Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,138,810 G→T D338E (GAC→GAA)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,138,8100GT93.8% 257.1 / ‑4.2 96D338E (GAC→GAA) rnefused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (46/44);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATGG  >  CP009273/1138662‑1138955
                                                                                                                                                    |                                                                                                                                                 
aCATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAgg                                                                                                                                                   >  1:31548/1‑149 (MQ=255)
  acttCCAGCAGGCCAAAGCGAGAAATATGGCTTATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGGTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTc                                                                                                                                                 <  1:790795/147‑1 (MQ=255)
    ttCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCAc                                                                                                                                               <  2:471903/149‑1 (MQ=255)
     tCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACg                                                                                                                                              >  1:4667/1‑149 (MQ=255)
       cagcagGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCa                                                                                                                                            >  1:1008203/1‑149 (MQ=255)
               cAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGc                                                                                                                                    >  1:712173/1‑149 (MQ=255)
                     gagaAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCCCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAAGTCACGCAGGCGCAGCTGACGa                                                                                                                              >  2:1036126/1‑149 (MQ=255)
                      agaAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGTTAACAATCAGGCCGCCGAGGTCACGCAGGACCAGCTGACGAg                                                                                                                             >  1:840512/1‑149 (MQ=255)
                      agaAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAg                                                                                                                             >  1:805326/1‑149 (MQ=255)
                        aaaTATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCa                                                                                                                           <  1:396459/149‑1 (MQ=255)
                          atatGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAAt                                                                                                                         <  1:589995/149‑1 (MQ=255)
                           tatGGCTGATTTGAATACGCGCACGGTCCTGACGCCCCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGGGTTCACGCGGGCGCAGCTGACGAGCAAtc                                                                                                                        >  2:190291/1‑149 (MQ=255)
                           tatGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAAtc                                                                                                                        >  2:964933/1‑149 (MQ=255)
                              ggCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCa                                                                                                                     <  1:75392/149‑1 (MQ=255)
                                cTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATc                                                                                                                   >  2:857421/1‑149 (MQ=255)
                                  gATTTGAATACGCGCCCGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCgg                                                                                                                 <  2:1143440/149‑1 (MQ=255)
                                     ttGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGAAGCTGACGAGCAATCTCATCGGCAg                                                                                                              <  2:612669/149‑1 (MQ=255)
                                       gAAGACGCCCACGGTCCTGACGAACCGCTTCACGCAGACGGTTTTCTCCCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCt                                                                                                            <  1:173470/149‑1 (MQ=255)
                                         aTACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAAGCTCATCGGCAGCTTc                                                                                                          >  2:990783/1‑149 (MQ=255)
                                          tACGCGCACGGTCCTGACGCACCGCTTACCCCTGTCATTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCCCATCGGCAGCTTCg                                                                                                         >  2:296161/1‑149 (MQ=255)
                                           aCGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGCGGCGTACTGGCGTCATGTCGATGAAGTCGATAGCAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGa                                                                                                        <  2:1142846/149‑1 (MQ=255)
                                             gcgcACGGTCCTGCCGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAgg                                                                                                      <  2:1066102/149‑1 (MQ=255)
                                             gcgcACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAgg                                                                                                      >  1:437409/1‑149 (MQ=255)
                                               gcATGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGtt                                                                                                    <  2:398021/149‑1 (MQ=255)
                                                cACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGCGCAATCTCATCGGCAGCCTCGAGGttt                                                                                                   >  1:155713/1‑148 (MQ=255)
                                                  cGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgt                                                                                                 <  1:100222/149‑1 (MQ=255)
                                                  cGGTCCTCACGCACCGCTCCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgt                                                                                                 <  2:145743/149‑1 (MQ=255)
                                                     tCCTGCCGCACCGCTTCACGCAGTCGGATTTCTACCGCACGCGGGTGGCGTACAGCCGTCATGTCGAAGAAATAGATAACAATAAGGCCGCCGAGTTCACGCCGGCGCAGCTGACGAGCAATCTCATCGGCAGCCTTCAGGTTATTGtt                                                                                              >  1:83020/1‑149 (MQ=255)
                                                          aCGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTAACGCAGGCGCAGCTGACGAGCAATCTCCTCGGAAGCTTCGAGGTGAGTGTTaaaag                                                                                         >  1:1062569/1‑147 (MQ=255)
                                                            gCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGCTGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCCATCTCCTTAGAAGAGTCGAAGTTCGTGTTAAAcacg                                                                                       >  1:252013/1‑149 (MQ=255)
                                                            gCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCGATCTCATCGGCAGCCTCGAGGCTAGTGTTAAAcgcg                                                                                       >  1:170802/1‑149 (MQ=255)
                                                            gCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAAcgcg                                                                                       >  1:192831/1‑149 (MQ=255)
                                                              aCCGCTTCACGCAGACGGCTTTCTACCGCACGCTGGTGGCGTCATGGGGTCATATCGCTCAAGCCTATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGGTAGTGTTAAACGCggg                                                                                     >  2:895312/1‑148 (MQ=255)
                                                                   ttCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttctt                                                                                <  1:997363/149‑1 (MQ=255)
                                                                   ttCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttctt                                                                                >  2:37675/1‑149 (MQ=255)
                                                                   ttCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGGttctt                                                                                >  2:48137/1‑149 (MQ=255)
                                                                    tCACGCAGCCGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGATATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTCAACTCAGTCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttcttc                                                                               <  2:712173/149‑1 (MQ=255)
                                                                    tCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttcttc                                                                               <  2:514913/149‑1 (MQ=255)
                                                                          aGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATc                                                                         <  2:167044/149‑1 (MQ=255)
                                                                           gACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATcg                                                                        >  2:548749/1‑149 (MQ=255)
                                                                            aCGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATcgc                                                                       >  2:674282/1‑149 (MQ=255)
                                                                                 tttCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATcgccgccg                                                                  >  2:469541/1‑149 (MQ=255)
                                                                                    cTACCGGACGCTGGTGGTGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCAGGGGTAACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCgg                                                               <  2:163251/149‑1 (MQ=255)
                                                                                    cTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCgg                                                               <  2:629962/149‑1 (MQ=255)
                                                                                     tACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCggg                                                              >  1:292370/1‑149 (MQ=255)
                                                                                     tACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGCTTCTTCGATAGCGCCGCGGCggg                                                              >  1:292358/1‑149 (MQ=255)
                                                                                          cACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTcgcg                                                         >  2:931085/1‑149 (MQ=255)
                                                                                            cGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTcgcgcg                                                       <  2:1043580/149‑1 (MQ=255)
                                                                                              cTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTg                                                     >  1:274642/1‑149 (MQ=255)
                                                                                                ggtggCTTACTTTCGGCATGGCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCg                                                   <  2:221245/149‑1 (MQ=255)
                                                                                                ggtggCGTACTGGCGTCAGGCCGATGAAGTCGATAACAATCAGGCCGCCGAGTACACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCg                                                   <  2:547799/149‑1 (MQ=255)
                                                                                                  tggCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGa                                                 >  1:381575/1‑149 (MQ=255)
                                                                                                  tggCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGa                                                 <  1:857421/149‑1 (MQ=255)
                                                                                                     cGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGtt                                              <  2:1104295/149‑1 (MQ=255)
                                                                                                       tACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGa                                            <  1:962849/149‑1 (MQ=255)
                                                                                                       tACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGAGCGTGCGGAGTTGa                                            >  1:740814/1‑149 (MQ=255)
                                                                                                        aCTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGAt                                           <  1:1023909/149‑1 (MQ=255)
                                                                                                          tGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttcttc                                                                               >  2:243224/1‑111 (MQ=255)
                                                                                                          tGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttcttc                                                                               <  1:243224/111‑1 (MQ=255)
                                                                                                          tGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGt                                         >  1:1186196/1‑149 (MQ=255)
                                                                                                          tGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGt                                         >  1:1186173/1‑149 (MQ=255)
                                                                                                            gCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCg                                       >  1:29413/1‑149 (MQ=255)
                                                                                                            gCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCg                                       <  1:407580/149‑1 (MQ=255)
                                                                                                                cATGTCGATGAAGTCGATAACAATCAGGCCGCCGGGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATgg                                   <  1:368042/149‑1 (MQ=255)
                                                                                                                 aTGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGc                                  >  2:1177756/1‑149 (MQ=255)
                                                                                                                  tGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGcc                                 >  1:946155/1‑149 (MQ=255)
                                                                                                                  tGTCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGcc                                 >  1:946147/1‑149 (MQ=255)
                                                                                                                    tCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGt                               <  2:4667/149‑1 (MQ=255)
                                                                                                                    tCGATGAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGAGGGTGCGGGGTTGATGTCGATGGCCGt                               >  2:548781/1‑149 (MQ=255)
                                                                                                                         gAAGTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGtgcagc                          >  1:304861/1‑144 (MQ=255)
                                                                                                                          cagccGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGTGTGCGGAGTTGATGTCGATGGCCGTTAACGc                         <  1:1091917/145‑1 (MQ=255)
                                                                                                                          aaGGCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGc                         <  2:31548/149‑1 (MQ=255)
                                                                                                                            gTCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGCGCGGAGTTGATGTCGATGGCCGTTAACGCtt                       >  1:1047392/1‑149 (MQ=255)
                                                                                                                             tCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTc                      <  2:381156/149‑1 (MQ=255)
                                                                                                                             tCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTc                      >  1:199849/1‑149 (MQ=255)
                                                                                                                             tCGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGGCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTc                      >  1:626568/1‑149 (MQ=255)
                                                                                                                              cGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCg                     <  2:292532/149‑1 (MQ=255)
                                                                                                                              cGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCg                     <  2:805326/149‑1 (MQ=255)
                                                                                                                              cGATAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCg                     >  2:523511/1‑149 (MQ=255)
                                                                                                                                aTAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGt                   <  2:1199031/149‑1 (MQ=255)
                                                                                                                                aTAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAAGCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGGGCGGAGTTGATGTCGATGGCCGGTAACGCTTCGGt                   >  1:914237/1‑149 (MQ=255)
                                                                                                                                aTAACAATCAGGCCGCCGAGTTCACGCACGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGt                   >  2:673840/1‑149 (MQ=255)
                                                                                                                                 tAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCGGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTg                  >  1:800699/1‑149 (MQ=255)
                                                                                                                                 tAACAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTg                  <  2:689705/149‑1 (MQ=255)
                                                                                                                                   accaTCAGCCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAAGCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCt                <  2:59072/146‑1 (MQ=255)
                                                                                                                                   aCAATCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCt                <  2:274642/149‑1 (MQ=255)
                                                                                                                                     aaTCAGGCCGCCGAGTTCACGCGGGCGCAGCTAGCGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTATTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGt              <  1:687162/149‑1 (MQ=255)
                                                                                                                                     aaTCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGt              <  2:98216/149‑1 (MQ=255)
                                                                                                                                     aaTCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGt              <  2:98343/149‑1 (MQ=255)
                                                                                                                                       tCAGGCCGCCGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCg            >  1:454416/1‑149 (MQ=255)
                                                                                                                                           gccgccGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATaa        <  1:964933/149‑1 (MQ=255)
                                                                                                                                           gccgccGAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATaa        >  2:102454/1‑149 (MQ=255)
                                                                                                                                           cccgccgAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGGTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGCGTTAATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATaa        <  2:1020883/148‑1 (MQ=255)
                                                                                                                                               ccgAGTTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGGTGGCCGTTAACGCTTCGGTGCTGGCGATAACAAt    >  2:793836/1‑149 (MQ=255)
                                                                                                                                               ccgAGTTCACGCAGGCGCAGCTGACGAGCAATATCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCAGTTAACGCTTCGGTGCTGTCGATAACAAt    <  1:674040/149‑1 (MQ=255)
                                                                                                                                                cgAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATg   <  2:29413/149‑1 (MQ=255)
                                                                                                                                                 gAGTTCCCGCAGGCGCAACTGAAGAGAAATCTCAACGGCAGCTTCGCGGTTAGTGTTAAACGCGGTGTCTTCGATATCGCCGCCGCGGGTCGGGGGTGAGGAGGTGAGGACGATGGCCTGTAACGCATCGGTGCTGTCGATAACAATgg  >  1:269440/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATGG  >  CP009273/1138662‑1138955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑