| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,419,720 | T→C | 28.4% | D183G (GAT→GGT) | hisJ ← | histidine/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,419,720 | 0 | T | C | 28.4% | 70.7 / 41.6 | 60 | D183G (GAT→GGT) | hisJ | histidine/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (19/24); new base C (10/7); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.15e-01 | |||||||||||
AGTGCTTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GTGGTGCCCTGCAATACGCCTACCCGT > CP009273/2419573‑2419866 | aGTGCTTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATc > 2:26403/1‑149 (MQ=255) tCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGaa > 2:375150/1‑149 (MQ=255) gcgcAGTCCCTTATCATCCTTACGCAGGCCCAGGCCGGTCCCAACGCCAAACAGTTTTGCATCTTTAACAGACGGGCCACCGAATTTCAAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAAcg < 2:199739/149‑1 (MQ=255) cAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCgg < 2:278650/149‑1 (MQ=255) gTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCa < 2:112900/149‑1 (MQ=255) gTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTGTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGATCTCATCCTGGAACGCGGCa < 2:315327/149‑1 (MQ=255) ttCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGGCGGGCCCCCGAATTTGTAATCTTGACCGGCAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGACCGCGGcgt > 2:11274/1‑147 (MQ=255) ttCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGGCCTCACCCTGGAACGAGGcct > 2:202715/1‑147 (MQ=255) cGTTATCTCCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAAcgcg < 1:265732/143‑1 (MQ=255) gTTATCTTCCTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCaa < 2:375326/149‑1 (MQ=255) ttATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGc < 1:113845/143‑1 (MQ=255) tcttctTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGCCAGGTTGTGTGGGGAAACCTTCGCTGGCAGCGACCTCATCCGGGAACGCGGCATCAATACg > 2:316294/1‑149 (MQ=255) ttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACGGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCaa > 1:291325/1‑143 (MQ=255) tcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACTATATTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGAGCTCATCCTGGAACGCGGCATCAATACGTcc < 2:226523/149‑1 (MQ=255) tttACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCgg > 2:200581/1‑149 (MQ=255) tACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCa > 2:287822/1‑149 (MQ=255) tACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCa < 2:334754/149‑1 (MQ=255) cGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgt < 2:332201/149‑1 (MQ=255) gCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggt > 2:222100/1‑149 (MQ=255) ccATGCCGGTCCCTCCGCCAAACAGTTTTTCATCTTTAACAGGCGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggtca < 2:363159/149‑1 (MQ=255) gCCGGGCCCGACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggt < 1:90994/143‑1 (MQ=255) ccccgTCCCTACGCCAAACCGCTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAAt < 2:28532/145‑1 (MQ=255) tACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtgttgt < 2:203111/149‑1 (MQ=255) tACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACATCCGGCAGTCAGGTCAGAATaaa > 1:243155/1‑143 (MQ=255) aCGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAt > 1:237443/1‑143 (MQ=255) aCGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTc < 2:238910/149‑1 (MQ=255) aCGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAt < 1:253831/143‑1 (MQ=255) cGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtg > 1:312495/1‑143 (MQ=255) tttCATCTTTAACAGACGTGCCACCGAATTTGTAATCTTTACCGCCAGGTTGTTTGAGGAACCCTTCGCTGGCAGCGGCCTTACCCTGGAACGCGGCATCAAGACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGcccc < 1:163950/143‑1 (MQ=255) cATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGa < 1:222785/143‑1 (MQ=255) ttAACAGACGGGTCACCGAATTTTGAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACga < 1:329621/143‑1 (MQ=255) aCAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTc > 2:76842/1‑149 (MQ=255) cAGACGGGCCACCGAATTAGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCa > 2:319637/1‑149 (MQ=255) aGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGTGGAAGCCGTCGCTAGCAAAGTCCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATTTTGTCCTGCCCCTGATACGAGGCGATTTCaa > 2:103310/1‑149 (MQ=255) gACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAAt > 2:161401/1‑149 (MQ=255) gACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAt < 1:250414/143‑1 (MQ=255) aCGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATg > 2:20599/1‑149 (MQ=255) gggCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTc < 1:66287/143‑1 (MQ=255) aCCGAATTGGTAATCTTTTCCGATAGGTTGTTTGAGGAAACCTTCGCTGGAGGGTGGCTCATCCTGGACCGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATCAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTg < 2:83579/149‑1 (MQ=255) aTTTGTAATCTTTACCGACAGGTTGTTTGACGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCTGGTCAGAATAAATGTTGTCATGCCCCTTATACGAGACGATTTCAATGCCTTTTGGTGcg > 2:225983/1‑148 (MQ=255) tCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCa > 1:24753/1‑143 (MQ=255) cGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGCCCATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCg > 2:171495/1‑149 (MQ=255) gttgttTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGCCGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGtc > 2:402214/1‑149 (MQ=255) gttgttTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGtc > 2:252411/1‑149 (MQ=255) tgttTGAGGAAACCTTCGCGGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTAAGGTCAGAATTAATGTTGTCCTGCCCCTGATGCGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGaa < 1:329380/143‑1 (MQ=255) ggtgtGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCTATACGTCCGGCAGTCAGGTCAGAATAACTGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGaa < 1:237297/139‑1 (MQ=255) gAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGg‑gtgg > 2:49594/1‑149 (MQ=255) aaaCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTg > 1:377441/1‑143 (MQ=255) aaCCTTCGCTGGCAGCGACCTCACCCTGGGACGCGGCATCAATACGTACGACAGTCAGGTAAGAATAAATGTTGGCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCATTGTTCATTAGCGACAGGCTCCTGG‑gcggtg > 2:50183/1‑149 (MQ=255) ccctCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGCCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GTGGTGcc < 2:232173/146‑1 (MQ=255) ttCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGGCTCCTGG‑GTGGTGCCCt > 2:95829/1‑149 (MQ=255) tCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GTGGTGCCCTg < 2:351435/149‑1 (MQ=255) tCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GTGGTGCCCTg < 2:157635/149‑1 (MQ=255) tGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GTGGTGCCCTGCAAt > 2:203175/1‑149 (MQ=255) cgCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GTGGTGCCCTGCAATa < 2:246147/148‑1 (MQ=255) gCAGCGACCTCATCCTGGAACGCGGCTTCAATACGTCCGGCAGTCAGGCCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCTATGCCTTTTGGTGCCCAATGTTCCTTACCGAACGTCTCCTGG‑GTGGTGCCCTg < 1:208964/143‑1 (MQ=255) aGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGTTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCACCTCG‑GCGGTGCCCTgta > 1:93333/1‑141 (MQ=255) gACCTCATCCGGGAACGCGGCATCAATCCGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GTGGTGCCCTGCAATa < 1:118170/143‑1 (MQ=255) cTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAACTGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GGGGTGCCCTGCAATACGc > 1:98674/1‑143 (MQ=255) cATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCAATCG‑CTACTGGTTTGGTGCCCTGCAATACGCCTACCCGt < 2:320656/149‑1 (MQ=255) | AGTGCTTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGG‑GTGGTGCCCTGCAATACGCCTACCCGT > CP009273/2419573‑2419866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |