Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	982,560	T→A	intergenic (‑122/+481)	ompF ← / ← asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	982,560	0	T	A	100.0%	193.5 / NA	58	intergenic (‑122/+481)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (28/30);  total (28/30)

TGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTA  >  CP009273/982415‑982703                                                                                                                                                  |                                                                                                                                                ggCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCa                                                                                                                                              <  1:780026/148‑1 (MQ=255)   ccAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAtt                                                                                                                                            >  1:1026436/1‑149 (MQ=255)       aaTATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTTTCTGCAGGCATCTTACCATTCaaa                                                                                                                                        <  1:916595/149‑1 (MQ=255)                 ttCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTc                                                                                                                              >  2:948614/1‑149 (MQ=255)                 ttCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTc                                                                                                                              >  1:97936/1‑149 (MQ=255)                      catTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCCTTCAAACTAACGTTTCGCTAc                                                                                                                         >  2:57556/1‑149 (MQ=255)                             tattcCCCTGCTGGTTTTTTTTATGACACCGGCCACGGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGCCTGCAGGCATCGTACCATTCAAACTAACGTTTCGCTACCGTGaaa                                                                                                                  <  2:513418/149‑1 (MQ=255)                             tattaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGGCAATAAAAATTTACGGAACTATTGATGAGAGTGTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGaaa                                                                                                                  <  2:420403/149‑1 (MQ=255)                             tagtaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGaaa                                                                                                                  <  2:229320/146‑1 (MQ=255)                             tactaCCCTCCTGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAACATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGaaa                                                                                                                  <  1:832764/146‑1 (MQ=255)                              attaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAATTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATGTTACCATCAAACCTAACGTTTCGCTCCCGTGTAAg                                                                                                                 >  2:464099/1‑149 (MQ=255)                              attaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGACAATAAGTTCTGTCTATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATATGTCGCCAGGCATCTTACCATTCAAACTAACGTTTTGCTAccattacag                                                                                                                 >  2:419887/1‑142 (MQ=255)                               ttaCCCTCATGGTTTTTTTTATTACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGt                                                                                                                <  2:730817/149‑1 (MQ=255)                                tcccccCATCGTTTTTTTTATCACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTg                                                                                                               <  2:932924/147‑1 (MQ=255)                                  cccTCATGGTTTTTTTTTTTTTTTTTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTTAAACTAACGTTTCGCTACCGTGTAAGTGCt                                                                                                             >  2:988775/1‑149 (MQ=255)                                   ccTCGTGGTTTTTTTTATGACACCTGCCACTGCCGTCAATTAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTa                                                                                                            <  2:1064624/149‑1 (MQ=255)                                     tCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGCAAGTGCAACa                                                                                                          >  2:551779/1‑149 (MQ=255)                                      cATGGTTTTTTTTATGACACCTGCCACTGCCGTCACTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGTTCCCGTGAAAGTGCTACaa                                                                                                         >  2:674364/1‑149 (MQ=255)                                          gTTTTTTTTATGACACCTGCCACTGCCGACAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGGGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAACCTAACGTTTCGCTACCGTTAAAGTGCTACaaagat                                                                                                     >  2:85536/1‑149 (MQ=255)                                           ttttttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagata                                                                                                    >  1:1018024/1‑149 (MQ=255)                                           ttttttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagata                                                                                                    >  1:1017797/1‑149 (MQ=255)                                                    tcacacCAGCCACGGCCGTCAAAAAGGTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGCttatt                                                                                           <  2:417312/147‑4 (MQ=255)                                                          cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa                                                                                     <  2:42001/149‑1 (MQ=255)                                                          cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa                                                                                     >  1:518752/1‑149 (MQ=255)                                                            gCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa                                                                                   <  2:206425/149‑1 (MQ=255)                                                            gCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa                                                                                   <  1:1033841/149‑1 (MQ=255)                                                              cACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAg                                                                                 <  1:571983/149‑1 (MQ=255)                                                              cACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCAGAAAAGTGCTACAAAGATAAAGATTTTGTTTCAAAAAg                                                                                 >  2:173372/1‑149 (MQ=255)                                                                 tGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaa                                                                              >  2:1113996/1‑149 (MQ=255)                                                                    cGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaaa                                                                           <  1:540582/149‑1 (MQ=255)                                                                      ccAATAAGTTCTTTCAATAAAAGTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAata                                                                         <  2:405119/148‑1 (MQ=255)                                                                       cAATAAGTTCTGGCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAatat                                                                        <  1:728501/149‑1 (MQ=255)                                                                        aaTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATg                                                                       >  1:188741/1‑149 (MQ=255)                                                                           aaGTTCTGCCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACCAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTaa                                                                    <  2:674050/149‑1 (MQ=255)                                                                                ccgTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaaa                                                               <  1:1222730/147‑1 (MQ=255)                                                                                cTGTCAATAACAATTTAACGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCCTTCAAAATCACGTTTCGCTACCGTGCACGTGCTACAAAGATAAAGACTTAGTTTCAAAACGAAAAACTATGTAACCaaa                                                               >  2:505803/1‑149 (MQ=255)                                                                                 tGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGACGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaaaa                                                              <  1:98760/149‑1 (MQ=255)                                                                                     aaTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAGTATGTAACCAAAAGTaa                                                          >  2:448477/1‑149 (MQ=255)                                                                                     aaTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaa                                                          >  1:958541/1‑149 (MQ=255)                                                                                            aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa                                                   <  1:1101842/149‑1 (MQ=255)                                                                                            aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGGGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa                                                   <  1:312597/149‑1 (MQ=255)                                                                                                  ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACt                                             >  2:787785/1‑149 (MQ=255)                                                                                                    aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAGAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACttt                                           >  1:953306/1‑149 (MQ=255)                                                                                                     accaTTGAGGATAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACGAACGTTGCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtg                                          <  2:492834/146‑1 (MQ=255)                                                                                                       tATTGATAAGAGTATGGTGTCGTTATGTGTCTGCAGGCAACTAACCATTCAAACTAACGTGTCGCTACCGTGAAAGTGCTACAACCATAAAGTTTTGGAAAAAAAAAAAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg                                        <  2:1013314/149‑1 (MQ=255)                                                                                                                gagTTTGGTGGCTTTATGTGTCTGCAGGCATCTTACCAATCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTc                               <  2:661378/149‑1 (MQ=255)                                                                                                                 agTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAATAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCa                              <  2:600190/149‑1 (MQ=255)                                                                                                                    ttGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat                           >  2:547383/1‑149 (MQ=255)                                                                                                                        tgtCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAAATTTGTGAACACCGTCATATTTcc                       >  2:1137131/1‑149 (MQ=255)                                                                                                                            tttATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATag                   >  1:391876/1‑149 (MQ=255)                                                                                                                             ttATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTCCCGGGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAATATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTTCAtcaa                  >  2:560914/1‑146 (MQ=255)                                                                                                                                  tgtCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGt             <  1:465769/149‑1 (MQ=255)                                                                                                                                  tgtCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGt             <  1:1052117/149‑1 (MQ=255)                                                                                                                                    tCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGTTACAGATGTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtga           <  1:1207239/149‑1 (MQ=255)                                                                                                                                    tCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtga           >  1:385369/1‑149 (MQ=255)                                                                                                                                            cATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAttt   >  2:948644/1‑149 (MQ=255)                                                                                                                                             aTCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtttc  >  2:181421/1‑148 (MQ=255)                                                                                                                                             aTCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTa  <  1:861395/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                TGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTA  >  CP009273/982415‑982703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑