Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,563,861 G→T 100% L296M (CTG→ATG)  gadC ← glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,563,8610GT100.0% 49.6 / NA 19L296M (CTG→ATG) gadCglutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/13);  total (6/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Not supported by required number of reads on both strands.

CCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCAGTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACA  >  CP009273/1563732‑1563997
                                                                                                                                 |                                                                                                                                        
ccGGTACGCCGTTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCAcg                                                                                                                             <  2:288796/143‑1 (MQ=255)
       gCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGACCACGCGACCCg                                                                                                                      >  2:128037/1‑143 (MQ=255)
              ttGTTCATCTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTc                                                                                                         <  1:138896/149‑1 (MQ=255)
                 ttCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTgg                                                                                                      >  1:117896/1‑149 (MQ=255)
                        tAGCGAATGCCGCTGTCAGCACGTTTTTCTGCGCTGTGACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGt                                                                                                     <  2:225943/143‑1 (MQ=255)
                             aaTGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAGTCCAGGAGGCGATTTCCGCCAGAACACCCAGCGACAGCATTGCGGAGGTCACGCGAACCGTACAATCAATTTCTGGTGCcaa                                                                                                >  2:168639/1‑142 (MQ=255)
                             aaTGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCGCGCGAACCGTCCGCTCAATTTCTGGTGCCGCATgggc                                                                                          >  1:31294/1‑148 (MQ=255)
                                           cagGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTaa                                                                            <  1:276168/149‑1 (MQ=255)
                                                   gcTGCGCTGTTACATACATCCCGCGACAAGGCCCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGc                                                                    <  1:106807/148‑1 (MQ=255)
                                                       cgcTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGGGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAACGGttt                                                                      <  2:87555/143‑1 (MQ=255)
                                                        gcTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTCCCGCCAGAACACCCAGCAACAGCATCGCGCAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTg                                                                     <  2:322108/143‑1 (MQ=255)
                                                             taaatacacCCCGCGAGAAGGACCAACAGTCCAGGAGGCGATGTCCGTCCGATCACCCATCAACCGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTg                                                          <  1:70287/146‑1 (MQ=255)
                                                               catacatCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCg                                                        >  1:61396/1‑149 (MQ=255)
                                                                                     aacaaTCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCAt                                        <  2:252202/143‑1 (MQ=255)
                                                                                              agacgCGATTTCCCCCAGAACACCCACCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCa                         <  1:24372/145‑1 (MQ=255)
                                                                                                       ttCCGCCAGAACACCCAGCAACAGCATGGCGGAGATCCCGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCg                      <  2:214674/143‑1 (MQ=255)
                                                                                                               gAACCCCCAGCAACAGCATTGCGGAGATCACGCCAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCGTTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAacca        <  1:144736/149‑1 (MQ=255)
                                                                                                                aaCACCCAGCAACAGCATTGCGGAGAGCCCGCGACCCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAaccac       <  1:6893/149‑1 (MQ=255)
                                                                                                                     ccAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCCGGGCGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACa  >  1:232204/1‑149 (MQ=255)
                                                                                                                                 |                                                                                                                                        
CCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCAGTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACA  >  CP009273/1563732‑1563997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑