| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,305,991 | G→T | 57.2% | Q80K (CAG→AAG) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,305,991 | 0 | G | T | 57.2% | 6.2 / 18.6 | 21 | Q80K (CAG→AAG) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (3/6); new base T (5/7); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTA > CP009273/2305854‑2306134 | ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTcc < 2:73304/145‑1 (MQ=255) atgTTACGGCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCCCATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGcc < 1:101727/143‑1 (MQ=255) aCGTCATACACAACGCCGTAGCTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACGCGGGTCCAGGAGTCGTTTTCGTTTTCAGCGCTGTTGCTCTGGATCTTATATTCCCACTGGCCGTAAc < 2:259211/145‑1 (MQ=255) cGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAAcc > 1:117060/1‑145 (MQ=255) gTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCg < 2:168500/145‑1 (MQ=255) aacaacGCTGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGc < 2:5313/145‑1 (MQ=255) aacaacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGGTCTGATATTCCCACTGGCCGTAACCGGTCAGc > 1:252884/1‑145 (MQ=255) ccGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGttt < 2:170385/145‑1 (MQ=255) tCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCtt > 1:419959/1‑145 (MQ=255) aaGAACCTACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGaa < 2:410389/145‑1 (MQ=255) aaGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGaa < 2:227474/145‑1 (MQ=255) ttCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCt > 2:261963/1‑145 (MQ=255) tGCGAATGCCACACGGTTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGGCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTGGCCa < 1:216735/145‑1 (MQ=255) gTCCAGGAGTTGTTTTCGTTTTCAGCACTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCCTGTAGGGCTGGTCGCCCTGTACATCCTtgttgt > 1:71722/1‑145 (MQ=38) taGGAGTTGTTTTTGTTTTCAACCCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCag < 2:77078/144‑1 (MQ=255) cacgaggtctttgcgtgttcAGCGCTGTTGCCCTGGATCTTATCTTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCag < 1:313506/136‑1 (MQ=255) gtttgtgtcgttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGaaa < 2:324828/138‑1 (MQ=255) ttgttTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAAt > 1:391667/1‑145 (MQ=255) ttgttTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAAt > 2:280623/1‑145 (MQ=255) tcgcTGGTGCCCTGTATGTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATTTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTa < 1:234368/144‑1 (MQ=255) tGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGGTCTa > 1:274655/1‑145 (MQ=255) | GGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTA > CP009273/2305854‑2306134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |