| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,598,477 | A→G | 18.2% | K103R (AAA→AGA) | hyfD → | hydrogenase 4, membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,598,477 | 0 | A | G | 18.2% | 41.2 / 4.8 | 22 | K103R (AAA→AGA) | hyfD | hydrogenase 4, membrane subunit |
| Reads supporting (aligned to +/- strand): ref base A (12/6); new base G (2/2); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01 | |||||||||||
AGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGG > CP009273/2598331‑2598609 | aGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTTATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATaaaa < 1:46504/149‑1 (MQ=255)aGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATaaaa < 1:229459/149‑1 (MQ=255) gttgGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCGGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATa > 1:352720/1‑149 (MQ=255) gTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTGGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCa > 2:328712/1‑143 (MQ=255) ttGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATc > 1:44841/1‑149 (MQ=255) tGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAGAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCg > 1:11298/1‑149 (MQ=255) tGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAGAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCg > 1:11289/1‑149 (MQ=255) gcgTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCGGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCAGAACGGCACGAACCGTTATTACGCATTTTTACTGGAGTTTATc > 2:145437/1‑143 (MQ=255) ggtgttgTTTCTCGGTTGGCTGGTCACGAGCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGa < 1:150626/149‑1 (MQ=255) tttCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGCCTGGTACTCTCCTCGAc > 2:60005/1‑143 (MQ=255) tttCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACgctgct > 1:124858/1‑149 (MQ=255) tGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACCCCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAg < 2:39944/143‑1 (MQ=255) ggTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTCCTCGCCTCGACCCCGCCCGGCCAgt > 2:194767/1‑143 (MQ=255) gTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAgttgttgtt > 1:236392/1‑149 (MQ=255) gTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAgttgttgtg > 1:94620/1‑148 (MQ=255) ccgatctCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGttt < 1:47146/143‑1 (MQ=255) ccgatccctccTCCATGGGTTATCTGACTGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGttt < 1:47077/139‑1 (MQ=255) cTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGGGTTTATCGGCGCGGTGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCGGTTGTTGtttt > 2:262899/1‑143 (MQ=255) tACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGGCGCTGCTCGGTCAGTTGTTGgtttg > 2:41996/1‑142 (MQ=255) tACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGtttta > 2:42063/1‑142 (MQ=255) ccACGGGTTATCTGACGGATAGAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGaa < 2:9896/143‑1 (MQ=255) ccACGGGTTATCTGACGGATAGAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGaa < 2:9904/143‑1 (MQ=255) cACGGGTAATCTTAACGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGCGTTTATCGGCTGGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGaaa < 2:118709/143‑1 (MQ=255) ggTTATCTGACGGATAAAAATCGCGAACCCCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAggcgg < 1:13716/149‑1 (MQ=255) | AGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGG > CP009273/2598331‑2598609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |