| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,707,318 | A→G | 12.0% | H355R (CAT→CGT) | srmB → | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,707,318 | 0 | A | G | 12.0% | 75.2 / 2.0 | 34 | H355R (CAT→CGT) | srmB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base A (10/19); new base G (2/2); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
TGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAA > CP009273/2707172‑2707457 | tGTTGCCGGGCGCGGCATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATg < 1:405355/149‑1 (MQ=255) tGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGAcc < 1:457304/149‑1 (MQ=255) gcgGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCGTGACCATctgctg > 1:448108/1‑149 (MQ=255) gcgGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgctg > 1:278757/1‑149 (MQ=255) gcgGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAAGTTCGCTGGTGGAAGCCCGTGACCACctgctg > 1:448112/1‑149 (MQ=255) tATCGACATTCCGGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTgg < 2:281676/149‑1 (MQ=255) ccGTCAGCCACGTCTTTAACTTCGATCTGCCGCGCAGTGGCGATACTTCTTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGTCCATCTGCTGCTGGGTAAAGTAGGCCGc < 1:257933/148‑1 (MQ=255) tCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCtat > 2:9843/1‑149 (MQ=255) acccACGTGTTGACCTTCGATATGCCGCGCAGTGGCGATCCTTATTCGCTCCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTatat < 2:281365/147‑1 (MQ=255) cGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTAAAGAACTATCCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAag > 1:322240/1‑149 (MQ=255) cactcggattggCCGCGCAGTGGCGATACGTATTTGCACCGTATCGGACGTACCACGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCCGGACCAGCTTCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAAt < 1:67677/138‑1 (MQ=255) tGCCGCGCCTTGGCGCTACTTCTTTGCACCGTTTCGGACGGCCCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTTCAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTcgc < 2:351368/149‑1 (MQ=255) ccGCGCAGTGGCGATACTTATTTGCCCCGTATCGGGCGAAACCCACGCGCCTGGCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGt > 1:297743/1‑149 (MQ=255) gcgcAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTa > 1:93370/1‑149 (MQ=255) ggCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgatga < 1:414682/149‑1 (MQ=255) gATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATGTTGCAGAGCCAATTAAAGCTCGCGTTATTGGTGAGtt > 1:10939/1‑149 (MQ=255) aTACTTATTTGCACCGTATCGGACGTGGCGCGCGGGCCGGTCGGAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTa < 2:294084/149‑1 (MQ=255) cTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGc < 1:3329/149‑1 (MQ=255) cTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATAGTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGc < 2:117337/149‑1 (MQ=255) cggactgcccGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCACGACCCTCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgcgc < 2:449405/141‑1 (MQ=255) cGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgcgc < 1:210086/149‑1 (MQ=255) gACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCa < 1:411993/149‑1 (MQ=255) ccGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGTGAAAGTAGGTCGCTATATTGATGTGTCAATTCTCGTTCGTGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGaa < 1:67463/149‑1 (MQ=255) gcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGc > 1:408352/1‑149 (MQ=255) cgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGcg > 1:193942/1‑148 (MQ=255) cGGGCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCg < 1:39205/149‑1 (MQ=255) tCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTACCGTAGGCCGCGATATTGGAGAGCCGATTAAAGCTCGCGTTATGGATGAGTAACGCCCGAAAACGCGGGCCCAAAGCGAAAAACAGACCGGCa > 2:94376/1‑149 (MQ=255) tCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACt < 2:108561/149‑1 (MQ=255) gcggtgagAAGCCCATGACCATCTGTTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTgg < 2:341254/142‑1 (MQ=255) cTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGACGCGAGTACTAGc > 1:359053/1‑149 (MQ=255) tggtggCAGCCCGTGACCATCGGCTGCTGGGTAAGGTAGGCCGCTATATTGAAGCGCCGATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCTAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCt < 2:448112/149‑1 (MQ=255) tggtggAAGCCCGTGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCt < 2:448108/149‑1 (MQ=255) tgggggAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCt < 2:278757/149‑1 (MQ=255) tggAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGCAGAGCCCATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTaaa < 1:295201/149‑1 (MQ=255) | TGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAA > CP009273/2707172‑2707457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |