Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,371,135 G→T intergenic (‑356/+39) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,371,1350GT98.6% 206.0 / NA 74intergenic (‑356/+39)sspA/rpsIstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (39/33);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAG  >  CP009273/3370989‑3371280
                                                                                                                                                  |                                                                                                                                                 
gaTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGGGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGttt                                                                                                                                                 <  1:731936/149‑1 (MQ=255)
 aTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGtttt                                                                                                                                                <  2:1092608/149‑1 (MQ=255)
  tGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTtttt                                                                                                                                               <  2:385206/149‑1 (MQ=255)
    tgcTTTAGTCTTAAATAGGTGCGTTAGTCCCTTTTTGGCAATGTGGTTTTTGTTTTAAATAAATTTTAGGTGGCAGCAAAAGGCAATTTTCCAGTTTTTACGCTGACTCAGATTTTCGCTATAAAAAAACCCGCCGAAGCGGTtttttt                                                                                                                                             <  1:327540/148‑1 (MQ=255)
     gcgttggTCTGAAATAGTTCACTTCGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCGTTCAGGTGGCAGCAACAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAATCCCGCCGAAGCGGTTTTTTTc                                                                                                                                            <  2:691328/143‑1 (MQ=255)
            ttcgAAATAGTTGACTTAGTCCCTTTTTGGCGATGGGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAATCCCGCCGAAGCGGTTTTTTTCGAAAAtt                                                                                                                                     <  2:141789/146‑1 (MQ=255)
            tCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCCGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGCAGCGGGTTTTTTCGAAAAtt                                                                                                                                     >  1:5036/1‑149 (MQ=255)
              tGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGt                                                                                                                                   >  2:528488/1‑149 (MQ=255)
              tGAAATAGTTGACTTAGTCCCTTAGTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAAttat                                                                                                                                   >  1:1037151/1‑147 (MQ=255)
               gAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAATGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGtt                                                                                                                                  >  2:236149/1‑149 (MQ=255)
                 aaTAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGtttt                                                                                                                                >  2:814065/1‑149 (MQ=255)
                     gTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGc                                                                                                                            <  1:967723/149‑1 (MQ=255)
                      ttGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTGTTTTTCGAGAATTGTTTTCTGcc                                                                                                                           >  2:911597/1‑149 (MQ=255)
                       tGACTTAGTCCCTTATGGGCGATGTTGTTTTTGTTCTACCTGTCTGGCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCg                                                                                                                          <  1:49927/149‑1 (MQ=255)
                         aCTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGAAAAAAGCAACTATCCAGTTTTTACGCTGATTCAGAGTTTAGCTCTAAAAAAACCCGCCGAAGCGGTGTTTTTCGAAAATTGTTTTCTGCGGGa                                                                                                                        >  2:533741/1‑149 (MQ=255)
                         aCTTAGTCCCTTATTGGCGATGTGGTGTTTGTTTTACCTGTCGGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCATATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTTCCGGa                                                                                                                        <  2:979469/149‑1 (MQ=255)
                             aGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAg                                                                                                                    >  1:924477/1‑149 (MQ=255)
                               tCCCTTATTGGCGATGTGGTTTTTGTTTTACCGGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGaa                                                                                                                  <  1:765164/149‑1 (MQ=255)
                                tccTTATTGGCGATGTGGTTTTTGTCTTACCTGTCTGTCAGGATGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGTCGTAGTGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAg                                                                                                                 <  2:697410/148‑1 (MQ=255)
                                 ccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCTGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGc                                                                                                                >  1:934545/1‑149 (MQ=255)
                                 ccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGc                                                                                                                >  1:1239836/1‑149 (MQ=255)
                                  cTTATTGGCGATGTTGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTTGCTATAAAAAAGCCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc                                                                                                               <  2:721438/149‑1 (MQ=255)
                                       tGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTATTTTTCGAAAATTCTTTTCTCCCGGAGCAGAACCCAATTa                                                                                                          >  2:663435/1‑149 (MQ=255)
                                        ggCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAAATTTCCAGTTTTTACGCTGATTCAGATTTTGGCTATAAAAAAACCCGCCGAATCGGTTTTTTGCCATAATTATTTTATGCAGGGGCAgtgactaatatg                                                                                                         >  1:637642/1‑138 (MQ=255)
                                          cGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACg                                                                                                       >  2:809355/1‑149 (MQ=255)
                                            aTGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGtt                                                                                                     >  1:59684/1‑149 (MQ=255)
                                              gtgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg                                                                                                   >  2:661806/1‑149 (MQ=255)
                                               tgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCCAAACTTGTTTTCTGTCGGATCTGAAGCCCATTAACGTTTgg                                                                                                  >  1:73977/1‑149 (MQ=255)
                                                   ttttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgagag                                                                                              >  1:252207/1‑148 (MQ=255)
                                                     ttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACt                                                                                            <  1:1288120/149‑1 (MQ=255)
                                                        ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCACCTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGGGCAGAAGCCAATTAACGTTTGGAGAACcgcg                                                                                         >  2:1149730/1‑149 (MQ=255)
                                                        ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCg                                                                                         >  1:1149791/1‑149 (MQ=255)
                                                          ttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGga                                                                                       <  1:932073/149‑1 (MQ=255)
                                                           tACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgac                                                                                      <  1:540868/149‑1 (MQ=255)
                                                            aCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGACGCGGTTTTTTTCGAAAATTGTTTTCAGCCGGAGCAAAAGCCAATTAACCACTAGAGAACGGCGcacg                                                                                     >  2:275117/1‑149 (MQ=255)
                                                              ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTATAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTACCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgac                                                                                   >  2:1022487/1‑149 (MQ=255)
                                                                gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGTTTACTTTTGTATCTATAAAAAAACCCGCCGAAGCGGTTGTTTTCGAAAATTGTTTTCCGCCGGAGCAGAAGCCAATCAACGTTTGGAGAACTGCGgacgacgc                                                                                 >  2:920241/1‑148 (MQ=255)
                                                                gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGCAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacga                                                                                 <  1:643148/149‑1 (MQ=255)
                                                                    gtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGAGGACGTg                                                                             >  1:997125/1‑149 (MQ=255)
                                                                     tgaGGTGGCAGCAAAAAGCAACTTGCCAGTTTGTACGCTGATTGAGATGTTGGTGATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGc                                                                            <  2:1195340/147‑1 (MQ=255)
                                                                     tcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGc                                                                            <  1:762884/149‑1 (MQ=255)
                                                                      cAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGGTTGGAGAACTGCGGACGCCGACGTGCt                                                                           >  1:156295/1‑149 (MQ=255)
                                                                         gTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGGGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTa                                                                        >  1:532482/1‑149 (MQ=255)
                                                                          tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGCACAGTAATTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAGCTGCGGACGACGACGTGCTTTAc                                                                       >  1:328082/1‑149 (MQ=255)
                                                                           ggCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACg                                                                      <  2:904196/149‑1 (MQ=255)
                                                                              agcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAg                                                                   >  2:454934/1‑149 (MQ=255)
                                                                               gcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGa                                                                  >  2:765572/1‑149 (MQ=255)
                                                                                 aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGGAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTCACGTTTGGAGCACTGCGGACGACGACGGGCTTTACGcgcacc                                                                >  1:1350337/1‑144 (MQ=255)
                                                                                   aaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGa                                                              >  2:1265220/1‑149 (MQ=255)
                                                                                      gCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACtt                                                           <  2:147467/149‑1 (MQ=255)
                                                                                       cAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACttt                                                          <  1:1312942/149‑1 (MQ=255)
                                                                                        aaCTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTc                                                         >  1:474412/1‑149 (MQ=255)
                                                                                          cTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTCACGTTTGTAGAACTGCGGACGACGACGGGCTTTACGCAGACCGACTTTCtt                                                       >  1:608196/1‑149 (MQ=255)
                                                                                           tttCCAGTTTTTACGCTGATTCGGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCGGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTa                                                      <  2:2249/149‑1 (MQ=255)
                                                                                           tttCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTa                                                      <  1:740891/149‑1 (MQ=255)
                                                                                            ttCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTAc                                                     <  1:570620/149‑1 (MQ=255)
                                                                                               cattttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGtt                                                  <  2:896584/146‑1 (MQ=255)
                                                                                                aGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGtta                                                 >  1:166093/1‑148 (MQ=255)
                                                                                                  tttttACGCTGATTCAGATTTTATCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCaa                                               <  1:661806/149‑1 (MQ=255)
                                                                                                  ttttcACGCTGTTCCAGATTTTAGCTATAAAAAAACCCGCAGAAGCGGTTGTTTTCGACAGTTGTTTTCTGCCGGAGCAGAATCCAATTAACGTTTGGAGCACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCaa                                               <  2:680200/149‑1 (MQ=255)
                                                                                                          cTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGa                                       <  1:444842/149‑1 (MQ=255)
                                                                                                           tGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGCCGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAg                                      >  2:47005/1‑149 (MQ=255)
                                                                                                            gATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGc                                     >  2:1102047/1‑149 (MQ=255)
                                                                                                               tCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGAATTTTTGTAAAATTGTTTTCTGCCGAAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGAGTTTCTTACGTTCAACCTGACGAGCGTc                                  >  1:335959/1‑149 (MQ=255)
                                                                                                                cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGCCa                                 >  2:917701/1‑149 (MQ=255)
                                                                                                                 aGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTGTTTTCCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                                <  2:73977/149‑1 (MQ=255)
                                                                                                                      ttAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTa                           <  2:632023/149‑1 (MQ=255)
                                                                                                                           tataAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGaa                      <  2:864443/149‑1 (MQ=255)
                                                                                                                            ataAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAg                     >  2:406014/1‑149 (MQ=255)
                                                                                                                            ataAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGATTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAg                     >  1:1192476/1‑149 (MQ=255)
                                                                                                                             taAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGGAACGAAGc                    >  1:166781/1‑149 (MQ=255)
                                                                                                                                  aaaCCCGCCGAAGCCGTTTTTTTCGAAAATTGTTCTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCt               <  1:377132/149‑1 (MQ=255)
                                                                                                                                      ccgccgAAGCGGTTTTTTTCGAAAATTGTCTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTAc           >  1:666820/1‑149 (MQ=255)
                                                                                                                                       cgccgAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACg          <  1:1210954/149‑1 (MQ=255)
                                                                                                                                          cgAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAg       <  1:454934/149‑1 (MQ=255)
                                                                                                                                               cGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACCGAGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGCGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAg  <  1:809355/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAG  >  CP009273/3370989‑3371280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: