| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 444,779 | C→T | 77.2% | W440* (TGG→TAG) | cyoB ← | cytochrome o ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 444,779 | 0 | C | T | 77.2% | 41.5 / 10.1 | 28 | W440* (TGG→TAG) | cyoB | cytochrome o ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base T (11/10); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 | |||||||||||
TCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCC > CP009273/444635‑444914 | tCAATCTGCTGCCGCAAACGACGGGTCATGCCCATGAAGCACCGCGCATACCGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGcc < 1:378150/145‑1 (MQ=255) cAATGTGCTGGCTCAAACGCCGGGTCATGCCCATGGAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAATCAGAACGCGCGTTGACCCCTGGTTTCGTTCAGTTTGATACCGAACGCTTTAGGcca < 1:272704/145‑1 (MQ=255) tgctgcCTCAAACGACGGGTCATGCCCATGAAGTCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGt < 1:182864/145‑1 (MQ=255) tgctgGCTCAAACGCGGGGTCATCCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGt < 2:106807/145‑1 (MQ=255) ggtcttcgccACGAAGCCCAGGGCATGCAGTGGCAGAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAag < 1:335880/137‑1 (MQ=255) cATGCCCATGAAGCCCAGCGCATACAGTTGCATAAAGGCAACAAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAagcag < 2:32911/145‑1 (MQ=255) aTGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGa < 2:296959/145‑1 (MQ=255) aaGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCa > 2:304713/1‑145 (MQ=255) aaGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGAACa < 2:190817/145‑1 (MQ=255) cccAGCGCATACAGTGGCATAAAGCCGACGAAGAAGCCGATGATCCAGAACCAAAACGCGCGTGTACCCCAGGGATCGTTCAGTCTGAAAGCGAGCGCGTTAGGCTGCTAGTAGGTCATCCCAGCGAAGCAGCCGAGAACAAcgc > 1:325635/1‑145 (MQ=255) aTACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCgatg > 1:156835/1‑145 (MQ=255) tACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGGTCAGTTTGAAACCGCACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCgatga > 2:49944/1‑145 (MQ=255) cAGTGGCAGAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGACCGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATc < 2:218495/145‑1 (MQ=255) cATAAGGGCGACGAAGAAGCCGATGATCCAGGACCAGGACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTa < 1:182475/145‑1 (MQ=255) aaaGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATgg > 1:102905/1‑145 (MQ=255) cAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCAGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATggccggg > 1:146479/1‑140 (MQ=255) aCGAAGTAGCCTCTGTCCCCGAACCATACCGCNCGTTCACCCGAGGTTTCTTTCAGTTTAAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCNGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTgcg < 1:271982/145‑1 (MQ=255) cGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTgcgc > 2:391820/1‑145 (MQ=255) tgatCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAg < 2:344259/145‑1 (MQ=255) tCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCt < 2:102905/145‑1 (MQ=255) ccagaaccagaaCGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTg > 2:421454/1‑145 (MQ=255) cagaaccagaacGCGCGTTCACCCCAGGTTGCGACCAGTTTGAAACCGAACGCTTTAGGCAACCAGTAGGACATCCCTGCGCAGCGGCCGGAGACCGGGCCGCCAATGTGCAGGTTATGAAAGAGCGCATCCAGGAGCAGACTGt > 1:80059/1‑145 (MQ=255) gaaccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTa > 2:155823/1‑145 (MQ=255) cagcacGCGGGTTTACCCCAGGTTTCGGTCAGTTTGAAACCGAACGCTGTAGGCCACCAGTAGGGCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGAGGAGCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCa < 1:407402/145‑1 (MQ=255) gcgcgTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCTATCAGGAACAGGCTGTTATGCAGAACGa < 2:254745/145‑1 (MQ=255) gTTGCGGTCAGTTTGAAACCGAACGCTTTAGGCTACCAGCAGGTCATCCCTGCGCGGCGGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTa < 2:203428/145‑1 (MQ=255) gTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAACAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGGGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGACCGGTAAGGca > 1:82426/1‑144 (MQ=255) aGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTCCGCAATCAGGAACACACTGTTATGCAGAACGAAGTCCGCCCCCGGTACGGCcagca > 1:232987/1‑145 (MQ=255) aCGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTCTGGAAGCGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCAt > 2:236661/1‑145 (MQ=255) gCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATcc > 2:64338/1‑145 (MQ=255) | TCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCC > CP009273/444635‑444914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |