Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,701,955 G→A 4.4% intergenic (‑890/+21) dppA ← / ← proK dipeptide transporter/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,701,9550GA 4.4% 294.9 / 2.7 100intergenic (‑890/+21)dppA/proKdipeptide transporter/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (47/47);  new base A (2/3);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-01

Only 100 of 103 total aligned reads displayed.

TCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCT  >  CP009273/3701811‑3702098
                                                                                                                                                |                                                                                                                                               
tCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGGGGTGCAGATGCGAACAAGCCTGGTCg                                                                                                                                                 >  1:187069/1‑145 (MQ=37)
tCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCACATGCAAAAAAGCCTGCTCg                                                                                                                                                 >  2:580/1‑145 (MQ=255)
tCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGCCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCGAAAAAGCCTGCTCg                                                                                                                                                 >  1:383817/1‑145 (MQ=255)
  ggTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGtt                                                                                                                                               >  2:150217/1‑145 (MQ=255)
       tAGAGTATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCa                                                                                                                                          <  2:326268/145‑1 (MQ=255)
           ggATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCt                                                                                                                                      >  1:397209/1‑145 (MQ=255)
            gtttACTCGCCACTTCGTGGTTCGCCCTGCGGGCCGTTGCTGGCCCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGCGATGCAGAGGCAAAAAAGCCTGCTCAACGAGCAGGCtt                                                                                                                                     <  1:236044/143‑1 (MQ=25)
            gATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCtt                                                                                                                                     >  2:188546/1‑145 (MQ=255)
            gATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtt                                                                                                                                     <  1:235994/145‑1 (MQ=255)
            gATTACTCGCCACTTCGTGGCCCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTTGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAACTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCtt                                                                                                                                     <  2:273766/145‑1 (MQ=255)
              ttACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAACAAAGCCTGCTCGTTGAGCAGGCcttt                                                                                                                                   >  2:31981/1‑145 (MQ=255)
               tccTCGCCACTTGGTGGCTCGCCCTGCGGGTCGTTGTTGGGGCATCGTTCTCTTGCTTTGCTCGAGTCGAACCTCTCTGCCCCCGGAGGTTGTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCttttt                                                                                                                                  <  2:24733/143‑2 (MQ=18)
                 cgagccacgaagTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCCGAGGTTCTCATCCTCATTAGCTGCAGATGCAAAAAAGCCTGATCGTTGAGCAGGCTTTTCGa                                                                                                                                >  2:152067/12‑145 (MQ=21)
                 cTCGCCACTTCGTGGCTCGCCTTGCTGGCCGTTGCTGGCGCAACGTTCTCTCGCGTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGa                                                                                                                                <  2:373718/145‑1 (MQ=255)
                  tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                                                               >  1:409409/1‑145 (MQ=255)
                  tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                                                               >  1:356458/1‑145 (MQ=255)
                  tCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGGTCCCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                                                               >  1:400805/1‑145 (MQ=255)
                  tCGCCACTGCGTGGCGCGCCCGGCGGGCCGTTGGTGGCGCAACGTTCTCTCGCCTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                                                               <  2:108818/145‑1 (MQ=18)
                    gCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAtt                                                                                                                             >  1:15744/1‑145 (MQ=255)
                        cTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATACCAAAAAACCTGCCCGCTGAGCAGGGTTTTCGAAATTGGt                                                                                                                         >  1:336672/1‑145 (MQ=21)
                         ttCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTc                                                                                                                        >  1:414554/1‑145 (MQ=255)
                           cgcggcgcccccTGCGGACCGTTGCCGGCCCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCgg                                                                                                                      <  2:13412/136‑1 (MQ=17)
                            gcggttccgccTGCGGGTAGGTGCCGGCGCCGCGTTCTCTGGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGt                                                                                                                     <  1:34379/136‑1 (MQ=14)
                              ggctcgtccgtaggggCGGTGCCGGCGCAACGTTCTCACGCATCGCTCGCGTCGAACCTCCCTGCCCCCGGAGTTTCTCATCCTCACGTGCTGCAGATGCACCGTAGCTTTCTGGTTTAGCAGGCTTTTCGAATTTGGTCGGTGa                                                                                                                   <  1:177524/133‑1 (MQ=9)
                                  cGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATaga                                                                                                               <  1:47799/145‑1 (MQ=255)
                                   gCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGGTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGCGCAGGCTTTTCGAATTTGGTCGGTGATagag                                                                                                              >  2:279732/1‑145 (MQ=255)
                                   gCCCGGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATagag                                                                                                              <  2:225605/145‑1 (MQ=255)
                                     ccgacGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGa                                                                                                            <  2:378227/141‑1 (MQ=255)
                                      cTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATAAGCTGCAGATGCAAAAATGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAt                                                                                                           >  1:266790/1‑145 (MQ=255)
                                       tGCGGGCCGTTGCTGGCGTAATGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAtt                                                                                                          <  2:33191/145‑1 (MQ=255)
                                       tGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAtt                                                                                                          >  2:163549/1‑145 (MQ=255)
                                       tGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAtt                                                                                                          >  1:365109/1‑145 (MQ=255)
                                               tttGCTGCGCTACGTTCTCTCGCTTAGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGACTTTGGTCGGTGATAGAGGATTCGAACCTcc                                                                                                 <  1:395394/144‑1 (MQ=17)
                                               gTTCCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTc                                                                                                  <  2:292474/145‑1 (MQ=255)
                                                  gCTGGCGCTACGTTCTCTAGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGa                                                                                               <  2:85943/145‑1 (MQ=255)
                                                    tGGCCCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGAcc                                                                                             >  1:368704/1‑145 (MQ=255)
                                                     ggCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGCTGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACATCCGAccc                                                                                            >  1:13115/1‑145 (MQ=255)
                                                      gcgcAACGTTCTCTCGCCTCGCTGGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGAcccc                                                                                           <  1:74043/145‑1 (MQ=255)
                                                       cgcAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCt                                                                                          >  2:89083/1‑145 (MQ=255)
                                                        gcAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAACCCTGCTCATTGAACAGGCTTTTCGAATTTGGTCGGTGATAGAGGGTTCGAACCTCCGCCCCCtt                                                                                         >  2:3259/1‑145 (MQ=18)
                                                        gcAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCtt                                                                                         >  2:67636/1‑145 (MQ=255)
                                                        gcAACGTTCTCTCGCTTCGCTCGACTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGTAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCAGACCCCtt                                                                                         >  2:316487/1‑145 (MQ=255)
                                                         cAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTc                                                                                        >  2:400054/1‑145 (MQ=255)
                                                          aaCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGCGTCGAACCTCCGCCCCCTTCg                                                                                       >  2:325814/1‑145 (MQ=255)
                                                          aaCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTCTCGAATTTGGCCGGTGATAGAGGATTCGAACCTCCGAccccaccg                                                                                       >  2:342179/1‑141 (MQ=255)
                                                             gTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGCGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTcc                                                                                    <  2:62106/145‑1 (MQ=255)
                                                                ctctgGTTTGGCGCGAGTCGAACCTCCTGGCCCCCGGAGGTTCTCATCCGCATGGGCTGCAGATGCAAAAAAGCCTGCGCTTTGTGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCcga                                                                                 <  1:113889/145‑1 (MQ=17)
                                                                ctctcGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCATTCGTCCcga                                                                                 >  1:171105/1‑145 (MQ=255)
                                                                ctcgcGCCTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCcga                                                                                 <  1:337398/145‑1 (MQ=255)
                                                                  ctcGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTAATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCcgaac                                                                               <  2:127044/145‑1 (MQ=255)
                                                                  cccggttccctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCcgaac                                                                               <  1:207155/140‑1 (MQ=255)
                                                                     gcttcgctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCcgaacgaa                                                                            <  2:401354/145‑1 (MQ=255)
                                                                         cgctCTAGTCGAACTTTCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTgc                                                                        <  2:18941/145‑1 (MQ=255)
                                                                           ctCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTgcgc                                                                      <  1:261016/145‑1 (MQ=255)
                                                                            tCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTgagcg                                                                     >  2:353359/1‑144 (MQ=255)
                                                                                   gaagttCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGTAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGGGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGc                                                              <  2:187086/140‑1 (MQ=255)
                                                                                   gAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGGCCCCTTCGTCCCGAACGAAGTGCGCTTCCAGGc                                                              >  2:326144/1‑145 (MQ=255)
                                                                                     tccTCCGTGCCCCCGGAGGTTCTCATCCTCATGGTCTGCAGATCCAAAAAATCCTTCTCGGTGATCACGCTCTTCGAATTTGGTCGGTGATAGAGTATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTg                                                            <  1:87087/144‑1 (MQ=21)
                                                                                     aCCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTg                                                            <  1:94400/145‑1 (MQ=255)
                                                                                       cccccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcg                                                          <  2:251186/143‑1 (MQ=255)
                                                                                        tCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCtactc                                                         >  2:54148/1‑141 (MQ=255)
                                                                                        tCCCTGCCCCCGGAGGTTCTCATCCACATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgc                                                         >  2:252727/1‑145 (MQ=255)
                                                                                            tGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGAAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTCGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAAt                                                     <  2:128412/145‑1 (MQ=255)
                                                                                              cccccGGAGGCTCTCATCCTCATGCGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGGCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCa                                                   >  1:99484/1‑145 (MQ=255)
                                                                                               ccccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAc                                                  <  2:62489/145‑1 (MQ=255)
                                                                                               ccccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCCTACCCCCGTCCCGTCCCGCCCGACCGGACGGCGCTAGCGGCCCGCGCCAACCAc                                                  >  1:65528/1‑145 (MQ=11)
                                                                                               ccccGGAGGTTCTCATCCTCATGAGCTACAGATGCAAAAAAGCCTGATCGTTGAGCAGTCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGAGCCAACCAc                                                  >  1:1683/1‑145 (MQ=21)
                                                                                                       gatctcATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCg                                          >  2:272534/3‑145 (MQ=255)
                                                                                                        ttCTCATCCTCATAAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGGAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGTGCTGCCAGGCTGCGGCAATCACCGAATGCgg                                         >  1:258518/1‑145 (MQ=25)
                                                                                                         tctcATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCggg                                        >  1:416263/1‑145 (MQ=255)
                                                                                                           tcATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACAGGCTGCGCCAATCACCGAATGCGGGgcg                                     >  2:423280/1‑145 (MQ=255)
                                                                                                             taccTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgcgc                                    <  1:308435/143‑1 (MQ=255)
                                                                                                               ccTCATGAGCTGCAGATGCAAAAAAGCCTTCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGTGCCAATCACCGAATGCGGGGCGCAt                                  <  2:253698/145‑1 (MQ=255)
                                                                                                                 tCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCt                                >  1:199055/1‑145 (MQ=255)
                                                                                                                 tCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCt                                >  2:339509/1‑145 (MQ=255)
                                                                                                                 tCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCt                                >  1:284290/1‑145 (MQ=255)
                                                                                                                     gAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGATTTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACt                            <  2:148361/145‑1 (MQ=255)
                                                                                                                         tGCAGATGCAAAAATGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcgc                        <  1:65925/145‑1 (MQ=255)
                                                                                                                         tGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGGATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcgc                        <  2:44568/145‑1 (MQ=255)
                                                                                                                         tGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCCCCGAATGCGGGGCGCATCTTACTgcgc                        >  1:410176/1‑145 (MQ=255)
                                                                                                                         ggCAGATGCAAAACAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcgc                        <  1:253198/144‑1 (MQ=255)
                                                                                                                          gCAGATGCAAAAAAGCCTGCTCGTTGAGCCGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCa                       <  1:186926/145‑1 (MQ=255)
                                                                                                                          gCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCa                       <  1:150217/145‑1 (MQ=255)
                                                                                                                          gCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTACGCa                       >  2:81889/1‑145 (MQ=255)
                                                                                                                           cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGCATGCGGGGCGCAGCTTCATGCGCac                      >  1:99536/1‑144 (MQ=255)
                                                                                                                             gacgCAAAAACGCCTGCTCGTTGAGCAGCCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGAt                    <  2:178072/142‑1 (MQ=255)
                                                                                                                             gATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGTCTACAGCCAAGAATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGAt                    <  2:138413/145‑1 (MQ=38)
                                                                                                                              aTGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATa                   <  1:188546/145‑1 (MQ=255)
                                                                                                                                gCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACg                 >  1:124019/1‑145 (MQ=255)
                                                                                                                                 cAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGc                <  2:65528/145‑1 (MQ=255)
                                                                                                                                    aaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCt             <  2:232725/145‑1 (MQ=255)
                                                                                                                                      aaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGAGGCGCATCTTACTGAGCAGATACGCCCTCg           >  2:149667/1‑145 (MQ=39)
                                                                                                                                       aGCCTGCGCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGt          <  1:246066/145‑1 (MQ=255)
                                                                                                                                          cTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCCGATACGCCCTCGTCaa       <  2:266790/145‑1 (MQ=255)
                                                                                                                                           tGCTCGTTGAGCAGGCATCTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCAGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCGTCTTACTGCGCAAATACGCCCTCGTCAAt      <  2:267807/145‑1 (MQ=39)
                                                                                                                                            gCTCGTTGAGCAGACTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTACCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATc     >  2:290066/1‑145 (MQ=255)
                                                                                                                                             cTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATCGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGCGCGCATCTTACTGCGCCGATACCCCCTCCTCAATcc    >  1:76065/1‑145 (MQ=39)
                                                                                                                                             cTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATcc    <  1:339081/145‑1 (MQ=255)
                                                                                                                                             cTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATcc    >  1:159177/1‑145 (MQ=255)
                                                                                                                                               cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGAGCCATTCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCt  <  2:330804/145‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCT  >  CP009273/3701811‑3702098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: