| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,772,673 | T→A | 12.1% | L87Q (CTA→CAA) | lldR → | dual role activator/repressor for lldPRD operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,772,673 | 0 | T | A | 12.1% | 74.8 / 2.4 | 33 | L87Q (CTA→CAA) | lldR | dual role activator/repressor for lldPRD operon |
| Reads supporting (aligned to +/- strand): ref base T (14/15); new base A (2/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
CAACTCGGCGTATCACGTAATTCACTGCGCGAGGCGCTGGCAAAACTGGTGAGTGAAGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATTCAGCTTTGCTTTGAAGCAAC > CP009273/3772531‑3772817 | cAACTCGGCGTATCACGTAATTCACTGCGCGAGGCGCTGGCAAAACTGGTGAGTGAGGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGATTATTCGCTGGCGTCATGACAAGTGGTCGGAGCAAAACATCGTCCAGCCGCTaa > 2:235651/1‑145 (MQ=255) tagcACGTAATTCACTGCGCGAGGCGCTGGCAAAACTGGTGAGTGAAGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGAt < 2:199427/142‑1 (MQ=255) tATCACGTAATTCACTGCGCGAGGCGCTGGCAAAACTGGTGAGTGAAGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGAt > 2:228787/1‑145 (MQ=255) aCTGCGCGAGGCGCTGGCAAAACTGGTGAGTGAAGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCg < 1:227812/145‑1 (MQ=255) aCTGCGCGAGGCGCTGGCAAAACTGGTGAGTGAAGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCAAAAAACACTGATGGCCGATGATCCg > 2:123991/1‑145 (MQ=255) aCTGCGCGAGGCGCTGGCAAAACTGGTGAGGGAAGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCAAAAAACACTGATGGCCGATGATCCg > 2:332612/1‑145 (MQ=255) cTGCGCGGGGCTCTGGCAAACCTGGTGAGTGCAGGCGTGCTTCTCAGTCGCCGCGGCGGCGGGACGTTTATTCGCTGGCGTCATTACACATGGTCGGAGCAAAATATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCgg < 1:79795/145‑1 (MQ=255) cgcTGGCAAAACTGGTGAGTGAAGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTAATGACACCTGGTCGCACCAAACCACCGAACAGACGCTAAAATTATTGATGGCCGATGATCCGGTTTATAGttt < 1:101831/145‑1 (MQ=255) gttgggtgaAGGCGTGCTGCTCCGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGaa < 2:386791/140‑1 (MQ=255) ggCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACg < 2:401559/145‑1 (MQ=255) ctgctCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATTATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTg < 2:376728/145‑1 (MQ=255) ctgcctaGTGGACGCGGCGGTGGGACGTTTATTCGCTGGTGTCTTGACACATGGACGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTg < 2:17396/139‑1 (MQ=255) tCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGc > 2:383930/1‑145 (MQ=255) tCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGGCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGAGCCGGATTACCGTTTCGATATCCTGGAAGGCCGCGACGCCATTGGAGCCAGc > 2:178749/1‑145 (MQ=255) gcggcggcgGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCgc > 2:104157/1‑145 (MQ=255) aCGTTTATTCGCTGTGGTGATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCgg < 2:120344/145‑1 (MQ=255) aCGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCgg < 2:384465/145‑1 (MQ=255) gTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCa > 1:103039/1‑145 (MQ=255) ttATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAAt > 2:107029/1‑145 (MQ=255) aTGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACAGCTgg > 1:214575/1‑145 (MQ=255) acacGTGGTGGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGCAGCCCGCTACGCCATTGAAGCCAGCACCGCATGTCATGCGGCAATGCGCGCCACCCCTGGCGa > 1:216781/1‑145 (MQ=255) cacaTGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGAc < 1:228787/145‑1 (MQ=255) cacaTGGTCGGAGCAAAACATCGTCCAGCCGCAAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGAc < 1:332612/145‑1 (MQ=255) cacaTGGTCGGAGCAAAACATCGCCCAGCCGCAAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGAc < 1:123991/145‑1 (MQ=255) tCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACaaagaaa > 1:232244/1‑145 (MQ=255) tCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGAGTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACaaagaca > 1:105110/1‑143 (MQ=255) tCGGAGCAAAACATCCTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACaaagcaa > 1:366393/1‑145 (MQ=255) cATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCAGTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATTCAgctt < 2:256412/145‑1 (MQ=255) gTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATTCAgctttgct > 1:52879/1‑145 (MQ=255) gCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCCCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATTCAGCTTTGCTTTGaa < 2:304642/145‑1 (MQ=255) ccGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATTCAGCTTTGCTTTGAAg > 2:202004/1‑145 (MQ=255) gCTAAAACCACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATTCAGCTTTGCTTTGAAGCa < 1:182792/145‑1 (MQ=255) cTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATGCAGCTTTGCTTTGAAGCaa < 2:307276/145‑1 (MQ=255) tAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATTCAGCTTTGCTTTGAAGCAAc > 2:197695/1‑145 (MQ=255) | CAACTCGGCGTATCACGTAATTCACTGCGCGAGGCGCTGGCAAAACTGGTGAGTGAAGGCGTGCTGCTCAGTCGACGCGGCGGCGGGACGTTTATTCGCTGGCGTCATGACACATGGTCGGAGCAAAACATCGTCCAGCCGCTAAAAACACTGATGGCCGATGATCCGGATTACAGTTTCGATATTCTGGAAGCCCGCTACGCCATTGAAGCCAGCACCGCATGGCATGCGGCAATGCGCGCCACACCTGGCGACAAAGAAAAGATTCAGCTTTGCTTTGAAGCAAC > CP009273/3772531‑3772817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |