Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,084,016 Δ1 bp 25.0% coding (344/900 nt) hisG → ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,084,0160C.25.0% 33.9 / 10.6 16coding (344/900 nt)hisGATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (8/4);  new base . (2/2);  total (10/6)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGC  >  CP009273/2083886‑2084150
                                                                                                                                  |                                                                                                                                      
aTCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCAGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTcc                                                                                                                            >  1:61023/1‑143 (MQ=255)
 tCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACTCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTcc                                                                                                                     >  2:192254/1‑149 (MQ=255)
  cGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGCGTGCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTa                                                                                                                  >  2:101861/1‑149 (MQ=255)
                              ctTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTa                                                                                        >  2:360280/1‑149 (MQ=255)
                                                      gATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTAtct                                                                >  2:398541/1‑149 (MQ=255)
                                                       aTCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTa                                                                   >  1:114894/1‑143 (MQ=255)
                                                        gccACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGtt                                                                    <  1:339181/142‑1 (MQ=255)
                                                                  ttttCCCGGCGTCGGCTGGATTTCGGCGGCTTTCGTCTTTAGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCGAGCGTTATCTCGACCAGa                                                        <  1:140029/143‑1 (MQ=255)
                                                                  tttACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCa                                                          >  1:22049/1‑143 (MQ=255)
                                                                                 cTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTACCCGCACCAGAAAGGCATCTCttt                                           >  1:17602/1‑143 (MQ=255)
                                                                                  gggAGTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtttt                                          <  1:167998/142‑1 (MQ=255)
                                                                                              gcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGCGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTa                              <  1:350633/143‑1 (MQ=255)
                                                                                                tGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTGTCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGCGATCTCTTTTAAATCCTGCTTCCt                            >  1:15013/1‑143 (MQ=255)
                                                                                                     tcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACg                       >  1:126797/1‑143 (MQ=255)
                                                                                                       ttgcGCTGGCAACGCCGGTTGATGATGCCTGGGACGGTCCGCGCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCCCCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGtt               <  2:226171/146‑1 (MQ=255)
                                                                                                                  accccGGTTGATGAAG‑‑TGGGACGGTCCGCTCCCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgc  <  2:130503/146‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
ATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGC  >  CP009273/2083886‑2084150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: