Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,506,454	C→T	intergenic (‑269/‑67)	mntH ← / → nupC	manganese/divalent cation transporter/nucleoside (except guanosine) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,506,454	0	C	T	100.0%	141.9 / NA	43	intergenic (‑269/‑67)	mntH/nupC	manganese/divalent cation transporter/nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/28);  total (15/28)

ATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAA  >  CP009273/2506313‑2506598                                                                                                                                              |                                                                                                                                                 atgaCTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTcc                                                                                                                                           <  2:769764/149‑1 (MQ=255)     cTTGAATGTTATGGTTATGTTTAATGTTAGCACACTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTc                                                                                                                                            <  1:847857/144‑1 (MQ=255)      ttGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTcc                                                                                                                                           >  1:166570/1‑144 (MQ=255)         aaTGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCAGTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCAt                                                                                                                                   <  2:428323/149‑1 (MQ=255)          aTGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTaa                                                                                                                                       <  1:663066/144‑1 (MQ=255)               atggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACa                                                                                                                             <  2:322076/149‑1 (MQ=255)                 ggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTTTATCTTGTCACAGGACGTCATTATAGTGAGTGTCAGCTCTTGTTTTCCTTAACCATGtt                                                                                                                                >  1:401200/1‑144 (MQ=255)                  gttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTa                                                                                                                               <  1:490675/144‑1 (MQ=255)                    tatcTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCACGTTACATAGaa                                                                                                                        <  2:203508/149‑1 (MQ=255)                          tAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCa                                                                                                                  <  2:921872/149‑1 (MQ=255)                           aaTGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGCTCCCTATTTTTGAAGCCTGTCACAGGACGTCATTAAAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATGGAAtg                                                                                                                      >  1:620345/1‑144 (MQ=255)                               ttAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGaa                                                                                                             >  2:18189/1‑149 (MQ=255)                                      aTTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAAc                                                                                                      <  2:135968/149‑1 (MQ=255)                                                  ttCATCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTc                                                                                               <  1:33899/144‑1 (MQ=255)                                                    cAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATCTAACCTGCCTCATATTTg                                                                                        >  2:956278/1‑149 (MQ=255)                                                    cAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTCAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTg                                                                                        <  2:938171/149‑1 (MQ=255)                                                      gCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGa                                                                                      <  2:215248/149‑1 (MQ=255)                                                        ttCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTCTAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATCGAATGTGCACGGAAATTTAACCTGCCTCATCttc                                                                                         >  1:367600/1‑143 (MQ=255)                                                          ccATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTgg                                                                                       <  1:333329/144‑1 (MQ=255)                                                           cATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGa                                                                                      >  1:664525/1‑144 (MQ=255)                                                                  aacaTAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCGGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAGCCATGTTACATAGAATGTGCACGGAAAGTTAACCTGCCTCATATTTGGAGCAActa                                                                               >  1:476242/1‑144 (MQ=255)                                                                   acaTAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGAcc                                                                         >  2:446639/1‑149 (MQ=255)                                                                     aTAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACcgc                                                                       <  2:224565/149‑1 (MQ=255)                                                                           gAAATGTATGACAGATCACTATTTTTGAACCCTGTCACAGGACGTCATTATAGTGTGTGGCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCtt                                                                 <  2:379026/149‑1 (MQ=255)                                                                                  aTGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGAGCGCGTCCTTCATTTTg                                                          >  2:853111/1‑149 (MQ=255)                                                                                   tGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGt                                                         <  2:182112/149‑1 (MQ=255)                                                                                    gACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAATTATGGACCGCGTCCTTCAtt                                                             >  1:774026/1‑144 (MQ=255)                                                                                    gACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTa                                                        <  2:612839/149‑1 (MQ=255)                                                                                      ctgATCACTATTTTTGAATCCTGTCAGAGGACGTCATTATAGTGTGTGTCGGATCTTGTTTCGCTTCTGCATGTTACATATGATGGGCACGTAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACt                                                      <  2:303238/147‑1 (MQ=255)                                                                                      cAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCATCATAGTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACt                                                      >  2:706835/1‑149 (MQ=255)                                                                                      cAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAAGGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtttt                                                           <  1:678399/144‑1 (MQ=255)                                                                                           cACTATTTTTGAAGCCTGTCACATGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACt                                                      <  1:428000/144‑1 (MQ=255)                                                                                                             tCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTTATTTTGTACTGGCACTTGCCGTTGTTGc                                    <  1:66641/144‑1 (MQ=255)                                                                                                               acaGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGa                                  >  1:235549/1‑144 (MQ=255)                                                                                                                caGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTTCTGGCACTTGCCGTTGTTGCGATTCTCg                            <  2:626210/149‑1 (MQ=255)                                                                                                                  ggACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCa                          <  2:611521/149‑1 (MQ=255)                                                                                                                                 gtgtgtCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTaa                <  1:15967/144‑1 (MQ=255)                                                                                                                                 gtgtgtCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTaa                <  1:43820/144‑1 (MQ=255)                                                                                                                                   gtgtCAGATCTTGTTTTCCTTAACCATGCTACATAAAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAagc              >  1:925918/1‑144 (MQ=255)                                                                                                                                    tgtCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCActgcttaccagcagtgcg        <  2:397065/149‑14 (MQ=255)                                                                                                                                    tgtCAGATCTTGCTTTCCTTAAACATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAAGCAGCGAc        <  2:266182/149‑1 (MQ=255)                                                                                                                                     gtCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCActgcttaccagcagtgcga       <  2:727467/149‑15 (MQ=255)                                                                                                                                          aTCTTGTTTTCCTTAACCATGTTTCATAGAATGTGCAAGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGGTTCTCGCACTGCTGGTAAGCAGCGACCGCaaa  >  2:681436/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                 ATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAA  >  CP009273/2506313‑2506598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑