| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,775,283 | C→T | 17.2% | G268D (GGC→GAC) | ypjA ← | adhesin‑like autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,775,283 | 0 | C | T | 17.2% | 48.0 / 2.7 | 23 | G268D (GGC→GAC) | ypjA | adhesin‑like autotransporter |
| Reads supporting (aligned to +/- strand): ref base C (13/6); new base T (2/2); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATT‑CAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCT > CP009273/2775142‑2775430 | ttGTCTGCCACGTGATTGTGGATGGAGAAGGCACCTTGACTATTCGTACCGCTCACCGTCGTAACGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGa > 2:282661/1‑149 (MQ=255) tGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATaaa < 2:134916/149‑1 (MQ=255) gATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGTCACCAGAATAAACTTCAATa > 2:132920/1‑149 (MQ=255) gATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTGTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCAACCCTAACATCAAGCACGTCACCAGAATAAACTTCCATa > 2:16006/1‑149 (MQ=255) ttGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAATCTTc < 1:232465/143‑1 (MQ=255) gagaATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGt > 2:238280/1‑149 (MQ=255) tCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATca > 1:52771/1‑143 (MQ=255) aCCGTCCTACCGTTAGTGTTAGTTTTTAAAACTCCACCACCGTCCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGt < 1:179566/143‑1 (MQ=255) aCCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTCTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATCACATCCGAGCTGCTGGTGTTAACAACAATTTGCGTGCcagcag > 1:297715/1‑143 (MQ=255) ttagtgttagtTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATg > 2:71315/1‑149 (MQ=255) ttagtTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTAtt > 2:275530/1‑149 (MQ=255) tttttAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCCCCAGACTAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACACTTTGCGTGCCACCAGGATGGATATGCGtc > 1:282528/1‑142 (MQ=255) tttAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGc > 2:433299/1‑149 (MQ=255) ccATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGTCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGa < 1:116969/143‑1 (MQ=255) ccATCGTGCTGCGTAATATTTGTTGCCGCACCACCCCAAACATCAAGCACGTCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGa < 1:13761/143‑1 (MQ=255) tGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCAGCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGACACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATT‑Cag > 2:272517/1‑149 (MQ=255) tgtgTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTAtt < 1:187911/140‑1 (MQ=255) tGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTAtt < 1:195744/143‑1 (MQ=255) ggagTAACACCCCTAACATCAAGCACGCAACCAGAATAAACTTAAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCAACAAGAATAGATATGCGTATTTTTTGCAGTTGACTTACTATT‑CAAAGGCTGAGTTcc > 1:323879/4‑143 (MQ=255) ccccTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTTTCGTTTACTTACTATT‑CAGAGACTGACTTCCGCCTTCAAt > 1:238474/1‑143 (MQ=255) cATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATT‑CAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAgc > 2:55537/1‑149 (MQ=255) cGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATT‑CAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCa < 1:63156/143‑1 (MQ=255) gCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTACAGGGGC‑GTGTTCTGCCTTCAATCGTCGTGTACAGCGCAGGGctcg > 2:335654/1‑148 (MQ=255) | TTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATT‑CAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCT > CP009273/2775142‑2775430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |