| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 877,748 | C→A | 15.1% | S148I (AGC→ATC) | deoR ← | deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 877,748 | 0 | C | A | 15.1% | 51.8 / 3.5 | 27 | S148I (AGC→ATC) | deoR | deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx |
| Reads supporting (aligned to +/- strand): ref base C (11/12); new base A (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
AGTGTTTTACCGGCAACTCTTCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGT > CP009273/877608‑877895 | aGTGTTTTACCGGCAACTCTTCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGa > 1:87266/1‑149 (MQ=255) ttACCGGCAACTCTTCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGATGGCGTGAAATTca > 1:156952/1‑149 (MQ=255) ttACCGGCAACTCTTCAAGATTAAAACAGGTAGCGCCTTTACTGACATGAACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGGTTGAAAATGG‑CGTTGATGGCGTGAAATTca > 1:196449/1‑149 (MQ=255) ggCAACTCGTCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTc < 2:217769/143‑1 (MQ=255) tctTCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAg < 1:366536/149‑1 (MQ=255) tcctCAAGATTAAGACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAg < 1:14144/146‑1 (MQ=255) ttCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGaa > 1:170879/1‑149 (MQ=255) gATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGACATAATCGGGTAGAAGTTATTCAGCGTTTGCTGACAATCGGTGGGTTTGAAAATGG‑CGGTGCTGGCGAGAGAATCACCGCCGCGTAGACAcgcg > 1:124153/1‑149 (MQ=255) ccTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTtctc < 1:145539/149‑1 (MQ=255) tcaccgACATGCCCGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATCATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCttt < 1:147933/144‑1 (MQ=255) tACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGACAGTGGACGAT‑CAGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTc > 1:86027/1‑149 (MQ=255) tGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAgc > 1:289301/1‑149 (MQ=255) tGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGCTTCTCTTTCAg > 2:245594/1‑143 (MQ=255) cgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGGTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAgg > 2:16797/1‑143 (MQ=255) gcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGt > 2:160076/1‑143 (MQ=255) cAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGc > 1:58559/1‑149 (MQ=255) cAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGc > 1:357947/1‑149 (MQ=255) ggCTAAAATTATTCAGCGGTTGCTGAAAATCGATGGGTTTGAAAAGGG‑CGTTGATGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAcggcg < 2:173295/143‑1 (MQ=255) ggCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGATGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAcggcg < 2:138532/143‑1 (MQ=255) gngaaaaTTATTCATCGTTTGCCGAAACTGGATTGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTGACAGCCGAACAGCGCCGGCTCTCAATTGGGTTTCTCGGTCAGCGCCAGAAAGGTACTTATCGAATAACAAAcggcgg < 2:318602/140‑1 (MQ=255) cAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAgg < 1:181855/149‑1 (MQ=255) ttCCGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCAtt < 1:172023/149‑1 (MQ=255) cAGCGTTTGCGGAAAATCGATGGGTTGTAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGAttt < 2:10188/143‑1 (MQ=255) gATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCaa < 2:77179/143‑1 (MQ=255) aaTGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCg > 2:219365/1‑143 (MQ=255) ggg‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggtggtg < 1:92266/148‑1 (MQ=255) gCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGt < 1:274044/149‑1 (MQ=255) | AGTGTTTTACCGGCAACTCTTCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGG‑CGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGT > CP009273/877608‑877895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |