| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,536,804 | T→C | 13.7% | F93F (TTT→TTC) | yhgA → | transposase_31 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,536,804 | 0 | T | C | 13.7% | 65.5 / 3.1 | 29 | F93F (TTT→TTC) | yhgA | transposase_31 family protein |
| Reads supporting (aligned to +/- strand): ref base T (16/9); new base C (2/2); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
TCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGTATTGAAAGCCCCTATCCCTATTCGCTGTGTTGGCTGGATTGTTTCG > CP009273/3536659‑3536946 | tCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTTCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAACGCACCCCAAACAAACTGATCGCATTTCGc > 1:45468/1‑149 (MQ=255) aGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCatg > 1:384870/1‑149 (MQ=255) tCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTCCGCa < 1:200915/139‑1 (MQ=255) tCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTCCGCa < 1:201208/139‑1 (MQ=255) tCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTCCGCa > 2:201208/1‑139 (MQ=255) tCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTCCGCa > 2:200915/1‑139 (MQ=255) cTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTg > 1:182377/1‑149 (MQ=255) gatgaCGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAAt < 2:282710/149‑1 (MQ=255) gAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGa < 1:53745/149‑1 (MQ=255) cTATTCCGAGGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTg < 2:292552/149‑1 (MQ=255) tgCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACg < 1:115421/149‑1 (MQ=255) ggTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGAtgg > 2:253276/1‑149 (MQ=255) agcAAGGCCCGGAATACGTCTAGTGTCTGTTTGAACATCAAAGCACTCCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGCGGCCGATGGTGGTGCCAtgg < 2:439987/147‑3 (MQ=255) ggACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAGTCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTgttgtt > 1:103209/1‑149 (MQ=255) aCCCGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGtttt < 1:263736/149‑1 (MQ=255) cAGGATACATCTATTGTCTGATTGAACACCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTAAGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTAc > 1:350170/1‑149 (MQ=255) aGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTGGGATGATGCGTTACGCTATTGCCGCAATGCAAAATCCCCGTGATGCAGGATACACACAGTTGCCGATGCTGGTGACATTGATGTTTTAcc > 2:342983/1‑149 (MQ=255) aCATCTATTGTCTGATTGAACATCATAGCTACTTAAGCGCGCGTATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGt < 1:437109/149‑1 (MQ=255) aCATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGt > 2:304169/1‑149 (MQ=255) tGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTACCGCAATGCAAAATCACCTTGATGCTGGATACAAAACATTGCTGATGCGGGTGCCATGGTTGTTTTACCACGGTATTGATAGcccc > 2:281204/1‑149 (MQ=255) gATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTGGCCGATGGTGGTGCCATTGTTGTTTTACCCCGGTATTGAAAGCccct > 1:90905/1‑149 (MQ=255) aaCATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACACAAAGTTGCCGATGGTGGTGCCATTGTTGTTTTTCCACGGTGTTGAAAGCccctatccc > 2:109329/1‑149 (MQ=255) aaCATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGTATTGAAAGCccctatccc > 1:390797/1‑149 (MQ=255) aaCATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGTATTGAAAGCccctatccc > 1:355845/1‑149 (MQ=255) aTCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGTATTGAAAGCccctatccctat > 1:105196/1‑149 (MQ=255) aTCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTCCCGCTATCCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGTTGCCATTGTTGTTTTACCACGGTATTGAAGACcccgttccctat > 2:194469/1‑149 (MQ=255) aaGCACCTCAAACAAACTGATCGCATATCGCATGATGCGATACGCTATTGCCGCAATGCACAATCACCTTGGTGCATGATACGAAACGTTGCCGAGGGTGGTGCGATGGATGTTTTACCACGGTATTGAAAGTCCCACTCCCTTTTCGc > 2:36290/1‑149 (MQ=255) aGCACCTCAAACAACCTGAACGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGTATTGAAAGCCCCTATCCCTATTCGCt < 2:292976/149‑1 (MQ=255) cGCAGTTCGCATGATGCGTTACGCTATTGCCGCTCAACAACAACACCGTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGTATTGAAAGCCCCTATTCCTATTCGCTGTGTTGGCTGGATTGTTTCg < 1:243703/149‑1 (MQ=255) | TCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATACATCTATTGTCTGATTGAACATCAAAGCACCTCAAACAAACTGATCGCATTTCGCATGATGCGTTACGCTATTGCCGCAATGCAAAATCACCTTGATGCTGGATACAAAACGTTGCCGATGGTGGTGCCATTGTTGTTTTACCACGGTATTGAAAGCCCCTATCCCTATTCGCTGTGTTGGCTGGATTGTTTCG > CP009273/3536659‑3536946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |