                                                                        >  2:585138/1‑149 (MQ=255)
                                          gcgcGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTa                                                                                                    <  1:1555561/149‑1 (MQ=255)
                                           cgcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAg                                                                                                   >  2:154095/1‑149 (MQ=255)
                                            gcgTCACTTTTTATTTTTTTCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGc                                                                                                  <  1:612770/149‑1 (MQ=255)
                                             cgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGcc                                                                                                 >  2:595999/1‑149 (MQ=255)
                                               tCACTATTTATTTTTACCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCAt                                                                                               <  2:1747370/149‑1 (MQ=255)
                                               tCACCATTTATTTTTATCTTTACCTTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACCAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCAt                                                                                               <  1:637564/149‑1 (MQ=255)
                                                 aCTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAg                                                                                             <  2:1527017/149‑1 (MQ=255)
                                                      ttatttTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgcca                                                                                        <  1:1399352/149‑1 (MQ=255)
                                                      tcacttttATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCGGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgcca                                                                                        <  1:1382766/145‑1 (MQ=255)
                                                         tttttATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTGTTGTCTCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgccagcc                                                                                     >  2:1896415/1‑149 (MQ=255)
                                                             tATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGa                                                                                 <  2:24290/149‑1 (MQ=255)
                                                               tCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGAcc                                                                               <  2:385347/149‑1 (MQ=255)
                                                                cTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCg                                                                              <  2:597070/149‑1 (MQ=255)
                                                                cTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCg                                                                              <  1:130477/149‑1 (MQ=255)
                                                                 tttACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCACCCACGACCGc                                                                             >  1:462877/1‑149 (MQ=255)
                                                                  ttACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGcc                                                                            >  1:1700701/1‑149 (MQ=255)
                                                                  ttACCGCAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGcc                                                                            <  2:572576/149‑1 (MQ=255)
                                                                     ttttaaagaaaaaGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTAAGTTACAAGTATTTGTACTCTCGCGGGGCCGGGATTATCCCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCAtg                                                                         >  2:1712071/13‑148 (MQ=37)
                                                                      cGTAAGAATGCAGAAACAGCGGACCAGCCTGTGGTTCTTTTTATTCCAAAACAATCGATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATaa                                                                        <  2:1891159/149‑1 (MQ=255)
                                                                      cGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATaa                                                                        <  2:1651358/149‑1 (MQ=255)
                                                                       tccgatctGCAGAAACAGCGCACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATCCAGTTACACCTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATaaa                                                                       <  2:1618895/142‑1 (MQ=255)
                                                                       gTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCCGCCCCAGCCGCCGTACCCATAGGGCCAGCCACGACCGCCATaaa                                                                       >  2:1013437/1‑149 (MQ=255)
                                                                        caagatntcAGACACGGCGGACCAGCCGCTGTTTTTNTTTCTTTAAAAACAAGCACTTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGCCCGCCATaaaa                                                                      <  1:1730222/141‑1 (MQ=255)
                                                                         aaGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                                                                     <  1:997463/149‑1 (MQ=255)
                                                                           gAATGAAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCa                                                                   >  1:742993/1‑149 (MQ=255)
                                                                            aaTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCAt                                                                  >  2:1864536/1‑149 (MQ=255)
                                                                            aaTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCAt                                                                  <  2:1726137/149‑1 (MQ=255)
                                                                                cAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTCCAACTATTTGTACGCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGAt                                                              <  2:1481776/149‑1 (MQ=255)
                                                                                cAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATaaaaagatcgga                                                              >  1:1499420/1‑141 (MQ=255)
                                                                                cAGAAACAGCGGACCACCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGAt                                                              <  2:900833/149‑1 (MQ=255)
                                                                                   aaacagcggctggtccGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCaa                                                           >  2:1132431/15‑149 (MQ=255)
                                                                                     acagctcaacacccGCTTTTTCTTTTTCTTCAAAAACAAGCAATTATTCAGTTACAACTATCTGTACTCTCGCGGGGCCGGGATTATCCCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATc                                                         <  2:94725/137‑1 (MQ=255)
                                                                                       ggCGGACCAGCCGCTTTTTCTTTTTCTTTAAAAACAAGCAATTACTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCgg                                                       <  1:1545535/148‑1 (MQ=255)
                                                                                         cGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCACCCACGACCGCCATAAAACCATGGATCAATCGGCt                                                     >  2:46925/1‑149 (MQ=255)
                                                                                             ccAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAgg                                                 <  1:1313159/149‑1 (MQ=255)
                                                                                                gCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACACCTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAggcgg                                              <  2:449901/149‑1 (MQ=255)
                                                                                                gCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAggcgg                                              <  2:773583/149‑1 (MQ=255)
                                                                                                 gcGCTTTCTCTTTTTTTTAAAAACCAAGCAATTATTCAGTTACACCTATTTGTCCTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGCCCGCCATAACACCATGGATCAATCGGCTGAggcggc                                             <  1:433212/148‑1 (MQ=255)
                                                                                                    cTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATa                                          >  1:956794/1‑149 (MQ=255)
                                                                                                      gtgtgttgtcctttAAAAACAAACAATTATTCAGTTACAACTTTTTGCACTCTCGCGGGGCCGGGATTATACCACCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAAt                                        <  1:272106/139‑1 (MQ=255)
                                                                                                       tttcttgttcttTAAAAACAAGCAATTATTCAGTTACAACTATGTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATc                                       <  2:332478/149‑1 (MQ=255)
                                                                                                         tttttttctttAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCAc                                     <  1:643392/147‑1 (MQ=255)
                                                                                                         tctttttctttAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGCCCGGGATTATACCAGCCCCATCCGCCGTAGCCAGAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCAc                                     <  2:564717/149‑1 (MQ=255)
                                                                                                         tctttttcttcACAAACAAGCAATCATTCAGTTACAAATAAAAGTTAAATCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCAc                                     <  2:1595402/149‑1 (MQ=255)
                                                                                                           tttttctttAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGCCTGAGCCGCCATAACCACct                                   >  1:1209748/1‑149 (MQ=255)
                                                                                                            ttttctttAAAAACAAGCACTGATTCAGTTACAACTATTGGTACCCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACctt                                  <  1:897265/149‑2 (MQ=255)
                                                                                                              tcttttAAAAACAAGCAATTATTCAGTTCCAACTATTTGTACTCGCGCGGGGCCGGAATTATACCCGCCCCATCCGCCTTAGCCACAGGGCCAGCCACGACCGCCATAAAACCATTGATCAATCGGCTGAGGCGGCATAATCACctgct                                <  1:623710/146‑1 (MQ=255)
                                                                                                                ttttAAAAACAAGCAATTATTCCGTTACAACTATTTGTACTCTCGGGGGGCGGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTgg                              <  1:159474/148‑1 (MQ=255)
                                                                                                                ctttAAAAACAGGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGGCGGGATTATACCGGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTgg                              <  1:555483/149‑1 (MQ=255)
                                                                                                                 tttAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTggg                             >  2:973727/1‑149 (MQ=255)
                                                                                                                 tttAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCGGCTggg                             >  1:623884/1‑149 (MQ=255)
                                                                                                                 tatAAAAACAAGCAATTATTCAGTTACAACAATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTggg                             <  2:1001270/147‑1 (MQ=255)
                                                                                                                  ttAAAAACAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGt                            >  2:1697603/1‑149 (MQ=255)
                                                                                                                   aaaaaaaACGTAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGtt                           <  1:863233/148‑1 (MQ=255)
                                                                                                                       aaCAAGCAATTATACAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAAt                       >  1:1238988/1‑149 (MQ=255)
                                                                                                                         cAAGCAATTATTCAGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGc                     <  2:502573/149‑1 (MQ=255)
                                                                                                                           aGCAATTATTCAGTGACACCTATTTGCACTCCCGCGGGGCCGGGATTATACCAGCCCAATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCa                   <  2:1606501/149‑1 (MQ=255)
                                                                                                                            gaaaTTATTCGGTTACAACTATTTGTACGCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCaa                  <  1:920113/147‑1 (MQ=255)
                                                                                                                                  attCAGTTACAACCATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTg            <  1:613747/149‑1 (MQ=255)
                                                                                                                                      aGTTACAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAAc        >  2:574284/1‑149 (MQ=255)
                                                                                                                                           cAACTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGtt   >  1:92741/1‑149 (MQ=255)
                                                                                                                                            aaCTATTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATCAAACCATGGATCACTCGGCTGAGGCGGCATAATCAACTGCTGGGCTAAATGCGAACGTTTCTAACCCGTTa  >  1:783414/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTA  >  CP009273/1884080‑1884368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑