Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,305,946 G→T R95S (CGT→AGT)  ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,9460GT98.8% 253.2 / NA 86R95S (CGT→AGT) ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (40/43);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCA  >  CP009273/2305803‑2306094
                                                                                                                                               |                                                                                                                                                    
cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCCCATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTcc                                                                                                                                                 >  2:288061/1‑149 (MQ=255)
    aaGTTGTCAGCACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGa                                                                                                                                             <  2:184764/149‑1 (MQ=255)
    aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACGTCCTGGAATTTCAGACCTGTGAATGCCACTCTGGTc                                                                                                                                                  >  1:761424/1‑144 (MQ=255)
    aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGa                                                                                                                                             <  2:669663/149‑1 (MQ=255)
    aaGTTGTCAGAACCGTAGGTGTCACCACCGAATGCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGa                                                                                                                                             <  2:185053/149‑1 (MQ=255)
      gTTGTCAGAACCGTAGGGGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGTCCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAgt                                                                                                                                           <  2:126595/149‑1 (MQ=255)
       ttGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCGCATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAg                                                                                                                                               <  1:928023/144‑1 (MQ=255)
       ttGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGGCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAg                                                                                                                                               >  1:251334/1‑144 (MQ=255)
         gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGGCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGAGAATGCCACACAGATCACGGAgttat                                                                                                                                        >  2:284403/1‑147 (MQ=255)
         gTCAGAACCGTAGGTGTCACCACCGAATTCGGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGa                                                                                                                                             <  1:448497/144‑1 (MQ=255)
           cAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACCCTGGTCCAGGAgt                                                                                                                                           >  1:413356/1‑144 (MQ=255)
            aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGGCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGCCCAGGAgag                                                                                                                                          >  1:311782/1‑142 (MQ=255)
                  gTAGGCGTCGCCACCGTATTCGGGCAGTACGCAGGTCCAGGAAGTTACGTAATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTTGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTgttttcgtttt                                                                                                                               <  2:73595/149‑1 (MQ=255)
                    aGGGGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCa                                                                                                                             <  2:151172/149‑1 (MQ=255)
                            ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAgcg                                                                                                                          >  1:841962/1‑144 (MQ=255)
                               ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCACTATTTcc                                                                                                                  >  2:844670/1‑149 (MQ=255)
                               ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAACGCTGTTGcc                                                                                                                  >  2:844648/1‑149 (MQ=255)
                                cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGt                                                                                                                      >  1:557243/1‑144 (MQ=255)
                                 gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTTTTGCCCt                                                                                                                >  2:334074/1‑149 (MQ=255)
                                 gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCt                                                                                                                <  2:469125/149‑1 (MQ=255)
                                  aaTTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCACCGCTCCTCCGGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTg                                                                                                               >  2:802567/1‑149 (MQ=255)
                                       tGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCt                                                                                                          >  2:1010179/1‑149 (MQ=255)
                                        ggCAGTACGTCGGTCCAGGTAGTTTCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGGCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTg                                                                                                         <  2:55705/149‑1 (MQ=255)
                                          cAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCGGTTGCCCTGGAt                                                                                                            >  1:349954/1‑144 (MQ=255)
                                           aGTACGTCTGTCCAGGAACTTACGTCATAAACAACGCCGCAGTCACGACCGCAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACCGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGata                                                                                                      <  2:437119/149‑1 (MQ=255)
                                           aGTACGTCGGTCCAGGAAGTTACGCCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGata                                                                                                      <  2:437132/149‑1 (MQ=255)
                                               cggcGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTcc                                                                                                  <  2:355956/146‑1 (MQ=255)
                                                   gggccAGGAAGTTACGTCATAAACAACCCCGTAGTTACGACCGTAGTCGAAAGAACCCACCTCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTc                                                                                                   <  1:755808/141‑1 (MQ=255)
                                                        aGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACt                                                                                              >  1:709487/1‑144 (MQ=255)
                                                          gAAATTACGTCACAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTgg                                                                                            <  1:276495/144‑1 (MQ=255)
                                                            aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCGCATCCTGGAATTGCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGGTTTCGTTTTCAGCGCTGTTGCCCTGGGTCTGATATTCCCACTGGCCGTAAc                                                                                     >  2:34648/1‑149 (MQ=255)
                                                               tACGGCATAAACAACGCCGTAGTTACGCCCGTAGTCGAAAGAACCCACATCCTCGCATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGGTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTa                                                                                       <  1:738089/144‑1 (MQ=255)
                                                                aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTaa                                                                                      >  1:734520/1‑144 (MQ=255)
                                                                aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTaa                                                                                      <  1:109051/144‑1 (MQ=255)
                                                                  gTCATAAACAACGCCGTAGTTACTACCCTAGTCGAAAGAACCCACATCCTGGAATTTCACACCTGCGAATGCCACACTGGCCCAGGAGTTGCTTCCGTTTTCAGCGCCGTTGCCCTGGATCTTATCTTCCCACTGGCCGCAAcc                                                                                    <  1:259401/144‑1 (MQ=255)
                                                                   tCATAAACAACGCCGTGGTTACCACCGTAGCCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCg                                                                                   <  1:948613/144‑1 (MQ=255)
                                                                   tCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCg                                                                                   >  1:838009/1‑144 (MQ=255)
                                                                   tCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCg                                                                                   <  1:529491/144‑1 (MQ=255)
                                                                    cATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCgg                                                                                  >  1:133920/1‑144 (MQ=255)
                                                                       aaaCAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAAACGGTCAGCTgg                                                                          >  2:280272/1‑149 (MQ=255)
                                                                        aacaatGCTTTAGTTACGATCGTAGTCGAAAGAACCCACCTCCTGGAATTTCAGACCTGCGCATGCTACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGt                                                                         <  2:19249/149‑1 (MQ=255)
                                                                           aacGCCGTAGTTACGACCTTAGTCGAAAGAACCCACATCCTGGACGTACAGACCTGAGAATTCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAg                                                                      >  2:763787/1‑149 (MQ=255)
                                                                           aacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGTAAAACAGACCTGAGAATGACTCACGGGGCCAGGAGGTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCAACTGGCCGTAACCGGTCAGCTGGGCAg                                                                      >  2:763933/1‑149 (MQ=255)
                                                                              gCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTc                                                                        >  1:143270/1‑144 (MQ=255)
                                                                              gCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCAGCTGGCCGTAGCCGGTAGGCTgagc                                                                        >  1:543992/1‑141 (MQ=255)
                                                                               ccGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCCGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCa                                                                       >  1:421439/1‑144 (MQ=255)
                                                                                cGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAg                                                                      >  1:27416/1‑144 (MQ=255)
                                                                                cGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAg                                                                      >  1:883635/1‑144 (MQ=255)
                                                                                     ttACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCCCTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGGGt                                                            >  2:846454/1‑149 (MQ=255)
                                                                                      tACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACGCTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCt                                                                >  1:673447/1‑144 (MQ=255)
                                                                                       aagACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGttt                                                          <  2:391106/147‑1 (MQ=255)
                                                                                       aCGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGttt                                                          <  2:779828/149‑1 (MQ=255)
                                                                                          cccGTAGTCGAAAGAACCCACACCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTTGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCAc                                                       <  2:274988/148‑1 (MQ=255)
                                                                                             gTAGTCGAAAGACCCCACACCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCtt                                                    <  2:16311/149‑1 (MQ=255)
                                                                                             gTAGTCGAAAGAACCCACAGACTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCtt                                                    <  2:16360/149‑1 (MQ=255)
                                                                                              tAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCttt                                                   <  2:948360/149‑1 (MQ=255)
                                                                                                       gAACCCACATCCTGGAATTTTAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAg                                               <  1:761302/144‑1 (MQ=255)
                                                                                                        aaCCCACATCCTGGAATTTCAGACCTGCGAATGCCACACTGCTCCAGGACTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACATTTGAAGCCAAGa                                         >  2:746600/1‑149 (MQ=255)
                                                                                                           ccgccgcttGGCATTTCAGACCTGCGAATGGCACGCTGGTCGAGGAGGTGTTTTCGTTTTCAGCGCTGTTGCCCGGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTCACCTGAGTTTCACCTTTGAAGCCaa                                           <  1:668758/136‑1 (MQ=255)
                                                                                                           ccACATCCTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGc                                      >  2:881312/1‑149 (MQ=255)
                                                                                                                tCCTGTAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGc                                      <  1:95977/144‑1 (MQ=255)
                                                                                                                 ccgggAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCATCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGGAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAg                                <  2:913222/146‑1 (MQ=255)
                                                                                                                 ccTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTATCGTTGGCTTCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAg                                <  2:458532/149‑1 (MQ=255)
                                                                                                                  cTGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAgg                               >  2:879222/1‑149 (MQ=255)
                                                                                                                   tGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATg                                   >  1:877058/1‑144 (MQ=255)
                                                                                                                   tGGAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATg                                   >  1:260983/1‑144 (MQ=255)
                                                                                                                    ggAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTc                             >  2:749890/1‑149 (MQ=255)
                                                                                                                     gAATTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTa                                 >  1:66049/1‑144 (MQ=255)
                                                                                                                      aaTTTCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTg                           <  2:742233/149‑1 (MQ=255)
                                                                                                                        tttCAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGt                              >  1:545432/1‑144 (MQ=255)
                                                                                                                         ttCAGACCTGCGATTGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTc                        <  2:828508/149‑1 (MQ=255)
                                                                                                                           cAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGc                      <  2:615114/149‑1 (MQ=255)
                                                                                                                           cAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCCCTGTTGCCCTGGAGCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTg                           >  1:623939/1‑144 (MQ=255)
                                                                                                                           cAGACCTGCGAATGCCACACTGGTCCAGGAGTTGTTTGCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTg                           <  1:226774/144‑1 (MQ=255)
                                                                                                                               ccTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATc                  <  2:593280/149‑1 (MQ=255)
                                                                                                                                cTGCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGc                      >  1:670110/1‑144 (MQ=255)
                                                                                                                                  gCGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCtcc               >  2:858775/1‑147 (MQ=255)
                                                                                                                                   cGAATGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACa              >  2:741482/1‑149 (MQ=255)
                                                                                                                                   cGAAGGCCACACTGGCCCAGGAGTTGTTTTCGTTTTCCGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACa              <  2:316082/149‑1 (MQ=255)
                                                                                                                                      aTGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATCTTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTa                <  1:699915/144‑1 (MQ=255)
                                                                                                                                      aTGCCACACTGGTCCAGGAGTTGTTTTCGTTTTCACGGCTGTTGCCCTGGATCTGATATTCCCACTGGCCTTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTTAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTa                <  1:700047/144‑1 (MQ=255)
                                                                                                                                        gCCACCCTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCttt         <  2:867753/149‑1 (MQ=255)
                                                                                                                                         ccATACTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttg        <  2:956615/149‑1 (MQ=255)
                                                                                                                                           acacTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgtt      <  2:63053/149‑1 (MQ=255)
                                                                                                                                           acacTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGGCTGGTCGCCATCTACATCTttgtt      <  2:773566/149‑1 (MQ=255)
                                                                                                                                            cacTGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACCTCTttgttg     >  2:250288/1‑149 (MQ=255)
                                                                                                                                               gggTCCAGGAGTTGTTTGCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCa  <  2:387392/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCA  >  CP009273/2305803‑2306094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑