| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,705,098 | G→A | 15.4% | W440* (TGG→TGA) | nadB → | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,705,098 | 0 | G | A | 15.4% | 56.1 / 4.7 | 26 | W440* (TGG→TGA) | nadB | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Reads supporting (aligned to +/- strand): ref base G (12/10); new base A (2/2); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-01 | |||||||||||
TTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGA > CP009273/2704952‑2705244 | ttGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGTCGAACGGGTAGTAATTCAGCATAACTGGCa < 2:127147/149‑1 (MQ=255)ttGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGCACGGGTAGTAATTCAGCATAACTGGCa > 2:415410/1‑149 (MQ=255) tCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGt < 1:113069/143‑1 (MQ=255) tGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGAGGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGa < 2:307908/149‑1 (MQ=255) ggcggcAGATACCCCGTGGCGTATGCCTTATGCCCACGACATCGGTACGGTACGGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGAtt < 1:291058/143‑1 (MQ=255) acaaGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATtgt > 1:357827/4‑143 (MQ=255) ccAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGACACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATtgtg > 1:149262/1‑143 (MQ=255) ccAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGACACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATtgtg > 1:346400/1‑143 (MQ=255) ttATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGGCGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTgg > 2:163387/1‑149 (MQ=255) cACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGGACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGAGTACGTTGGCATTGTGCGCGCAACGAAGCGCCTGGa > 1:126211/1‑143 (MQ=255) cagcAGTACGTTACCGCCGGGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCACTGTGCGCACAACGAAGCGCCTGGAAcgcg < 1:377138/140‑1 (MQ=255) aGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCCTCACTGGCACGAGCTACGTCTGTTTATGCGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCt > 1:16865/1‑143 (MQ=255) tACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCGTTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGa > 2:174902/1‑149 (MQ=255) cgccgTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCCGTGTGCGCACAACGCAGCGCCTGGAACGCGCCCTGCGGCGGATa > 1:68299/1‑143 (MQ=255) gggATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATg < 1:284108/143‑1 (MQ=255) ggATGAAAGCCGCGTTGAGCTCCCTGACGAACGGGTAGTAATTCCGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACACCGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCcaac < 2:97196/149‑1 (MQ=255) aaGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGACACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGaaa < 2:170529/149‑1 (MQ=255) aaGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGACACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTCTGCACACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGaaa < 2:387109/149‑1 (MQ=255) aaCCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTa > 2:291428/1‑149 (MQ=255) ccTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATTCTCCAACAGGAAAGAGACGAata > 1:277656/1‑143 (MQ=255) gACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCa > 2:68302/1‑149 (MQ=255) aaTTCAGCATACGTGGCACGAGCGACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCaa < 2:97659/149‑1 (MQ=255) aTTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCaaa > 2:22259/1‑149 (MQ=255) tCAGCATCACTGGCACGCGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGtct < 1:372780/143‑1 (MQ=255) tCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAAGAGACGAATATTACGCCCATTTCCGCGtcc > 1:329449/1‑142 (MQ=255) tGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGa < 2:359671/149‑1 (MQ=255) | TTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGA > CP009273/2704952‑2705244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |