Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,051,591 G→T intergenic (‑8/+93) asnW ← / ← yeeO tRNA‑Asn/putative multdrug exporter, MATE family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,051,5910GT99.1% 359.3 / NA 109intergenic (‑8/+93)asnW/yeeOtRNA‑Asn/putative multdrug exporter, MATE family
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (56/52);  total (57/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TAAAAATAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGT  >  CP009273/2051446‑2051736
                                                                                                                                                 |                                                                                                                                                 
tAAAAATAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTTCCGACTGAACTACAGAGGAATCGTGATAAc                                                                                                                                                <  2:1017652/149‑1 (MQ=255)
 aaaaaGAATTTTTATTTTTTAAAGGTTTTGAGGCTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACg                                                                                                                                               <  2:834192/149‑1 (MQ=21)
      taatTTTTATTTTTTAACTGTTTTGATACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATCAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCg                                                                                                                                          <  1:949682/149‑1 (MQ=255)
       aatTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGa                                                                                                                                         <  2:1602482/149‑1 (MQ=255)
        atttatatttttTAACTGATTGGAGACTCATAGAGATGTCTTAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGaa                                                                                                                                        <  2:1878256/149‑1 (MQ=18)
         tttttatttttTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAat                                                                                                                                       <  2:854336/149‑1 (MQ=255)
         ttcttatttttTAACTTTTCTGCGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAat                                                                                                                                       <  1:1629126/146‑1 (MQ=18)
          ttttatttttTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCACCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATCACGAGGCGAatg                                                                                                                                      >  2:859843/1‑148 (MQ=255)
            ttatttttTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACGACAGAGGAATCGTGATAACGAGGCGagagtt                                                                                                                                    >  1:1191637/1‑144 (MQ=255)
               ttttttAACTGTTTTGAGACTCATAGAGCTGTCTCAAAACGAAGCTTAGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAAGCGTGATAACGAGGCGAATATTAGc                                                                                                                                 <  2:637837/149‑1 (MQ=18)
               ttttttAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGc                                                                                                                                 >  2:508178/1‑149 (MQ=255)
               ttttttAACTGTTTGGAGACTCATAAAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGTGGAATCGTGATAACGAGGCGAATATTAGc                                                                                                                                 <  2:1180425/149‑1 (MQ=255)
                        tGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGcccac                                                                                                                        >  2:1658965/1‑149 (MQ=255)
                         ggtttGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCCGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGcccacc                                                                                                                       <  2:771470/147‑1 (MQ=255)
                          ttttGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATacccaccc                                                                                                                      >  1:368634/1‑149 (MQ=255)
                           tttGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAACGATGcccaccca                                                                                                                     >  1:568664/1‑149 (MQ=255)
                               agaCTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAAt                                                                                                                 >  1:1462460/1‑149 (MQ=255)
                               acaCTCATAGAGGTGTCTCAAAACTACAACTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAAt                                                                                                                 <  1:373844/147‑1 (MQ=255)
                                 aCTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGt                                                                                                               <  1:1411721/149‑1 (MQ=255)
                                 aCTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGt                                                                                                               >  2:1586009/1‑149 (MQ=255)
                                 aCTCAGAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGt                                                                                                               <  2:231480/149‑1 (MQ=255)
                                     aTAGGGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCgaa                                                                                                           >  2:1530444/1‑149 (MQ=255)
                                     aTAGAGATGTCTCACAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCaaa                                                                                                           <  2:769305/149‑1 (MQ=255)
                                     aTAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAAGGAGGCGAATATTAGGGGTGCGCACCCCCAATGTGaaa                                                                                                           >  2:484324/1‑149 (MQ=18)
                                     aTAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCaaa                                                                                                           >  1:1704795/1‑149 (MQ=255)
                                       agagATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGATACCCGAGTTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGTCCACCCACAATGTCAAAGc                                                                                                         <  2:1412217/149‑1 (MQ=21)
                                                tcAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGtttttt                                                                                                >  2:871896/1‑149 (MQ=255)
                                                tcAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGAGTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGtttttt                                                                                                >  2:509556/1‑149 (MQ=255)
                                                 cAAAACTAAAATTTGGCTCCCCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTa                                                                                               <  2:244810/149‑1 (MQ=255)
                                                    aaCTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATCCGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGTATCGTTATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAAt                                                                                            <  2:1419966/149‑1 (MQ=255)
                                                      cTATAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGCTTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAAGGAGGCGAATATTAGCGATGCCCACCCACAATGTAAAAGCCTGGTTTTTAAAttt                                                                                          >  1:314454/1‑149 (MQ=18)
                                                      cTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACGACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAAttt                                                                                          <  2:1871011/149‑1 (MQ=255)
                                                       tAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGGCTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTg                                                                                         >  1:1437289/1‑149 (MQ=255)
                                                       tAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATGGTGATAACGAGGCGAATATTAGCGATGCCCACCCACGATGTCAAAGCCTGTTTTTTAAATTTg                                                                                         >  2:707527/1‑149 (MQ=255)
                                                         aaaTTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGaa                                                                                       <  2:1071073/149‑1 (MQ=255)
                                                          aaTTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGaaa                                                                                      >  2:944343/1‑149 (MQ=255)
                                                          aaTTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCCTGGGATCCAGGTGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGaaa                                                                                      >  2:1414035/1‑149 (MQ=21)
                                                            tttGGCGCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATc                                                                                    <  1:531701/149‑1 (MQ=255)
                                                            gttGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTTAATTCGaaaga                                                                                    >  2:1930084/2‑147 (MQ=255)
                                                              gggCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACGACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGt                                                                                  <  1:1048309/148‑1 (MQ=255)
                                                                 ctcctcTGACTGGGCTCGAATCAGTGACATACGGATTAACAGTCCGCCGCTCTACCGACGGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTg                                                                               <  2:73921/149‑1 (MQ=25)
                                                                 ctcctcTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCGTGTTTTTTAAATTTGAAATCGTTTg                                                                               <  2:1156093/149‑1 (MQ=255)
                                                                  tcctcTGACTGGACTCGGACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTTAACTACAGAGGAATCGTGATAACGAGGCGAATATTCGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGc                                                                              >  1:1323200/1‑149 (MQ=255)
                                                                  tcctcTGACTGGACTCGAACCAGTGACATCCGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGc                                                                              <  2:399958/149‑1 (MQ=255)
                                                                  tcctcTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGAATGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGc                                                                              >  2:939147/1‑149 (MQ=255)
                                                                           tGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTCCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAaataat                                                                     <  1:1605798/149‑1 (MQ=255)
                                                                            ggacacgccccAGTCACCTACGACTTCACAGTCCGCCGTGTTACCGACCGAACTACAGACGACTCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATc                                                                    <  2:887122/140‑1 (MQ=255)
                                                                              aCTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTg                                                                  >  2:509862/1‑149 (MQ=255)
                                                                               cTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGc                                                                 >  1:235279/1‑149 (MQ=255)
                                                                                 cGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATATGAAATCGTTTGCTGAAATAATCTGCAt                                                               <  2:1035727/149‑1 (MQ=255)
                                                                                  gAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGCAGTCATCTGCAtt                                                              >  2:618366/1‑149 (MQ=255)
                                                                                  gAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCAtt                                                              <  2:1728234/149‑1 (MQ=255)
                                                                                          gACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGtt                                                      >  2:493327/1‑149 (MQ=255)
                                                                                            cATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTa                                                    >  2:1149761/1‑149 (MQ=255)
                                                                                              tACGGATTAACTGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTAtt                                                  <  1:1124948/149‑1 (MQ=255)
                                                                                              tACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTAtt                                                  <  2:1121960/149‑1 (MQ=255)
                                                                                               aCGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTc                                                 >  2:1223364/1‑149 (MQ=255)
                                                                                                cGGATTAACAGTCCGCCGTTCTACCGACTGAACCACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTcc                                                <  1:1238025/149‑1 (MQ=255)
                                                                                                 ggATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCg                                               <  2:164853/149‑1 (MQ=255)
                                                                                                 ggATTAACAGGCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCg                                               <  1:1563209/149‑1 (MQ=255)
                                                                                                  gATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGa                                              <  1:791646/149‑1 (MQ=255)
                                                                                                  gATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATATGCATTTTGTCGTTTATTCCGa                                              >  2:943382/1‑149 (MQ=255)
                                                                                                       aCAGTCCGCCGTTCTACCGACTGAACTAGAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACaa                                         >  2:1261698/1‑149 (MQ=255)
                                                                                                         aGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGATATAGTCTGCATTTTGTCGTTTATTCCGACACCACt                                       >  2:46175/1‑149 (MQ=255)
                                                                                                         aGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTATTCCGAGATAATCTGCATTTTGTCGTTTATTCCGACACAACt                                       >  1:1721340/1‑149 (MQ=38)
                                                                                                           tCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAAcgcg                                     >  1:1273565/1‑146 (MQ=255)
                                                                                                             cgccgTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCATAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTCGTCGTTTATTCCGACACAACTGGCt                                   >  2:1196496/1‑149 (MQ=255)
                                                                                                             cgccgTTCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCt                                   >  2:1058267/1‑149 (MQ=255)
                                                                                                              gccgTTCTACCGACTGAACTACAGAGGAATCATGATAACGAGGCGAATATTAGCGATGCCCACCCCCAATGCCAAAGCCTGGTTTTTAAATTTGAAATCGTTTGCTGAAAAAATCTGCATTTTGTCGTTTATTCCGCCACACCCGGCtt                                  >  1:778971/1‑149 (MQ=38)
                                                                                                                  tttcaCCGCCTGAACTACGGAGTAATCGTGATAACGAGGCTAAGATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGAATGCTTTCGTTTATTCCGACACAACTGGCtttttt                              <  1:1735007/145‑1 (MQ=255)
                                                                                                                  ttCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCtttttt                              >  1:407821/1‑149 (MQ=255)
                                                                                                                  ttCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCtttttt                              <  1:310025/149‑1 (MQ=255)
                                                                                                                  ttCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCtttttt                              <  1:310701/149‑1 (MQ=255)
                                                                                                                   tgcaCCGACGGAACTACAGAGGAGTCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAACTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCttttttt                             <  2:1656074/146‑1 (MQ=255)
                                                                                                                   tCTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGGCGTTTATTCCGACACAACTGGCttttttt                             <  2:1187708/149‑1 (MQ=255)
                                                                                                                    cTACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTc                            <  1:666486/149‑1 (MQ=255)
                                                                                                                     tACCGACTGCACTACAGCGGAAGCGTGATAACGAGGAGAAGATTAGCGATGCCCACCCACAATGTCAAAGCCCGCTTTTTAAATTTGAAATCGTTTGCTGAAAGAATCTGCATCTTGTCGTTTATTCCGCCACAACCGGCCTTTTTTca                           >  1:859994/1‑149 (MQ=255)
                                                                                                                     tACCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTca                           <  2:1826478/149‑1 (MQ=255)
                                                                                                                      aCCGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTcac                          >  2:1860076/1‑149 (MQ=255)
                                                                                                                       ccGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAATTTTGAAATCGTTTGCAGAAATAAACTGCATTTTGTCGTTAATTCCTACACAGCACGCttttctcctcg                         >  1:1652045/1‑142 (MQ=255)
                                                                                                                       ccGACTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTcaca                         >  1:1118255/1‑149 (MQ=255)
                                                                                                                       acgtcTGAACGACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTcaca                         <  2:1792403/145‑1 (MQ=255)
                                                                                                                         gACTGAGCTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTTTTTTTTTAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACCCt                       >  1:823052/1‑149 (MQ=255)
                                                                                                                          aCTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACtt                      >  2:346717/1‑149 (MQ=255)
                                                                                                                           cTGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATCCGGAACCAGAATGTGAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTAGTTTATTCCGCCACAACTGGCTTTTTTTCACACttt                     >  1:1639164/1‑149 (MQ=255)
                                                                                                                            tGAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACtctt                    >  1:29188/1‑149 (MQ=255)
                                                                                                                             gAACTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCGTTTTGTCGTTTATTCCGACGCAACTGGCTTTTTTTCACACTTTTg                   >  2:558383/1‑149 (MQ=255)
                                                                                                                              aaCTACAGAGGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGc                  <  1:1612827/149‑1 (MQ=255)
                                                                                                                                    agagGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCg            >  1:1299712/1‑149 (MQ=255)
                                                                                                                                     gagGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTGATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCgg           <  1:1641068/149‑1 (MQ=255)
                                                                                                                                      agGAATCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCggg          >  1:315597/1‑149 (MQ=255)
                                                                                                                                          aTCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGa      <  1:963291/149‑1 (MQ=255)
                                                                                                                                          aTCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGa      <  2:543075/149‑1 (MQ=255)
                                                                                                                                          aTCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGa      <  2:43845/149‑1 (MQ=255)
                                                                                                                                          aTCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACAAAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGa      >  1:705918/1‑149 (MQ=255)
                                                                                                                                           tCGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAg     >  1:1460328/1‑149 (MQ=255)
                                                                                                                                            cGTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAgg    <  1:927703/149‑1 (MQ=255)
                                                                                                                                             gTGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGCTCGAggg   >  1:1634821/1‑149 (MQ=255)
                                                                                                                                              tGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGATCGAGGGt  >  1:972321/1‑149 (MQ=255)
                                                                                                                                              tGATAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTACTTTAATTTAACACAACTGGCTTTTTTTCACACTTTTTCGGCTCGGGTCGAGGGt  >  2:1405082/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TAAAAATAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGT  >  CP009273/2051446‑2051736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: