| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,215,597 | C→T | 32.2% | intergenic (‑129/‑67) | bglX ← / → dld | beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,215,597 | 0 | C | T | 32.2% | 21.3 / 16.3 | 25 | intergenic (‑129/‑67) | bglX/dld | beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Reads supporting (aligned to +/- strand): ref base C (9/8); new base T (3/5); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
CATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGG > CP009273/2215455‑2215724 | cATAGCCATTTCATATGGATTTTTGGTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGaa > 1:185870/1‑149 (MQ=255) gttgCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTTGCTGAATTGTTATACAAGGCGCTCTTCTAGTTTGTGat > 1:34780/1‑149 (MQ=255) gttgCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTTGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGat > 1:34892/1‑149 (MQ=255) tgCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTtg < 2:4434/143‑1 (MQ=255) tGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGACATAAGCATGATGGAGAGAGAAAAAGCATGCTCAGGTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGcc > 1:269778/1‑149 (MQ=255) gCAGCGAAAATGGTGCAAAAACCGTCGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGccac > 1:206185/1‑149 (MQ=255) cAGCGAAAATTGGGCAAAAACCGTAGGTTGCTATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCCCTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTc < 2:113439/143‑1 (MQ=255) cGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATACTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCtt > 1:9452/1‑149 (MQ=255) aGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTTGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCa < 1:246809/149‑1 (MQ=255) tttGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCCGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACACGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAGAATGTCTTCCGTg > 1:107081/1‑149 (MQ=255) tttGCCATAAGCATGATGGAGAGAGAAAAAGAAGGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCt < 2:290367/143‑1 (MQ=255) tttccATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTTGCTGAATTGTTATCCACGGCGCCGTTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCtt < 2:135015/140‑1 (MQ=255) ttccATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGac < 1:304572/147‑1 (MQ=255) cATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCAt > 2:83823/1‑143 (MQ=255) tAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTTGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGa < 2:262657/143‑1 (MQ=255) tAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGa < 2:241447/143‑1 (MQ=255) cATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGa > 1:283226/1‑149 (MQ=255) agagaAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTTGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCTCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGataat > 2:308683/1‑143 (MQ=255) aGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCtt < 2:219823/143‑1 (MQ=255) tgaGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTtgaat < 2:182430/141‑1 (MQ=255) tCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACtt > 1:247530/1‑149 (MQ=255) tCTGCATTTTCAAAATATTCACTCGCTGACTTGTTATACGAGGCGCTATTCTAGACTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGGCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCCCg > 2:276857/1‑143 (MQ=255) tttCAAAATATTGACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACGGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCtggtg < 2:128839/143‑1 (MQ=255) ttCAAAATATTCACTTGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCtggtgg < 2:30922/143‑1 (MQ=255) ttCAAAATATTCACTTGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCtggtgg < 2:31023/143‑1 (MQ=255) | CATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGG > CP009273/2215455‑2215724 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |