| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,574,250 | G→A | 18.2% | G419D (GGC→GAC) | tktB → | transketolase 2, thiamine triphosphate‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,574,250 | 0 | G | A | 18.2% | 39.4 / 2.4 | 22 | G419D (GGC→GAC) | tktB | transketolase 2, thiamine triphosphate‑binding |
| Reads supporting (aligned to +/- strand): ref base G (9/9); new base A (2/2); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
TTGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAG‑GTTCTGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTTATACCCACGACTCAATTGGCCTGG > CP009273/2574114‑2574390 | ttGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAG‑GTTCTGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTACGGGGGGCGTGAATTTGGCATGACCGCTCTCGCCAGCGGCATcgctc > 2:151658/1‑143 (MQ=255) tttgttcggATCTTGCTCCCATCCACCTGACCATCTCGATAGAGATAT‑ATTCGCTGACGGAAGATCCAGCGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCAcggcgg < 2:80835/139‑1 (MQ=255) gTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAG‑GTTCTGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCACCGCGCACACcggcgg > 1:76486/1‑145 (MQ=255) gATCTGGCTCCCAGCAACCTGACCATCTAGAAAG‑GTTCTGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCCCTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGc < 1:237131/145‑1 (MQ=255) cTGGCTCCCAGCAACCTGACCATCTGGAAAG‑GTTCTGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACAGCTATCGCACACGGCATCGCGTACCACTGCGGTTTTGTGCCGt < 1:354945/145‑1 (MQ=255) aGCAATCTGATCATTTGGAAAT‑GTTATGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGa < 2:131342/145‑1 (MQ=255) aCCTGACCATCTGGAAAG‑GTTCTGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTCCGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGACATCGCGCACCACGGCGGCTTTGTGCCGAATACCGCGACGtt > 2:60046/1‑145 (MQ=255) aCCTGACCATCTGGAAAG‑GTTCTGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGACATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGtt > 2:268964/1‑145 (MQ=255) tttCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGGTCCTGATGGTTGTTGAATACGACCg > 1:352150/1‑145 (MQ=255) cGCTGAAGGAAGATCCAGCGGGCAACTCTATTCACTACGGGGTGCGTGCACTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACTTTCCTGATGTTTGTTGAATACGCCCGTaa < 1:233154/145‑1 (MQ=255) aTCCAGCGGGCAACTACAATCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGAt > 2:311603/1‑145 (MQ=255) cGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATa < 1:17404/118‑1 (MQ=255) cGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATa > 2:17404/1‑118 (MQ=255) gtgtgtgtGAATTTGGCATGACCGCTATGGCCAACGGCCTCGCGCACCACGGCGGCTTTGTGCCGTATCCCGCGACGTTTCTGACGTTTGTTGCATCCGCCCGTGACACCGCGCGGCTGCCGCCATTGTGGAACGCGCCGCAGAt > 1:40287/3‑145 (MQ=255) gCGTGGATTTTGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCGGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATg < 1:60180/145‑1 (MQ=255) tttGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTtata > 1:338757/1‑145 (MQ=255) cATGCCTGCTATCGGCAACGGCATCGCGCACCACTGCGGCTTTGTGCCGTATACCGCGACGTTCCTGCTGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTTATACCCAc < 2:239985/145‑1 (MQ=255) aTGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTTATACCCACg > 2:338715/1‑145 (MQ=255) aTGACCGCTATCGCCAACGACCTCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTTATACCCACg < 1:60046/145‑1 (MQ=255) aTGACCGCTATCGCCAACGACATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTTATACCCACg < 1:268964/145‑1 (MQ=255) tcAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTTATACCCACGACTCAATTGGCCt > 2:138065/2‑145 (MQ=255) aaCGGCATCGCGCACCACGGCGGCTTGGTGCCGTATACCGCGACGTCCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTTATACCCACGACTCAAGTGGCCTgg < 1:336578/145‑1 (MQ=255) | TTGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAG‑GTTCTGTTTCGCTGAAGGAAGATCCAGCGGGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGTGCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGCAGATTATGGTTTATACCCACGACTCAATTGGCCTGG > CP009273/2574114‑2574390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |