Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,207,564 A→T 13.8% intergenic (‑105/+595) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,207,5640AT13.8% 65.2 / 4.9 29intergenic (‑105/+595)iraM/ycgXRpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base A (13/12);  new base T (2/2);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

TAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACA  >  CP009273/1207423‑1207691
                                                                                                                                             |                                                                                                                               
tAGGTTGAATTTCCGTGTCAATTACTATCCACTTCGTTTGTCATACCTGTCCGCCCTTAAGTTGGGTTGGTTCCTTCGGAGCTTTATTTCTCAATTTTGCATATGAGTATATTACCCCCCCCTCAAAACAATAAATTAATTaaaa                                                                                                                              <  1:299162/145‑1 (MQ=255)
tAGCTTAAATTTCCTTGTCAATTACTATCCAATTCATTTTTTATTTCTTATCCCACGTTATTTAATATGGGTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTaaaa                                                                                                                              <  1:57824/145‑1 (MQ=255)
                 tCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATataaaa                                                                                                             >  1:230616/1‑145 (MQ=255)
                   aaTTACTATCGACTTCATTTGTTATGTCTTATCCAAAGGTATTTCCTATGGTTCATTAGGTTGTTTATTTCTTGCGTTTGAATATCAGAATATTACCCCCCCCTCACAAAAATAAATTAATTAAAATTATGGCTTATataaaata                                                                                                           >  1:158367/1‑145 (MQ=255)
                           tCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa                                                                                                   >  2:18415/1‑145 (MQ=255)
                            ccACATCATTTGTTATGTTTCATGCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCACATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaaa                                                                                                  <  2:88814/145‑1 (MQ=255)
                             cACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTTAAATGATGGCTTATATAAAATAAAATTTAAAg                                                                                                 >  1:284542/1‑145 (MQ=255)
                             cACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTTAAATGATGGCTTATATAAAATAAAATTTAAAg                                                                                                 >  1:258608/1‑145 (MQ=255)
                                ttCATTTTTTATGTCTTACACCAGTGTTTTTAATATGGTTCATTAGTATGTTTATTTCTTGATTTTGCATATGATTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCaa                                                                                              <  1:324366/145‑1 (MQ=255)
                                               ttATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGAt                                                                               <  1:423505/145‑1 (MQ=255)
                                               ttATCCCACGCTATTTAATATGGTTCTTTAGTATCTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATCATGGCTTATATAAAATAAAATTTAAAGCAACGAATCTCAATGGAt                                                                               <  1:284963/145‑1 (MQ=255)
                                                 aTCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGt                                                                             >  2:337709/1‑145 (MQ=255)
                                                                 tatGGTTCATTAGGATGCTTATTTCTTGATTTTGCATATGAGTATCTTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGTTTATATAAAATAAAATTTAAAGGAAGGAATCTCAATGGATGTTAAACAAAATGAGAtt                                                             <  2:331682/145‑1 (MQ=255)
                                                                   tGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAtttt                                                           <  2:401844/145‑1 (MQ=255)
                                                                            aGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATGAAATAAAATTTAAAGCAAGGAATCTCAATGGCTGTTAAACAAAATGAGATTTTGTTAAAGCa                                                  >  2:75210/1‑145 (MQ=255)
                                                                                     aTTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAattat                                         <  1:347712/145‑1 (MQ=255)
                                                                                        tCTTGATTTTGCATATGAGTATATTACCACCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAGACAAGTAGTTTAAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAattattta                                      >  2:203403/1‑143 (MQ=255)
                                                                                            gATTTTGTATATGAGGATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTc                                  <  2:94308/145‑1 (MQ=255)
                                                                                            gATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTAAACGCACGGTGTCTCGATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTc                                  >  1:256521/1‑145 (MQ=255)
                                                                                                      atGAGTATCTTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATCGCTTATATAAAATAAAATTTACAGCAAGGAATCTCAATGGATGTTAAACAAAAGGAGATTTTGTGCAAGCAATAAATAATTGACATCGTTTTAGAtt                        >  1:9191/1‑145 (MQ=255)
                                                                                                        gAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAAGCTCAATGGATGTTAAACAAAATGAAATTTTGTGCAAGCAATAAATTCTTGACTTGCTTTTAGATTTg                      >  1:358520/1‑145 (MQ=255)
                                                                                                           tataTTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGttt                   <  2:225985/145‑1 (MQ=255)
                                                                                                             tatTACCCCCCCCTCAAAAAAATAAATTAATTTAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATACATTATTGACTTCGTTTTAGATTTGTTTAg                 <  2:258608/145‑1 (MQ=255)
                                                                                                             tatTACCCCCCCCTCAAAAAAATAAATTAATTTAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAg                 <  2:284542/145‑1 (MQ=255)
                                                                                                                   cccccccTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATaa           >  2:122259/1‑145 (MQ=255)
                                                                                                                     cccccTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAACGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATg         >  2:69038/1‑145 (MQ=255)
                                                                                                                        ccTCAAAAAAATAAATTAATTAAAATGATTGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTta      <  1:359666/145‑1 (MQ=255)
                                                                                                                        ccTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTta      <  2:158367/145‑1 (MQ=255)
                                                                                                                          tCAAAAAAATAAATTTATTAAAATTATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTtata    <  1:125292/145‑1 (MQ=255)
                                                                                                                            aaaaaaaTAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGTTTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTtataaa  >  1:315631/1‑143 (MQ=255)
                                                                                                                            aaaaaaaTAAATTAATTAAAATGATGGATTAAATAAAATAAAAATTAAAGGGTGAAATCTCAATAAAAGTAAAACAAGATGAGAATTTGTGAAGTCAATTAATTATTGACTTAGTTTTAGATTTGTTTAGCTTTAATGTTATACa  >  1:84747/1‑145 (MQ=255)
                                                                                                                                             |                                                                                                                               
TAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACA  >  CP009273/1207423‑1207691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: