| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,131,672 | Δ1 bp | 16.7% | coding (210/1860 nt) | gss ← | fused glutathionylspermidine amidase/glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,131,671 | 0 | A | . | 16.7% | 62.8 / 9.4 | 24 | coding (211/1860 nt) | gss | fused glutathionylspermidine amidase/glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (8/12); new base . (2/2); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
CAGATAAGAAGCGCACCCGCGACCGGCGCACGCGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTA > CP009273/3131523‑3131814 | cAGATAAGAAGCGCACCCGCGACCGGCGCACGCGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCGCAACGGGCATCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTa > 2:207936/1‑149 (MQ=255) ccGCGACCGGCGCACGCGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGCAAGAGaaaa > 2:76953/1‑149 (MQ=255) aCCGGCGCACGCGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCg > 2:223675/1‑149 (MQ=255) cgcACGGGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCaa < 2:357529/149‑1 (MQ=255) cgcACGCGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCaa > 2:176753/1‑149 (MQ=255) aaaTGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGT‑ATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGcaa > 1:292368/1‑141 (MQ=255) aaaTGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGT‑ATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCa > 1:204698/1‑143 (MQ=255) tGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAACAAATTCAACGCATTGCCACTTGTGGc < 2:266389/149‑1 (MQ=255) tGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCat < 2:216337/149‑1 (MQ=255) gCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTgg < 1:139433/143‑1 (MQ=255) aTGTTGTCATGAACCAGTTCTCGCAGGAAGCGCGGCGAGAAAATCGCCCACGCCATACCCACGTCAGTAAAGCCCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCATCGCATTGCCACTTGTGGCCCATATATtc < 1:186372/143‑1 (MQ=255) tgttgtCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTTCCACTTGTGGCCCATATATtcgtcgtcg < 2:239138/149‑1 (MQ=255) gttgtCATTAACCACTTCGCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGa < 2:67344/149‑1 (MQ=255) aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTAc > 2:165535/1‑149 (MQ=255) cACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGT‑ATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGaa < 2:328469/149‑1 (MQ=255) cACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGT‑ATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACCTGTGGCCCATATATTCGTCGTCGATATAGCTACGGat < 2:234121/149‑2 (MQ=255) cAGGACGCGCCGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATa < 1:390280/143‑1 (MQ=255) aGGAAGCGCAGCGAGAAAATCTCCCACGCCAGACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCAATGCAACGTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTc < 2:231046/149‑1 (MQ=255) aGCGCAGCGAGAAAATCTCCCACGCCATACCCACGGCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCACCGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCg < 1:253561/143‑1 (MQ=255) tcCCACGCCATACCCACGCCCGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTg < 1:216787/143‑1 (MQ=255) aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCgaga > 2:30613/1‑149 (MQ=255) ccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATGCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGaa > 1:204125/1‑143 (MQ=255) cACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCGTCTTCGTATTCCTGCGGATCGAGAGCACTGTAAt > 2:76400/1‑149 (MQ=255) aaGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCCCTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTa < 2:406892/149‑1 (MQ=255) acCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTa > 2:77701/1‑149 (MQ=255) | CAGATAAGAAGCGCACCCGCGACCGGCGCACGCGGCGAGCCGTTAGGAAATGCCTGCAATGGCAGGATGTTGTCATTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTA > CP009273/3131523‑3131814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |